• Mamta Sharma Shoolini University, HP, India
  • Amita Kumari
  • Eshita Mahant


Objectives : Plantago ovata is an important medicinal plant of Himalayan region greatly used in herbal dugs manufacturing. The plant is multipurpose and strictly present in the Himalaya. Plantago has many medicinal properties such as antioxidant, anti-inflammatory and hematopoiesis effects and protects the liver and is used for the treatment of cancer. The plant being medicinal possesses complex phytochemicals. The investigation of various Plantago organ (leaves, stem etc) revealed their high potential to produce a wide array of bioactive secondary metabolites. In present study the a new method of micropropagation through tissue culture  was developed for Plantago so as to meet the future demand of plant. Futher a morphological and physiochemical comparison of tissue culture grown plant was done with in vivo grown plants.

Methods:  Plantago ovata was grown in -vitro through tissue culture technique using MS media and in-vivo in the nursery area of Shoolini University. In vitro culture of  Plantago ovata forsk. were managed to restrict the ecological factors and to control the culture conditions. Experimental culture parameter including germination and phytochemical constituents of Plantago ovata in vivo and in vitro conditions were observed.

Results: The result revealed changes in the concentration of phytochemical constituent's in tissue culture grown Plantago. Phytochemicals constituents (carbohydrate, tannin, chlorophyll, saponin) was reduced in tissue culture grown plant where as some phytochemicals (phenol, alkaloid, flavanoid, protein, phytosterol) increased in tissue culture grown plant than in vivo plant.  A reduction in morphological trait was found in tissue cultured plant.

Conclusion: The developed tissue culture method for the micropropagation of  Plantago ovata can be used as milestone to meet the industrial need in near future.

Keywords: Plantago, Tissue Culture Technique, germination, phytochemicals.

Author Biography

Mamta Sharma, Shoolini University, HP, India


1. Abbas JA, El-Oqlah AA, Mahaneh AM. Herbal plants in the traditional medicine of Bahrain. Economic Botany. 1992; 46: 158-163.
2. Agnihotri K, Ansari SA. Adventitious rhizogenesis in relation to seasonal variation, size of culm branch cuttings and IAA treatment in bamboos. Indian Forester. 2000; 126:971–984.

3. Agnihotri, RK & Nandi, SK. In vitro shoot cut: A high frequency multiplication and rooting method in the bamboo Dendrocalamus hamiltonii. Biotechnology. 2009; 8: 259-263.

4. Anderson, JW, Allgood, LD & Turner, J. Effects of psyllium on glucose and serum lipid responses in men with type 2 diabetes and hypercholesterolemia. American Journal of Clinical Nutrition. 1999; 70:466-47.

5. Begum S, Hassan SI, Siddiqui BS, Shaheen F, Ghayur MN, et al. Triterpenoids from the leaves of Psidium guajava. Phytochemistry. 2002 ; 61: 399-403.

6. BeMiller JN, Whistler RL, Barkalow DG. Aloe, Chia, Flaxseed, Okra, Psyllium seed, Quince seed and Tamarind Gums, In Industrial Gums; polysaccharides and their derivatives (Eds.Whistler R.L. and BeMiller James N.) Academic Press, New York, USA. 1993; 239-244.
7. Bentley R , Trimen H. Medicinal Plants (J & A Churchill, New Burlington Street, London, London, UK, 1880) Allied Book Centre, Dehra Dun, India. 1981; 3: 211.
8. Bräutigam M. & G. Franz. Versuche zur Gewebekultur von schleimbildenden pflanzlichen Geweben. Science pharmaceutica. 1985; 53:237-246.

9. Budzianowska A, L Skrzypczak & J Budzianowski. Phenylethanoid glucosides from in vitro propagated plants and callus cultures of Plantago lanceolata. Plants Medicine. 2004; 70, 834-840.

10. Caccioni DRL, Tonini G, Guizzardi M In vitro antifungal activity of some South African medicinal plants. South African Journal of Botany. 2002; 68: 72-76.

11. Ceranic, M, Kecmanovic, D, Pavlov, M, Sepetkovski, A, Kovacevic, P, Stamenkovic, A, Masirevic, V & Rankovic, V, Plantago ovata. Acta chirurgica iugoslavica. 2006; 53(1):9-11.

12. Che J, M Li, Z Jin - Current research and development of plant polyphenols as skin care factors in cosmetics. Heilongjiang Yiyao. 2006; 19:97-99.

13. Cristoni A, P Morazzoni, - Botanicals for innovative cosme-nutraceuticals. Journal of Applied Cosmetology. 2003; 21: 9-22.

14. Deters AM, KR Schroeder, Smiatek T Hensel A. Plantago ovata seed husk polysaccharides promote proliferation of human epithelial cells (skin keratinocytes and fibroblasts) via enhanced growth factor receptors and energy production. Plant Medicine. 2005; 71: 33-39.

15. Dhar, MK, Kaul, S, Sareen, S, Koul, AK Plantago ovata: Genetic diversity, cultivation, utilization and chemistry. Plant Genetic Resources. 2005; 3:252–263.

16. Dijkstra P & PJC Kuiper - Effects of exogenously applied growth regulators on shoot growth of inbred lines of Plantago major differing in relative growth rate: differential response to gibberellic acid and (2- chloroethyl)-trimethyl-ammonium chloride. Physiology Plant. 1989; 77:512-518.

17. Dijkstra P, H Ter Reegen & PJC Kuiper. Relation between relative growth rate, endogenous gibberellins, and the response to applied gibberellic acid for Plantago major. Physiology Plant. 1990; 79:629-634.

18. Fischer, HM, Nanxiong, Y Gray, GR, Ralph, GJ, Anderson, L and Marlett, JA. The gel-forming polysaccharide of psyllium husk (Plantago ovata Forsk). Carbohydrate Resseach. 2004; 339:2009–2017.

19. Fons F., A. Gargadennec, A. Gueiffier, J.L. Roussel & C. Andary.- Effects of cinnamic acid on polyphenol production in Plantago lanceolata. Phytochemistry. 1998; 49:697-702.

20. Fons F, D Tousch, S Rapior, A Gueiffier, JL Roussel, A Gargadennec & C Andary,.- Phenolic profiles of untransformed and hairy root cultures of Plantago lanceolata. Plant Physiology Biochemistry. 1999; 37:291-296.

21. Han GY, Wang XF, Zhang GY, Ma ZY Somatic embryogenesis and plant regeneration of recalcitrant cottons (Gossypium hirsutum). African journal of Biotechnology. 2009; 8(3):432-437.

22. Jamal, S, Ahmad, Y, Agarwal, R, Ahmad, M and Osman, SM A novel oxo fatty acid in Plantago ovata seed oil. Phytochemical. 1987; 26:3067–3069.

23. Jasrai YT, N Yadava & AR Mehta- Somatic embryogenesis from leaf induced cell cultures of Plantago ovata Forsk. J Herbs, Spices & Medicinal Plants, 1, 11-16. Journal of Applied Sciences Research. 1993; 7(3): 327-332.

24. Kalyansundram, NK, Patel, PB & Dalal, KC Nitrogen need of Plantago ovata Forsk. in relation to the available nitrogen in the soil. Indian Journal of Agriculture Science. 1982; 52:240–242.

25. Kalyansundram, NK, Sriram, S, Patel, BR, Patel, DH, Dalal, KC and Gupta, R Psyllium: a monopoly of Gujarat. Indian Journal of Horticulture. 1984; 28:35–37.

26. Karimzadeh, G & Omidbaigi, R Growth and seed characterization of Isabgol (Plantago ovata Forsk) as influenced by some environmental factors. Journal of Agricultural Science and Technology. 2004; 6:103-10.

27. Khawar KM, Sarhin EO, Sevimay CS, Cocu S, Parmaksiz I, Uranbey S, Ipek A, Kaya MD, Sancak C & S Ozcan, - Adventitious shoot regeneration and micropropagation of Plantago lanceolata L. Periodicum Biologorum. 2005; 107:113-116.

28. Patel, RB, Rana, NG & Patel, MR Chromatographic screening of proteins of Plantago ovata Forsk. Indian Journal of Pharmaceutical Science. 1979; 41: 249-253.

29. Pramanik S, S Chakraborty & S Sen Raychaudhuri. In vitro clonal propagation and characterization of clonal regenerants of Plantago ovata Forsk. By isozyme analysis. Cytobios. 1995; 82:123-130.

30. Rahn, K, A phylogenetic study of the Plantaginaceae. Botanical Journal of the Linnean Society. 1996; 120:145 198.

31. Zafar R, Aeri V, Datta A. Application of plant tissue and cell culture for production of secondary metabolites. Fitoterapia. 1992; 63:33-43.
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How to Cite
Sharma, M., A. Kumari, and E. Mahant. “MICROPROPOGATION AND ANALYSIS OF PHYTOCHEMICAL PROFILE OF TISSUE CULTURE GROWN PLANTAGO OVATA FORSK.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 4, Apr. 2017, pp. 202-6, doi:10.22159/ajpcr.2017.v10i4.16532.
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