REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF DICYCLOMINE HYDROCHLORIDE, PARACETAMOL AND MEFENAMIC ACID IN BULK AND TABLET DOSAGE FORM


Janhavi R Rao, Esha Khadge, Savita Yadav

Abstract


ABSTRACT
Objective: The objective of this study was to develop and validate a simple, precise and accurate reverse phase high performance liquid chromatographic
method for simultaneous estimation of dicyclomine hydrochloride, paracetamol, and mefenamic acid from multicomponent tablet dosage form.
Methods: The optimized chromatographic separation was achieved using a stationary phase of hypersil gold ODS- C18 (250 × 4.6 mm i.d, 5 μm
particle size) column and mobile phase of acetonitrile: Methanol: Potassium dihydrogen phosphate buffer, pH adjusted to 4.5 with orthophosphoric
acid (OPA) in the ratio of 50: 30: 20 (v/v/v) with a flow rate of 1 mL/minute. The ultraviolet detection was carried out at 260 nm.
Results: The retention times of paracetamol, dicyclomine hydrochloride and MEF were found to be 4.2, 6.1 and 3.4 minutes, respectively. The method
was validated for linearity, precision, accuracy, and robustness. The developed method provided linear responses within the concentration ranges
2-12 μg/mL for dicyclomine hydrochloride, 50-300 μg/mL for paracetamol, and 25-150 μg/mL for MEF. The % recovery obtained were found to be
99.09-99.44% for dicyclomine hydrochloride, 99.22-99.51% for paracetamol, and 99.49-99.60 for MEF.
Conclusion: The proposed method enables rapid quantification and simultaneous analysis of all the drugs from commercial formulations without any
interference of excipients. Hence, the developed method can be successfully applied for routine analysis of dicyclomine hydrochloride, paracetamol,
and MEF in their combined tablet formulation.
Keywords: Dicyclomine hydrochloride, Paracetamol, Mefenamic acid, High performance liquid chromatography


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About this article

Title

REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF DICYCLOMINE HYDROCHLORIDE, PARACETAMOL AND MEFENAMIC ACID IN BULK AND TABLET DOSAGE FORM

DOI

10.22159/ajpcr.2017.v10i3.16351

Date

01-03-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 3 March 2017 Page: 393-397

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

198 Views | 183 Downloads

Authors & Affiliations

Janhavi R Rao

Esha Khadge

Savita Yadav


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