PREVALENCE OF VARIOUS BETA LACTAMASES AMONG GRAM NEGATIVE BACILLI IN URINARY ISOLATES FROM PATIENTS IN A TERTIARY CARE HOSPITAL OF NORTHERN INDIA


Rameshwari Thakur, Kumar Atul, Chaudhary Varsha, Singh Paramjit, Sharma Vk, Nidhi Agarwal

Abstract


Objective: Urinary tract infections are considered among the most common infections, occurring either in the community or health-care setting. We are left with very few options for the treatment due to rapid development of antibiotic resistance among the organisms. To find out the prevalence of various types of β-lactamases among urinary isolates.

Methods: Seven antibiotic discs (HiMedia) were placed in combinations and approximation in a particular sequence on a 90 mm diameter MuellerHinton
agar plate.

Results: Out of a total 165 urinary isolates, 66 (40%) isolates were positive for extended spectrum β-lactamase (ESBL) production, AmpC β-lactamases
(AmpC) activity was present in 31 (18.78%) isolates, co-production of both ESBL and AmpC was seen in 16 (9.69%) isolates, 3 (1.81%) isolates
produced metallo β-lactamase (MBL), 2 (1.21%) isolates produced both MBL, and ESBL and 1 (0.60%) isolates were positive for inducible third generation cephalosporin resistance.
Conclusion: With the presence of such high prevalence of various β-lactamases in clinical isolates of gram-negative bacilli and also other types of
antibiotic resistance, antibiotic policy should be made, and strict adherence should be followed.

Keywords: Extended spectrum β-lactamase, AmpC β-lactamase, Metallo β-lactamase.


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References


REFERENCES

Nicolle L. Complicated urinary tract infections in adults. Can J Infect Dis Med Microbiol. 2005; 16:349-60.

Knothe H, Shah P, Krcmery V, Antal M, Mitsuhashi S. Transferable resistance to cefotaxime, cefoxitin, cefamandole and cefuroxime in clinical isolates of Klebsiella pneumoniae and Serratia marcescens. Infection 1983; 11:315-7.

Bauernfeind A, Chong Y, Schweighart S. Extended broad- spectrum ß-lactamase in Klebsiella pneumoniae including resistance to cephamycins. Infection 1989; 17:316-21.

Jarlier V, Nicolas M H, Fournier G, Philippon A. Extended broad-spectrum V, Nicolas M H, Fournier G, Philippon A. Extended spectrum β-lactamases conferring transferrable resistance to newer β-lactam agents in enterobacteriaceae: Hospital prevalence and susceptibility patterns. Rev Infect Dis. 1998; 10: 867-78.

Phillipon A, Alert G, Jacoby GA. Plasmid mediated AmpC β-lactamases. Antimicro Agents Chemother 2002; 46:1-11.

Anananthan S, Subha A. Cefoxitin resistance mediated by a loss of a porin in clinical strains of Klebsiella pneumoniae and E. coli. Indian J Med Microbio. 2005; 23: 20-3.

Fukuda H, Hiramatsu K. Mechanism of endogenous drug resistance acquisition by spontaneous chromosomal gene mutation. Nippon Rusho. 1997; 55: 1185-1190.

Black JA, Moland ES, Thompson KS. AmpC disk test for detection of plasmid mediated AmpC β-lactamases in Enterobacteriaceae lacking chromosomal AmpC β-lactamases. J Clin Microbiol. 2005; 43:3110-3113.

CLSI (2016) Performance Standards for Antimicrobial Susceptibility 26th Edition. M100S (ISBN 1-56238-923-8[Print]; ISBN1-56238-924-6 [Electronic]) Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087 USA.

Tzouvelekis LS, Vatopoulos AC, Katsanis G, Tzelepi E. Rare case of failure by an automated system to detect extended-spectrum beta lactamase in a cephalosporin-resistant Klebsiella pneumoniae isolate [Letter]. J Clin Microbiol 1999; 37:2388.

Tan YT, Ng LSY, He J, Koh Th, Hsu LY. Evaluation of Screening Methods to Detect Plasmid-Mediated AmpC in Escherichia coli, Klebsiella pneumonia, and Proteus mirabilis. Antimicrobl Agents Chemother. 2009; 53:146-149.

Qin X, Weissman SJ, Chestnut MF, ZhangB, Shen L. Kirby-Bauer disc approximation to detect inducible third-generation cephalosporin resistance in Enterobacteriaceae. Ann Clin Microbiol Antimicrob 2004; 3:13

Pitout JD, Gregson DB, Poirel L, McClure JA, Le P, Church DL. The detection of Pseudomonas aeruginosa which produced metallo-β-lactamases in a large centralized laboratory. J Clin Microbiol. 2005; 43:3129-35

Thomson KS, Moland ES, Sanders CC. Use of microdilution panels with and without β-lactamase inhibitors as a phenotype test for β-lactamase production amon Escherichia coli, Klebsiella spp., Enterobacter spp., Citrobacter freundii and Serratia marcescens. Antimicrob Agents Chemother 1999; 43:1393-400.

Nagedo NV, Kaore NM, Thombre. Phenotypic methods for detection of various β-lactamases in Gram-negative clinical isolates: need of hour. Chron Young Sci 2012; 3:292-98.

Oberoi L, Singh N, Sharma P, Aggarwal A. ESBl, MBL and AmpC β Lactamases Producing Superbugs- Havoc in the Intensive Care Units of Punjab India. J Clin Dia Re 2013; 7(1): 70-3.

Haider M, Rizvi M, Fatima N, Shukla I, Malik. Necessity of detection of extended spectrum beta-lactamase, Amp C and metallo-beta-lactamases in Gram-negative bacteria isolated from clinical specimens. Muller J Med Sci Res 2014; 23-8.

Kolhapure RM, Kumar A, Rajkumar HRV. Co-expression of ESBL, AmpC and MBL in Gram-negative bacilli. Int J Res Med Sci 2015; 2698-703.

Amit, A., Rangari, Sharma S, Tyagi N, Singh P, Singh G, Thakur R. Antibiotic Susceptibility Pattern of Bacterial Uropathogens Isolated from Patients at a Tertiary Care Hospital in Western Uttar Pradesh of India. Int. J Curr Microbiol App Sci 2015; 4(10): 646–657.

Livermore DM, β-lactamases in laboratory and clinical resistance. Clin Microbiol Rev. 1995; 8:557-84.

Thomson KS, Moland ES. Cefepime, piperacillin-tazobactam, and the inoculum effect in tests with extended-spectrum-lactamase-producing Enterobacteriaceae. Antimicrob Agents Chemother 2001; 45: 3548-54.

Zanetti G, Bally F, Gerub G, Gabrino J, Kinge T, Lew D, et al. Cefepime versus imipenem-cilastin for treatment of nosocomial pneumonia in intensive care unit patients: a multicentre, evaluator-blind, prospective, randomized study. Antimicrob Agents Chemother 2003;47: 3442-3447.




About this article

Title

PREVALENCE OF VARIOUS BETA LACTAMASES AMONG GRAM NEGATIVE BACILLI IN URINARY ISOLATES FROM PATIENTS IN A TERTIARY CARE HOSPITAL OF NORTHERN INDIA

DOI

10.22159/ajpcr.2017.v10i4.16291

Date

01-04-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 4 April 2017 Page: 129-132

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

45 Views | 62 Downloads

Authors & Affiliations

Rameshwari Thakur
Professor Department of Microbiology Muzaffarnagar Medical College Muzaffarnagar- 241203, UP, India
India

Kumar Atul

Chaudhary Varsha

Singh Paramjit

Sharma Vk

Nidhi Agarwal


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