EVALUATION FOR NEPHROPROTECTIVE ACTIVITY OF ETHANOLIC EXTRACT OF ALLIUM CEPA LINN. IN GENTAMICIN-INDUCED NEPHROTOXICITY IN RATS
Objective: Kidney diseases are a major problem of worldwide proportions, and renal damage is very common since kidney has the capacity to excrete
toxic substances. This study aimed to evaluate the protective effect of the ethanolic extract of Allium cepa Linn. (EEAC) plant leaves against gentamicininduced
nephrotoxicity in rats.
Methods: Nephroprotective activity was estimated by inducing gentamicin (100 mg/kg) to all the groups of animals; acute kidney dysfunction is an
evidenced by significant elevation of serum creatinine, total protein and decreased body weight with multiple histological damages.
Results: Treatment with the A. cepa Linn. has shown significant (p<0.01 and p<0.001) dose-dependent improvement in the body weight at the dose
of 200 and 400 mg/kg and also shown significant improvement by protecting the kidney from the oxidative stress. It is also identified that treatment
with A. cepa significantly lowered the level of serum creatinine, total protein when compared with the toxic group.
Conclusion: Nephroprotective activity of EEAC treatment was found compared with the standard group (Vitamin E â€“ 250 mg/kg) and control group
against the toxic control group animals in parameters including serum creatinine, total protein, kidney weights, and body weights. The histopathological
studies were also evinced the protective effect of EEAC.
Keywords: Nephroprotective activity, Gentamicin, Nephrotoxicity, Allium cepa Linn.
1. Porter GA, Bennett W. Nephrotoxic acute renal failure due to common drugs. Am J Physiol 1981;241(7):252-6.
2. Patel S, Ingalhalli R. Role of plants as nephroprotective agents. Int J Ther Appl 2015;26:6-14.
3. MartÃnez-Salgado C, RodrÃguez-Barbero A, RodrÃguez-Puyol D, PÃ©rez de Lema G, LÃ³pez-Novoa JM. Involvement of phospholipase A2 in gentamicin-induced rat mesangial cell activation. Am J Physiol 1997;273:F60-6.
4. Griffiths G, Trueman L, Crowther T, Thomas B, Smith B. Onions â€“ A global benefit to health. Phytother Res 2002;16(7):603-15.
5. Kyoung AL, Kee-Tae K, Hyun JK, Myong-Soo C, Pahn-Shick C, Hoon P, et al. Antioxidant activities of onion (Allium cepa L.) Peel extracts produced by ethanol, hot water, and subcritical water extraction. Food Sci Biotechnol 2014;23(2):615-21.
6. Geeta BK, Kaksha JP, Bhargav MP, Seema NB, Tripathi CB. Evaluation of cardioprotective effect of aqueous extract of Allium cepa Linn. bulb on isoprenaline-induced myocardial injury in Wistar albino rats. Res Pharm Sci 2016;11(5):419-27.
7. Amit K, Kundan SB, Amteshwar SJ, Richa S. Comparative evaluation of neuroprotective effect of three varieties of Allium cepa in chronic constriction injury induced neuropathic pain. Thai J Pharm Sci 2016;40(1):9-20.
8. Gummalla P, Anusha VL, Hemalatha CH, Anil KY, Sravani K. Anxiolytic and anticonvulsant activity of methanolic extract of Allium cepa Linn (onion) bulbs in Swiss albino mice. J Pharmacogn Phytochem 2015;4(2):131-5.
9. Ozougwu JC. Anti-diabetic effects of Allium cepa (onions) aqueous extracts on alloxan-induced diabetic Rattus novergicus. J Med Plants Res 2011;5(7):1134-9.
10. Teklit GA. In vitro antimicrobial efficacy of fractions from onion (Allium cepa) leaves extract from Wukro, Ethiopia. Am J Life Sci 2015;3(5):365-8.
11. Bibu KJ, Joy AD, Mercey KA. Therapeutic effect of ethanolic extract of Hygrophila spinosa T. Anders on gentamicin-induced nephrotoxicity in rats. Indian J Exp Biol 2010;48(9):911-7.
12. Paoulomi C, Aniruddha M, Subhangkar N. Protective effects of the aqueous leaf extract of Aloe barbadensis on gentamicin and cisplatin-induced nephrotoxic rats. Asian Pac J Trop Biomed 2012;2(3):S1754-63.
13. El-Badwi SM, Bakhiet AO, Abdel EH. Hemato-biochemical effects of aqueous extract of Khaya senegalensis stem bark on gentamicin induced nephrotoxicity in Wistar rats. J Biol Sci 2012;12(6):361-6.
14. Allston CA. In: Anderson SC, Cockayne S, editors. Non Protein Nitrogenous Compounds and Renal Function. Clinical Chemistry: Concepts and Application. Philadelphia, PA, USA: WB Saunders; 1993. p. 369.
15. Vasiliades J. Reaction of alkaline sodium picrate with creatinine: I. Kinetics and mechanism of formation of the mono-creatinine picric acid complex. Clin Chem 1976;22(10):1664-71.
16. Christensen SE. In: Anderson SC, Cockayne S, editors. Proteins, Clinical chemistry: Concepts and Application. Philadelphia, PA, USA: WB Saunders; 1983. p. 188.
17. Tiez NW. Clinical Guide to Laboratory Tests. 3rd ed. Philadelphia, PA, USA: WB Saunders; 1995. p. 518.
18. Karra G, Nadendla R, Sindhu B, Umamaheshwera RV. Nephroprotective, nephrocurative activity of Mimosa pudica root against gentamicin induced nephrotoxicity. Int J Pharm Pharm Sci 2015;7(4):173-7.
19. Vijay KK, Naidu MU, Anwar AS, Ratnakar KS. Probocol protects against gentamicin induced nephrotoxicity in rats. Indian J Pharmacol 2000;32:108-13.
20. Nitha B, Janardhanan KK. Aqueous ethanolic extract of morel mushroom mycelium Morchella esculenta, protects cisplatin and gentamicin induced nephrotoxicity in mice. Food Chem Toxicol 2008;46(9):3193-9.
21. Paoulomi C, Bodhisattawa C, Sudhangkar N. Review on nephroprotective activity study by different plant extract. Adv J Pharm Life Sci Res 2014;2(2):24-40.
22. Afeefa T, Uzma S, Saeed M, Furqan KH, Khalid H, Nadeem IB, et al. Evaluation of protective and curative role of Î±-lipoic acid and selenium in gentamicin induced nephrotoxicity in rabbits. Pak J Pharm Sci 2012;25:103-10.
23. Konda VR, Madhavi E, Lakshmipathy PR, Maignana KR. Antioxidant and nephroprotective activities of Aconitum heterophyllum root in glycerol induced acute renal failure in rats. J Clin Diagn Res 2016;10(3):FF01-5.
24. Faouzi D, Salah A, Mohamed RD, Zine K. Effect of the joint supplementation of vitamin C and vitamin E on nickel heamatotoxicity and nephrotoxicity in male swiss albino mice. Int J Pharm and Pharm Sci 2016;8(6):234-9.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.