ISOLATION AND CHARACTERIZATION OF ENDOPHYTIC FUNGI FROM CALOTROPIS PROCERA FOR THEIR ANTIOXIDANT ACTIVITY


Vipin Nagda, Archana Gajbhiye, Dinesh Kumar Chhatwani

Abstract


ABSTRACT
Objective: To investigate the antioxidant activity of methanolic extract of endophytic fungi isolated from Calotropis procera plant.
Methods: The endophytic fungi were isolated from explants of leaf, stem, and flower of C. procera on potato dextrose broth (PDB) medium. The fungal
isolates were mass cultured in PDB. The methanolic extracts of these fungi were prepared and evaluated for the antioxidant activity by 1,1-diphenyl-
2-picryl-hydrazyl (DPPH) free radical scavenging assay and reducing power assay. The extracts were characterized for the presence of phenolics and
flavonoids.
Results: A total of 8 endophytic fungi were isolated from C. procera explants. Out of these 8 fungal isolates, the crude extracts of two fungi, i.e. CPIMR-2
and CPIL-1 showed positive antioxidant activity. From the morphological characteristics, the isolates CPIMR-2 and CPIL-1 were identified as Penicillium
spp. and Aspergillus spp., respectively. Both the isolates were found to possess antioxidant potential with % inhibition value of 81.16% and 80.97%
in the DPPH radical scavenging assay. The phytochemical screening of the methanolic extracts showed the presence of phenolics and flavonoids. The
total flavonoids content in Penicillium species and Aspergillus species were found to be 130.50 μg/mg and 94.91 μg/mg of extracts, respectively. The
total phenolic content was found to be 9.16 μg/mg and 12.13 μg/mg of extracts in Penicillium species and Aspergillus species, respectively.
Conclusions: Endophtyic fungi were found to be present in medicinal value plant C. procera. The probable bioactive component for antioxidant
activity possessed by the fungi would be the flavonoids and phenolics. These metabolite produced by endophytic fungi from C. procera need to be
explored further as potential source of novel natural antioxidant compound.
Keywords: Endophytes, Calotropis procera, Antioxidant, Radical scavenging, Reducing power, Flavonoids.


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About this article

Title

ISOLATION AND CHARACTERIZATION OF ENDOPHYTIC FUNGI FROM CALOTROPIS PROCERA FOR THEIR ANTIOXIDANT ACTIVITY

DOI

10.22159/ajpcr.2017.v10i3.16125

Date

01-03-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 3 March 2017 Page: 254-258

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

335 Views | 89 Downloads

Authors & Affiliations

Vipin Nagda
Sir Padampat Singhania University
India

Archana Gajbhiye
Sir PadampatSinghania University
India

Dinesh Kumar Chhatwani
India


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