CURCUMINOID AND TOXICITY LEVELS OF ETHANOL EXTRACT OF JAVANESE GINGER (Curcuma xanthorriza) ON BRINE SHRIMP (Artemia salina) LARVAE AND ZEBRAFISH (Danio rerio) EMBRYOS


Gustini Syahbirin, Nurfadilawati Mumuh, Kusdiantoro Mohamad

Abstract


Objective: This study was aimed at determining the levels of curcuminoids and analyzing the toxicity of ethanol extracts of Javanese ginger.

Methods: Curcuminoid levels were determined using high-performance liquid chromatography, while the toxicity tests were done on larva of brine shrimp (Artemia salina) by using a brine shrimp lethality test (BSLT) method and embryos of zebrafish (Danio rerio) using a zebrafish embryo acute toxicity (ZFET) method.

 

Results: The level curcuminoid of ethanol extracts was 10.5% dry wt., consisting of curcumin at the highest percentage (68.06%) followed by
desmethoxycurcumin (24.6%) and bisdemethoxycurcumin (1.41%). In BSLT method, the lethal concentration 50% values (LC) value of our ethanol extract was 238 ppm, whereas in ZFET method, the LC value at 96 hours after fertilization was 80 ppm. The ethanol extract of ginger caused major malformations of the pericardial edema of zebrafish embryos at a concentration of 100 ppm. 50

Conclusion: The ethanol extract of Curcuma xanthorrhiza from Bogor contained curcuminoids consisting of curcumin, desmethoxycurcumin, and bisdemethoxycurcumin, with acute toxicity, caused major malformations on the pericardial edema in zebrafish embryos.

Keywords: Curcuma xanthorrhiza, Curcuminoid, Toxicity, Zebrafish embryo acute toxicity.50


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About this article

Title

CURCUMINOID AND TOXICITY LEVELS OF ETHANOL EXTRACT OF JAVANESE GINGER (Curcuma xanthorriza) ON BRINE SHRIMP (Artemia salina) LARVAE AND ZEBRAFISH (Danio rerio) EMBRYOS

DOI

10.22159/ajpcr.2017.v10i4.16429

Date

01-04-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 4 April 2017 Page: 169-173

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

112 Views | 186 Downloads

Authors & Affiliations

Gustini Syahbirin
Bogor Agricultural University, Indonesia
Indonesia

Nurfadilawati Mumuh
Bogor Agricultural University, Indonesia
Indonesia

Kusdiantoro Mohamad
Bogor Agricultural University, Indonesia
Indonesia


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