Shrikumar Vijaykumar Mahamuni, Nitin Sadashiv Shinde, Prakash Vitthal Wani, Ashok Sitaram Patil


Objective: The purpose of the present investigation was to assess antifungal characterization of Burkholderia gladioli strain VIMP03 (JQ867372), an
isolate from sugar beet rhizosphere.
Methods: Antifungal characterization was carried out by biochemical, dual culture, and agar well diffusion methods against Ceratocystis paradoxa, a
soilborne pathogen of sugarcane and other crops. Culture filtrate and ethyl acetate extract obtained from culture supernatant were analyzed by highperformance
liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) analyses, respectively.
Results: The culture filtrate and ethyl acetate extract exhibited effective antifungal activity. Organic acid profile of the culture filtrate was determined.
Acetic acid was mainly produced by the culture under study. The GC-MS analysis of ethyl acetate extract documented the presence of compounds
including tetratetracontane, 9-nonadecene, erucic acid, and other hydrocarbon derivatives.
Conclusion: The GC-MS, HPLC, and biochemical profiles of B. gladioli strain VIMP03 (JQ867372) revealed its agro clinical-antifungal potential.
Keywords: Antifungal, Burkholderia gladioli, High-performance liquid chromatography, Gas chromatography-mass spectrometry.

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Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 3 March 2017 Page: 221-226

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Authors & Affiliations

Shrikumar Vijaykumar Mahamuni
Shardabai Pawar Mahila College (Affiliated to Savitribai Phule Pune University)

Nitin Sadashiv Shinde
Shardabai Pawar Mahila College (Affiliated to Savitribai Phule Pune University)

Prakash Vitthal Wani
Mahatma Phule Krishi Vidyapeeth, Rahuri, 413722, Maharashtra, India.

Ashok Sitaram Patil
Vasantdada Sugar Institute, Manjari Bk., Pune, 412307, Maharashtra, India.

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