SILVER NANOPARTICLES FROM TRIANTHEMA PORTULACASTRUM: GREEN SYNTHESIS, CHARACTERIZATION, ANTIBACTERIAL AND ANTICANCER PROPERTIES
Objective: In this study, silver nanoparticles (SNPs) were synthesized using an aqueous extract of Trainthema portulacastrum and silver ions (Ag+)
which have been proven against certain pathogenic bacterial strains and hepatocellular carcinoma (HepG2) cell line.
Methods: The bio fabricated nanoparticles were confirmed by surface plasmon resonance which were characterized by biophysical measures
utilizing the ultraviolet-visible spectroscopy, scanning electron microscopy (SEM), energy dispersive X-ray, and transmission electron microscope
(TEM), Fourier transform infrared spectroscopy, particle size analyzer, and X-ray diffraction. Antibacterial efficacy against Enterobacter aerogens,
Proteus mirabilis, Escherichia coli, Staphylococcus epidermis, and Bacillus subtilis. The effect of SNPs tested against HepG2 and NIH/3T3 cell line
exhibits a dose-dependent toxicity.
Results and Conclusion: The SEM and TEM images confirmed the presence of spherical and hexagonal shape (0.3-4 Î¼m) of nanocrystalline particles
with the size range of 11.5-29.2 nm. The average particles size of SNPs is 190.3Â±17.0 nm. Antibacterial activity was carried out by agar well diffusion
method against different pathogenic bacteria of which B. subtilis showed a significant zone of inhibition 8.66 mm and 12.0 mm for aqueous plant
extract and synthesized SNPs. The effect of SNPs tested against HepG2 and NIH/3T3 cell line exhibits a dose-dependent toxicity. In case of HepG2, the
cell viability was decreased to 50% (IC50) at the concentration of 173.8Â±0.84 Î¼g/mL. From the results, it can be concluded that the SNPs fabricated
using green synthesis method will be a promising candidate in the biomedical field, due to its high bioactive properties.
Keywords: Silver nanoparticles, Trainthema portulacastrum, Antibacterial activity, Cytotoxic activity.
1. Gardea-Torresdey JL, Parson JG, Gomez E, Peralta-Videa J, Troiani HE, Santiago P, et al. Formation and growth of Au nanoparticles in live side live Alfalfa plants. Nano Lett 2002;2:397-401.
2. Claus P, Hofmeister H. Electron microscopy and catalytic study of silver catalysts: Structure sensitivity of the hydrogenation of crotonaldehyde. J Phys Chem B 1999;103(14):2766-75.
3. CorrÃªa JM, Mori M, Sanches HL, da Cruz AD, Poiate E Jr, Poiate IA. Silver nanoparticles in dental biomaterials. Int J Biomater 2015;2015:485275.
4. Lakshmi PT, Priyanka D, Annamalai A. Reduction of silver ions by cell free extracts of Westiellopsis sp. Int J Biomater 2015;2015:539494.
5. Martinez-Gutierrez F, Olive PL, Banuelos A, Orrantia E, Nino N, Sanchez EM, et al. Synthesis, characterization, and evaluation of antimicrobial and cytotoxic effect of silver and titanium nanoparticles. Nanomedicine 2010;6(5):681-88.
6. Sharma VK, Yngard RA, Lin Y. Silver nanoparticles: Green synthesis and their antimicrobial activities. Adv Colloid Interface Sci 2009;145(1-2):83-96.
7. Kumar DA, Palanichamy V, Roopan SM. Green synthesis of silver nanoparticles using Alternanthera dentata leaf extract at room temperature and their antimicrobial activity. Spectrochim Acta A Mol Biomol Spectrosc 2014;127:168-71.
8. Baharara J, Namvar F, Ramezani T, Hosseini N, Mohamad R. Green synthesis of silver nanoparticles using Achillea biebersteinii flower extract and its anti-angiogenic properties in the rat aortic ring model. Molecules 2014;19(4):4624-34.
9. Arunachalam KD, Annamalai SK. Chrysopogon zizanioides aqueous extract mediated synthesis, characterization of crystalline silver and gold nanoparticles for biomedical applications. Int J Nanomedicine 2013;8:2375-84.
10. Sahana R, Daniel SC, Sankar SG, Archunan G, Vennison SJ, Sivakumar M. Formulation of bactericidal cold cream against clinical pathogens using Cassia auriculata flower extract-synthesized Ag nanoparticles. Green Chem Lett Rev 2014;7(1):64-72.
11. Krithiga N, Rajalakshmi A, Jayachitra A. Green synthesis of silver nanoparticles using leaf extracts of Clitoria ternatea and Solanum nigrum and study of its antibacterial effect against common nosocomial pathogens. J Nanosci 2015;2015:1-8.
12. Yallappa S, Manjanna J, Peethambar SK, Rajeshwara AN, Satyanarayan ND. Green synthesis of silver nanoparticles using Acacia farnesiana (sweet acacia) seed extract under microwave irradiation and their biological assessment. J Cluster Sci 2013;24(4):1081-92.
13. Deepshikha H, Alakesh P, Eramoni S, Bolin C. Phytochemical screening and synthesis of silver nanoparticles using leaf extract of Rhynchotechum ellipticum. Int J Pharm Pharm Sci 2014;6(1):672-74.
14. Ranjitham AM, Suja R, Caroling G, Tiwari S. In vitro evaluation of antioxidant, antimicrobial, anticancer activities and characterisation of Brassica oleracea. Var. Bortrytis. l synthesized silver nanoparticles. Int J Pharm Pharm Sci 2013;5(4):239-54.
15. Raj RS, Divya S, Sindhu S, Kasinathan K, Arumugam P. Studies on synthesis, characterization and application of silver nanoparticles using Mimosa pudica leaves. Int J Pharm Pharm Sci 2014;6(2):453-55.
16. Ashokkumar S, Ravi S, Velmurugan S. Green synthesis of silver nanoparticles from Gloriosa superba L. Leaf extract and their catalytic activity. Spectrochim Acta A Mol Biomol Spectrosc 2013;115:388-92.
17. Merin DD, Prakash S, Bhimba BV. Antibacterial screening of silver nanoparticles synthesized by marine micro algae. Asian Pac J Trop Med 2010;3(10):797-99.
18. Mariselvam R, Ranjitsingh AJ, Usha Raja Nanthini A, Kalirajan K, Padmalatha C, Mosae Selvakumar P. Green synthesis of silver nanoparticles from the extract of the inflorescence of Cocos nucifera (Family: Arecaceae) for enhanced antibacterial activity. Spectrochim Acta A Mol Biomol Spectrosc 2014;129:537-41.
19. Ghozali SZ, Vuanghao L, Ahmad NH. Biosynthesis and characterization of silver nanoparticles using Catharanthus roseus leaf extract and its proliferative effects on cancer cell lines. J Nanomed Nanotechnol 2015;6:305.
20. Gupta S, Jangir OP, Sharma M. The green synthesis, characterization and evaluation of antioxidant and antimicrobial efficacy of silver and gold nanospheres synthesized using wheat bran. Asian J Pharm Clin Res 2016;9(6):103-5.
21. Chandra VS. Phytochemical studies on leaves of Trianthema portulacastrum L. BIOS 2013;1:67-73.
22. Singh V, Tyagi A, Chauhan PK, Kumari P, Kaushal S. Identification and prevention of bacterial contamination on explant used in plant tissue culture labs. Int J Pharm Pharm Sci 2011;3(4):160-63.
23. Aziz N, Fatma T, Varma A, Prasad R. Biogenic synthesis of silver nanoparticles using Scenedesmus abundans and evaluation of their antibacterial activity. J Nanopart 2014;2014:6.
24. Shankar KG, Fleming TA, Vidhya R, Pradhan N. Synergistic efficacy of three plant extracts, Bergenia ciliata, Acorus calamus and Dioscorea bulbifera for antimicrobial activity. Int J Pharm Bio Sci 2016;7(4):(B)619-28.
25. Arokiyaraj S, Arasu MV, Vincent S, Prakash NU, Choi SH, Oh YK, et al. Rapid green synthesis of silver nanoparticles from Chrysanthemum indicum L and its antibacterial and cytotoxic effects: An in vitro study. Int J Nanomedicine 2014;9:379-88.
26. Lee YH, Cheng FY, Chiu HW, Tsai JC, Fang CY, Chen CW, et al. Cytotoxicity, oxidative stress, apoptosis and the autophagic effects of silver nanoparticles in mouse embryonic fibroblasts. Biomaterials 2014;35(16):4706-15.
27. Gurunathan S, Raman J, Abd Malek SN, John PA, Vikineswary S. Green synthesis of silver nanoparticles using Ganoderma neojaponicum Imazeki: A potential cytotoxic agent against breast cancer cells. Int J Nanomedicine 2013;8:4399-413.
28. Kawata K, Osawa M, Okabe S. In vitro toxicity of silver nanoparticles at noncytotoxic doses to HepG2 human hepatoma cells. Environ Sci Technol 2009;43(15):6046-51.
29. Syed A, Saraswati S, Kundu GC, Ahmad A. Biological synthesis of silver nanoparticles using the fungus Humicola sp. and evaluation of their cytoxicity using normal and cancer cell lines. Spectrochim Acta A Mol Biomol Spectrosc 2013;114:144-7.
30. Veerasamy R, Xin TZ, Gunasagaran S, Xiang TF, Yang EF, Jeyakumar N, et al. Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities. J Saudi Chem Soc 2011;15(2):113-20.
31. Filippo E, Serra A, Buccolieri A, Manno D. Green synthesis of silver nanoparticles with sucrose and maltose: Morphological and structural characterization. J Non Cryst Solids 2010;356(6-8):344-50.
32. Khalil MM, Ismail EH, El-Baghdady KZ, Mohamed D. Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity. Arabian J Chem 2014;7(6):1131-9.
33. Maciollek A, Ritter H. One pot synthesis of silver nanoparticles using a cyclodextrin containing polymer as reductant and stabilizer. Beilstein J Nanotechnol 2014;5(1):380-5.
34. Xu H, Kall M. Morphology effects on the optical properties of silver nanoparticles. J Nanosci Nanotechnol 2002;4(3):254-9.
35. Chandran SP, Chaudhary M, Pasricha R, Ahmad A, Sastry M. Synthesis of gold nanotriangles and silver nanoparticles using Aloe vera plant extract. Biotechnol Prog 2006;22(2):577-83.
36. Banu AN, Balasubramanian C, Moorthi PV. Biosynthesis of silver nanoparticles using Bacillus thuringiensis against dengue vector, Aedes aegypti (Diptera: Culicidae). Parasitol Res 2014;113(1):311-6.
37. Mollick MM, Rana D, Dash SK, Chattopadhyay S, Bhowmick B, Maity D, et al. Studies on green synthesized silver nanoparticles using Abelmoschus esculentus (L.) Pulp extract having anticancer (in vitro) and antimicrobial applications. Arabian J Chem 2015. DOI: 10.1016/j.arabjc.2015.04.033.
38. Gao FP, Zhang HZ, Liu LR, Wang YS, Jiang Q, Yang XD, et al. Preparation and physicochemical characteristics of self-assembled nanoparticles of deoxycholic acid modified carboxymethyl curdlan conjugates. Carbohydr Polym 2008;71:606-13.
39. Rosarin FS, Arulmozhi V, Nagarajan S, Mirunalini S. Antiproliferative effect of silver nanoparticles synthesized using amla on Hep2 cell line. Asian Pac J Trop Med 2013;6(1):1-10.
40. Stoikov II, Yushkova EA, Antipin IS, Konovalov AI. Synthesis of silver and lithium sub-micro-and nanoparticles coated with derivatives of p-tert-butyl thiacalixarenes. J Nanopart Res 2011;13(12):6603-11.
41. Satyavani K, Gurudeeban S, Ramanathan T, Balasubramanian T. Biomedical potential of silver nanoparticles synthesized from calli cells of Citrullus colocynthis (L.) Schrad. J Nanobiotechnology 2011;9:43.
42. Banerjee P, Satapathy M, Mukhopahayay A, Das P. Leaf extract mediated green synthesis of silver nanoparticles from widely available Indian plants: Synthesis, characterization, antimicrobial property and toxicity analysis. Bioresour Bioprocess 2014;1(3):1-10.
43. Prathna TC, Chandrasekaran N, Raichur AM, Mukherjee A. Biomimetic synthesis of silver nanoparticles by Citrus limon (lemon) aqueous extract and theoretical prediction of particle size. Colloids Surf B Biointerfaces 2011;82(1):152-9.
44. Pourmortazavi SM, Taghdiri M, Makari V, Rahimi-Nasrabadi M. Procedure optimization for green synthesis of silver nanoparticles by aqueous extract of Eucalyptus oleosa. Spectrochim Acta A Mol Biomol Spectrosc 2015;136:1249-54.
45. Sulaiman GM, Mohammed WH, Marzoog TR, Al-Amiery AA, Kadhum AA, Mohamad AB. Green synthesis, antimicrobial and cytotoxic effects of silver nanoparticles using Eucalyptus chapmaniana leaves extract. Asian Pac J Trop Biomed 2013;3(1):58-63.
46. Kaviya S, Santhanalakshmi J, Viswanathan B. Green synthesis of silver nanoparticles using Polyalthia longifolia leaf extract along with D-sorbitol: Study of antibacterial activity. J Nanotechnol 2011;2011:1-5.
47. Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, RamÃrez JT, et al. The bactericidal effect of silver nanoparticles. Nanotechnology 2005;16(10):2346-53.
48. Ghosh P, Han G, De M, Kim CK, Rotello VM. Gold nanoparticles in delivery applications. Adv Drug Deliv Rev 2008;60(11):1307-15.
49. Prashanth S, Menaka I, Muthezhilan R, Kumar SN. Synthesis of plant-mediated silver nano particles using medicinal plant extract and evaluation of its anti microbial activities. Int J Eng Sci Technol 2011;3:6235-50.
50. Awwad AM, Salem NM, Abdeen AO. Green synthesis of silver nanoparticles using carob leaf extract and its antibacterial activity. Int J Ind Chem 2013;4(29):2-7.
51. Vanaja M, Paulkumar K, Gnanajobitha G, Rajeshkumar S, Malarkodi C, Annadurai G. Herbal plant synthesis of antibacterial silver nanoparticles by Solanum trilobatum and its characterization. Int J Met 2014;2014:8.
52. Geethalakshmi R, Sarada DV. Gold and silver nanoparticles from Trianthema decandra: Synthesis, characterization, and antimicrobial properties. Int J Nanomedicine 2012;7:5375-84.
53. Namvar F, Rahman HS, Mohamad R, Azizi S, Tahir PM, Chartrand MS, et al. Cytotoxic effects of biosynthesized zinc oxide nanoparticles on murine cell lines. Evid Based Complement Alternat Med 2015;2015:593014.
54. El Kassas HY, Attia AA. Bactericidal application and cytotoxic activity of biosynthesized silver nanoparticles with an extract of the red seaweed Pterocladiella capillacea on the HepG2 cell line. Asian Pac J Cancer Prev 2014;15(3):1299-306.
55. Vaidyanathan R, Kalishwaralal K, Gopalram S, Gurunathan S. Nanosilver â€“ The burgeoning therapeutic molecule and its green synthesis. Biotechnol Adv 2009;27(6):924-37.
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