Vasundra Devi Devaraj, Suja Samiappan


Objective: To evaluate the antioxidant and chemotherapeutic potential of Curcuma amada Rhizome extract on benzo(a)pyrene (BaP) induced cervical
carcinoma in Sprague Dawley rats.
Methods: A total of 30 female Sprague Dawley rats were selected to establish cervical cancer model and then divided into 5 groups at random with
six mice in each group. Group 1 control, Group 2 BaP (oral), Group 3 BaP for 8 weeks and post-treated with cisplatin (intravenous administration),
Group 4 BaP for 8 weeks and post-treated with 250 mg of ethanol extract of C. amada (oral), Group 5 BaP for 8 weeks and post-treated with 500 mg
of ethanol extract of C. amada (oral). 4 weeks after the treatment, the animals were sacrificed, serum separated, and cervical tissues were dissected.
Antioxidants and the markers carcinoembryonic antigen (CEA), cancer antigens (CAs) 125, gamma glutamyltransferase (GTT) were assayed in serum
and the tissue was used for analyzing tumor burden and sectioned for histopathological assays.10% tissue homogenate was estimated for antioxidants
and membrane-bound enzymes.
Results: BaP treated group showed significant (p<0.001) incidence of tumor burden, decreased activities of antioxidants, elevated lipid peroxidation,
Na+/K+ adenosine triphosphatase (Na+K+ATPase), Calcium adenosine triphosphatase (Ca2+ATPase), Magnesium adenosine triphosphatase (Mg2+ ATPase),
CEA, CA 125, GTT. Treatment with C. amada rhizome extract and standard drug cisplatin reverted the antioxidants, serum markers and tissue enzymes.
Conclusion: From the results, it can be concluded that C. amada Rhizome extract ameliorated BaP induced oxidative stress in the cervical
carcinogenicity of rats.
Keywords: Curcuma amada, In vivo antioxidant, Chemotherapy, Benzo(a)pyrene, Cervical carcinoma, Tumor markers.

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Brys M, Semczuk A, Wójcik M, Krajewska WM, Jakowicki JA. p53 protein detection by the western blotting technique in normal and neoplastic specimens of human endometrium. Cancer Lett 2000;148(2):197-205.

Thielen A, Klus H, Müller L. Tobacco smoke: Unraveling a controversial subject. Exp Toxicol Pathol 2008;60(2-3):141-56.

Park JH, Mangal D, Frey AJ, Harvey RG, Blair IA, Penning TM. Arylhydrocarbon receptor facilitates DNA strand breaks and 8-oxo-2-deoxyguanosine formation by the aldo-ketoreductase product benzo[a]pyrene-7,8-dione. J BiolChem 2009;284:29725-34.

Wang C, Zhao Y, Zheng R, Ding X, Wei W, Zuo Z, et al. Effects of tributyltin, benzo[a]pyrene, and their mixture on antioxidant defense systems in Sebastiscus marmoratus. Ecotoxicol Environ Saf 2006;65(3):381-7.

Alam S, Conway MJ, Chen HS, Meyers C. The cigarette smoke carcinogen benzo[a]pyrene enhances human papillomavirus synthesis. J Virol 2008;82(2):1053-8.

Wojcik M, Burzynska-Pedziwiatr I, Wozniak LA. A review of natural and synthetic antioxidants important for health and longevity. Curr Med Chem 2010;17(28):3262-88.

Kunnumakkara AB, Anand P, Aggarwal BB. Curcumin inhibits proliferation, invasion, angiogenesis and metastasis of different cancers through interaction with multiple cell signaling proteins. Cancer Lett 2008;269(2):199-225.

Aggarwal BB. The past, present and future of multi-targeted cancer treatment “naturally”: Food for thought. Cancer Lett 2008;269(2):187-8.

Garry S, Nesslany F, Aliouat E, Haguenoer JM, Marzin D. Hematite (Fe(2)O(3)) enhances benzo[a]pyrene genotoxicity in endotracheally treated rat, as determined by Comet Assay. Mutat Res 2003;538(1-2):19-29.

Das K, Samanta L, Chainy GB. A modified spectrophotometric assay of superoxide dismutase using nitrite formation by superoxide radicals. Indian J Biochem Biophys 2000;37:201-4.

Sinha AK. Colorimetric assay of catalase. Anal Biochem 1972;47(2):389-94.

Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959;82(1):70-7.

Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochim Biophys Acta 1979;582(1):67-78.

Omaye ST, Turnbull JD, Sauberlich HE. Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods Enzymol 1979;62:3-11.

Bishayee S, Balasubramanian AS. Lipid peroxide formation in rat brain. J Neurochem 1971;18(6):909-20.

Bonting SL. Sodium-potassium activated adenosine triphosphatase and cation transport. In: Bittar EE, editor. Membranes and Ion Transport. Vol. 1. London: Wiley-Interscience; 1970. p. 257-363.

Hjertén S, Pan H. Purification and characterization of two forms of a low-affinity Ca2 -ATPase from erythrocyte membranes. Biochim Biophys Acta 1983;728(2):281-8.

Ohnishi T, Suzuki T, Suzuki Y, Ozawa K. A comparative study of plasma membrane Mg2 -ATPase activities in normal, regenerating and malignant cells. Biochim Biophys Acta 1982;684(1):67-74.

Basu A, Imrhan V. Tomatoes versus lycopene in oxidative stress and carcinogenesis: Conclusions from clinical trials. Eur J Clin Nutr 2007;61(3):295-303.

Franck G, Wdzieczak-Bakala J, Henrotte JG. Modulating role of glucose on magnesium transport in rat erythrocytes. J Nutr Biochem 1999;10(7):433-7.

Srikanth S. Spectrum of cervical lesions observed in 500 cases: Carcinoma cervix the leading cause of death in females. Indian J Cancer 2016;53(1):61-2.

Holschneider CH, Baldwin RL, Tumber K, Aoyama C, Karlan BY. The fragile histidine triad gene: A molecular link between cigarette smoking and cervical cancer. Clin Cancer Res 2005;11(16):5756-63.

Kryston TB, Georgiev AB, Pissis P, Georgakilas AG. Role of oxidative stress and DNA damage in human carcinogenesis. Mutat Res 2011;711(1-2):193-201.

Liou GY, Storz P. Reactive oxygen species in cancer. Free Radic Res 2010;44(5):479-96.

Diwanay S, Chitre D, Patwardhan B. Immunoprotection by botanical drugs in cancer chemotherapy. J Ethnopharmacol 2004;90(1):49-55.

Huang WY, Cai YZ, Zhang Y. Natural phenolic compounds from medicinal herbs and dietary plants: Potential use for cancer prevention. Nutr Cancer 2010;62(1):1-20.

Pednekar PP, Vakil BV, Sane RT, Datar AG. Phytochemical profile and antioxidant activity of the essential oil from Blumea eriantha Dc. Int J Pharm Pharm Sci 2013;5(1):404-13.

Soobrattee MA, Bahorun T, Aruoma OI. Chemopreventive actions of polyphenolic compounds in cancer. Biofactors 2006;27(1-4):19-35.

Srivastava AK, Srivastava SK, Shah NC. Constituents of the rhizome essential oil of Curcuma amada Roxb. from India. J Essent Oil Res 2009;13:63-4.

Faiz Hossain C, Al-Amin M, Rahman KM, Sarker A, Alam MM, Chowdhury MH, et al. Analgesic principle from Curcuma amada. J Ethnopharmacol 2015;163:273-7.

Bai KC, Shannukanada P. Analgesic activity of aqueous extract of Curcuma amada (mango-ginger) in male albino Wister rats. J Evid Based Med Healthc 2015;2:6662.

Devi PH, Mazunder PB, Devi PL. Antioxidant and antimutagenic activity of Curcuma caesia Roxb, rhizome extracts. Toxicol Rep 2015;2:423-8.

Upadhyay A, Sharma RK, Singh G, Jain AK. Evaluation of anti-tumor activity of Curcuma amada Roxb. Rhizome. Int J Sci Eng Res 2013;4(4):238-42.

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Palanisamy and Samiappan

Wu YQ, Wang CG, Wang Y, Zhao Y, Chen YX, Zuo ZH. Antioxidant responses to benzo[a]pyrene, tributyltin and their mixture in the spleen of Sebasticus marmoratus. J Environ Sci (China) 2007;19(9):1129-35.

Chena J, Songa M, Lia Y, Zhanga Y, Tayab K, Lia CM. The effect of phytosterol protects rats against 4-nitrophenol-induced liver damage. Environ Toxicol Pharmacol 2016;41:266-71.

Vieira LR, Sousa A, Frasco MF, Lima I, Morgado F, Guilhermino L. Acute effects of Benzo[a]pyrene, anthracene and a fuel oil on biomarkers of the common goby Pomatoschistus microps (Teleostei, Gobiidae). Sci Total Environ 2008;395(2-3):87-100.

Besaratinia A, Kim SI, Bates SE, Pfeifer GP. Riboflavin activated by ultraviolet A1 irradiation induces oxidative DNA damage-mediated mutations inhibited by vitamin C. Proc Natl Acad Sci U S A 2007;104(14):5953-8.

Maynard S, Schurman SH, Harboe C, de Souza-Pinto NC, Bohr VA. Base excision repair of oxidative DNA damage and association with cancer and aging. Carcinogenesis 2009;30(1):2-10.

Nijveldt RJ, van Nood E, van Hoorn DE, Boelens PG, van Norren K, van Leeuwen PA. Flavonoids: A review of probable mechanisms of action and potential applications. Am J Clin Nutr 2001;74(4):418-25.

Ramakrishnan G, Augustine TA, Jagan S, Vinodhkumar R, Devaki T. Effect of silymarin on N-nitrosodiethylamine induced hepatocarcinogenesis in rats. Exp Oncol 2007;29(1):39-44.

Vinodhkumar R, Ravikumar V, Shivashangari KS, Kamaraj S, Devaki T. Chemopreventive role of Lycopene and D-arginine in benzo(A)pyrene induced lung cancer with reference to lipid peroxidation, antioxidant system and tumour marker enzymes. Int J Cancer Res 2006;2(3):224-33.

Selvendiran K, Singh JP, Krishnan KB, Sakthisekaran D. Cytoprotective effect of piperine against benzo[a]pyrene induced lung cancer with reference to lipid peroxidation and antioxidant system in Swiss albino mice. Fitoterapia 2003;74(1-2):109-15.

Rozanida AR, NurulIzza N, Mohd Helme NH, Zanariah HA. Cosmeceutical product from species in the family Zingiberaceae. In: Mazura MP, editor. Harnessing Cures from Nature: Trends and Prospects. Kepong, Selangor, Malaysia: Forest Research Institute; 2006. p. 31-6.

Yingming P, Ping L, Hengshan W, Min L. Antioxidant activities of several Chinese medicinal herbs. Food Chem 2004;88:347-50.

Sahu R, Saxena J. Phytochemical analysis of Curcuma amada by FTIR and UV-VIS spectroscopic analysis. Int J Pharm Res Sch 2015;4(1-2):458-61.

Singh S, Gupta AK. Evaluation of phenolics content, flavonoids and antioxidant activity of Curcuma amada (Mango Ginger) and Zingiber officinale (Ginger). Research and Reviews. J Chem 2013;2(1):32-5.

Yu Z, Wang R, Xu L, Xie S, Dong J, Jing Y. ß-Elemene piperazine derivatives induce apoptosis in human leukemia cells through down regulation of c-FLIP and generation of ROS. PLoS One 2011;6(1):e15843.

Policegoudra RS, Aradhya SM, Singh L. Mango ginger (Curcuma amada Roxb.) – a promising spice for phytochemicals and biological activities. J Biosci 2011;36(4):739-48.

Phongpradist R, Chaiyana W, Anuchapreeda A. Curcumin-loaded multi-valent ligands conjugated nanoparticles for anti-inflammatory activity. Int J Pharm Pharm Sci 2015;7(4):203-8.

Liu RH. Potential synergy of phytochemicals in cancer prevention: Mechanism of action. J Nutr 2004;134(12 Suppl):3479S-85S.

Vishnupriya M, Nishaa S, Sasikumar JM, Gopalakrishnan VK. Antioxidant activity and hydroxyl radical induced DNA damage protection effect of aqueous extract of Curcuma amada Roxb. Res J Pharm Biol Chem Sci 2012;3(4):89-96.

Sivaprabha J. Radical scavenging activity of leaves and rhizomes of Curcuma amada. Int J Pharm Res Dev 2011;3:167-74.

Policegoudra RS, Abiraj K, Channe Gowda D, Aradhya SM. Isolation and characterization of antioxidant and antibacterial compound from mango ginger (Curcuma amada Roxb.) rhizome. J Chromatogr B Analyt Technol Biomed Life Sci 2007;852(1-2):40-8.

Sahu RK, Nahak G. Evaluation of antioxidant activity in ethanolic extracts of five Curcuma species. Int Res J Pharm 2011;2(12):243-8.

Siegel EM, Salemi JL, Villa LL, Ferenczy A, Franco EL, Giuliano AR. Dietary consumption of antioxidant nutrients and risk of incident cervical intraepithelial neoplasia. Gynecol Oncol 2010;118:289-94.

Nair J, Srivatanakul P, Haas C, Jedpiyawongse A, Khuhaprema T, Seitz HK, et al. High urinary excretion of lipid peroxidation-derived DNA damage in patients with cancer-prone liver diseases. Mutat Res 2010;683(1-2):23-8.

Kim HS, Kwack SJ, Lee BM. Lipid peroxidation, antioxidant enzymes, and benzo[a]pyrene-quinones in the blood of rats treated with benzo[a]pyrene. Chem Biol Interact 2000;127(2):139-50.

Pan LQ, Ren J, Liu J. Responses of antioxidant systems and LPO level to benzo(a)pyrene and benzo(k)fluoranthene in the haemolymph of the scallop Chlamys ferrari. Environ Pollut 2006;141(3):443-51.

Amresh G, Rao CV, Singh PN. Antioxidant activity of Cissampelos pareira on benzo(a)pyrene-induced mucosal injury in mice. Nutr Res 2007;27:625-32.

Policegoudra RS, Rehna K, Rao LJ, Aradhya SM. Antimicrobial, antioxidant, cytotoxicity and platelet aggregation inhibitory activity of a novel molecule isolated and characterized from mango ginger (Curcuma amada Roxb.) rhizome. J Biosci 2010;35:231-40.

Tarwadi K, Agte V. Antioxidant and micronutrient quality of fruit and root vegetables from the Indian subcontinent and their comparative performance with green leafy vegetables and fruits. J Sci Food Agric 2005;85:1469-76.

Vishnukumar S, Rajadurai M, Stephan R, Chandra S. Effect of morin on glycoproteins and membrane bound enzymes in streptozotocin-induced diabetic rats. J Acad Ind Res 2012;1:5-10.

Yatime L, Buch-Pedersen MJ, Musgaard M, Morth JP, Winther AM, Pedersen BP. P-type ATPases as drug targets: Tools for medicine and science. Biochem Biophys Acta 2009;1787:207-20.

McNulty HP, Byun J, Lockwood SF, Jacob RF, Mason RP. Differential effects of carotenoids on lipid peroxidation due to membrane interactions: X-ray diffraction analysis. Biochim Biophys Acta 2007;1768(1):167-74.

Park JS, Chyun JH, Kim YK, Line LL, Chew BP. Astaxanthin decreased oxidative stress and inflammation and enhanced immune response in humans. Nutr Metab 2010;7:18.

Kolanjiappan K, Manoharan S, Kayalvizhi M. Measurement of erythrocyte lipids, lipid peroxidation, antioxidants and osmotic fragility in cervical cancer patients. Clin Chim Acta 2002;326(1-2):143-9.

Bhagavathy S, Sumathi P. Stabilization of membrane bound ATPases and lipid peroxidation by carotenoids from Chlorococcum humicola in Benzo(a)pyrene induced toxicity. Asian Pac J Trop Biomed 2012;2:380-4.

Ithayarasi AP, Devi CS. Effect of alpha-tocopherol on lipid peroxidation in isoproterenol induced myocardial infarction in rats. Indian J Physiol Pharmacol 1997;41(4):369-76.

Denissenko MF, Pao A, Tang M, Pfeifer GP. Preferential formation of benzo[a]pyrene adducts at lung cancer mutational hotspots in P53. Science 1996;274(5286):430-2.

Sajid KM, Chaouachi K, Mahmood R. Hookah smoking and cancer: Carcinoembryonic antigen (CEA) levels in exclusive/ever hookah smokers. Harm Reduct J 2008;5:19.

Ueda Y, Enomoto T, Kimura T, Miyatake T, Yoshino K, Fujita M, et al. Serum biomarkers for early detection of gynecologic cancers. Cancers (Basel) 2010;2(2):1312-27.

Huang MT, Ma W, Lu YP, Chang RL, Fisher C, Manchand PS, et al. Effects of curcumin, demethoxycurcumin, bisdemethoxycurcumin and tetrahydrocurcumin on 12-O-tetradecanoylphorbol-13-acetate-induced tumor promotion. Carcinogenesis 1995;16(10):2493-7.

Gadducci A, Cosio S, Carpi A, Nicolini A, Genazzani AR. Serum tumor markers in the management of ovarian, endometrial and cervical cancer. Biomed Pharmacother 2004;58(1):24-38.

Patlolla JM, Rao CV. Triterpenoids for cancer prevention and treatment: Current status and future prospects. Curr Pharm Biotechnol 2012;13:147-55.

Grimm C, Hofstetter G, Aust S, Mutz-Dehbalaie I, Bruch M, Heinze G, et al. Association of gamma-glutamyltransferase with severity of disease at diagnosis and prognosis of ovarian cancer. Br J Cancer 2013;109:610-4.

Fentiman IS. Gamma-glutamyl transferase: Risk and prognosis of cancer. Br J Cancer 2012;106:1467-8.

Kim KI, Kim JW, Hong BS, Shin DH, Cho HY, Kim HK, et al. Antitumor, genotoxicity and anticlastogenic activities of polysaccharide from Curcuma zedoaria. Mol Cells 2000;10:392-8.

Yee H, Yie TA, Goldberg J, Wong KM, Rom WN. Immunohistochemical study of fibrosis and adenocarcinoma in dominant-negative p53 transgenic mice exposed to chrysotile asbestos and benzo(a)pyrene. J Environ Pathol Toxicol Oncol 2008;27:267-76.

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Asian Journal of Pharmaceutical and Clinical Research
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Vasundra Devi Devaraj
Bharathiar University Coimbatore Tamilnadu

Suja Samiappan

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