PHYTOCHEMICAL STUDIES AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF SARCOSTEMMA BREVISTIGMA, WIGHT & ARN.
Objective: To explore the phytochemical constituents present in Sarcostemma brevistigma using gas chromatography-mass spectrometry (GC-MS).
Methods: Preliminary phytochemical analysis of secondary metabolites was made by following standard procedures. GC-MS analysis of the plant
extracts was performed by using GC-MS equipped with a DB-35MS capillary standard non-polar column and gas chromatograph interfaced to a Mass
Selective Detector (MS-DSQ-II) with Xcalibur software.
Results: The qualitative phytochemical analysis of this species exhibited the presence of alkaloids, glycosides, flavonoids, phenols, steroids, amino
acids, tannins, terpenoids, quinones, and coumarin. The GC-MS analysis revealed the presence of 24 compounds in the ethanolic extract of aerial part
of Sarcostemma brevistigma. The most prevailing compound was 14,17 bis(dimethylaminoamino)[3.3]paracyclophan-5,8-diacetonitrile (14.86%).
Conclusion: The study concludes that the species Sarcostemma brevistigma is a potential source for bioactive compounds such as esters, alkanes,
alcohols, alkenes, amide, amine, phenol, ketone, and so forth. This study justifies the traditional usage of this species.
Keywords: Gas chromatography-mass spectrometry, Sarcostemma brevistigma, Phytochemical compounds, Medicinal plants.
1. Home Page on the Internet, World Health Organization. Available from: http://www.who.int/medicines/areas/traditional/definitions/en/.
2. Nakahara K, Roy MK, Alzoreky NS, Thalang V, Trakoontivakorn G. Inventory of Indigenous Plants and Minor Crops in Thailand Based on Bioactivities: 9th JIRCAS. International Symposium-Value Addition to Agricultural Product; 2001. p. 135-9.
3. Karuppasamy B, Antony N, Veerabahu RM. GC-MS analysis of Polycarpaea corymbosa (L.) Lam. whole plant. Asian Pac J Trop Biomed 2012;2(3):1289-92.
4. Gopalakrishnan S. GC-MS analysis of some bioactive constituents of Mussaenda frondosa Linn. Int J Pharm Biosci 2011;2(1):313-20.
5. Janakiraman N, Johnson M, Sathish SS. GC-MS analysis of bioactive constituents of Peristrophe bicalyculata (Rets.) Nees. (Acanthaceae). Asian Pac J Trop Biomed 2012;46-9.
6. Chopra RN, Chopra IC, Handa KL, Kapoor LD. Indigenous Drugs of India. Calcutta, India: Academic Publishers; 1958.
7. Nadkarni AK. Indian Material Medica, Popular. Mumbai: Prakashan Pvt., Ltd.; 1982.
8. Poornima N, Umarrajan KM, Babu K. Studies on anatomical and phytochemical analysis of Oxystelma esculentum (L.f) R. Br. Ex Schltes. Int J Bot Res 2009;2(4):239-43.
9. Prajapati ND, Purohit SS, Sharma AK, Kumar TA. Hand Book of Medicinal Plants. India: Agrobios; 2003.
10. Kirtikar KR, Basu BD. Indian Medicinal Plants. Dehradun, India: International Book Distributors; 1976.
11. Dahiya K, Verma M, Dhankhar R, Singh V, Ghalaut PS, Seth S. Alteration of ischemia modified albumin and nitric oxide levels in hypothyroidism. Clin Lab 2014;60(6):969-72.
12. Elgorashi EE, Van Staden J. Pharmacological screening of six Amaryllidaceae species. J Ethnopharmacol 2004;90(1):27-32.
13. Vishnu R, Nisha R, Jamuna S, Paulsamy S. Quantification of total phenolics and flavonoids and evaluation of in vitro antioxidant properties of methanolic leaf extract of Tarenna asiatica - An endemic medicinal plant species of Maruthamali hills. Western Ghats, Tami Nadu. J Res Plant Sci 2013;2(2):196-204.
14. Benedec D, Vlase L, Oniga I, Mot AC, Damian G, Hanganu D, et al. Polyphenolic composition, antioxidant and antibacterial activities for two Romanian subspecies of Achillea distans Waldst. et Kit. ex Wild. Molecules 2013;18(8):8725-39.
15. Gbadamosi IT, Moody JO, Lawal AM. Phytochemical screening and proximate analysis of eight ethno botanicals used as antimalaria remedies in Ibadan, Nigeria. J Appl Biosci 2011;44:2967-71.
16. Bisht R, Chanyal S, Agrawal PK. Antimicrobial and phytochemical analysis of leaf extract of medicinal fruit plants. Asian J Pharm Clinical Res 2016;9(4):131-6.
17. Porwal V, Singh P, Gurjar DA. Comprehensive study on different methods of extraction from Guajava leaves for curing various health problems. Int J Eng Res 2012;2(6):490-6.
18. Sathyaprabha G, Kumaravel S, Ruffina D, Praveenkumar PA. Comparative study on antioxidant, proximate analysis, antimicrobial activity and phytochemical analysis of Aloe and Cissus quadrangularis by GC-MS. J Pharm Res 2010;3:2970-3.
19. Ngane AN, Biyiti L, Bouchet PH, Nkegfact A, Zolo PH. Antifungal activity of Piper guineense of Cameroun. Fitoterapia 2003;4(5):464-8.
20. Nwinyi OC, Chinedu NS, Ajani OO. Evaluation of antibacterial activity of Pisidium guajava and Gongronema latifolium. J Med Plants Res 2008;2(8):189-92.
21. Nwaichi EO, Igbinobara O. Effect of some selected spices on some biochemical profile of wistar Albino rats. Am J Environ Engine 2012;2(1):8-11.
22. Kumar A, Rani S, Niketa SS. Recent review on plant molecular biology, phytophysiology, phytochemistry and ethnopharmacology of Cuscuta reflexa Roxb. A wonderful parasitic plant. Int Res J Pharm 2012;3(7):30-8.
23. Belkacem N, Djaziri R, Lahfa F, El-Haci IA, Boucherit Z. Phytochemical screening and in vitro antioxidant activity isolated bioactive compounds from Tridax procumbens Linn. Paki J Biol Sci 2013;16(24):1971-7.
24. Jegadeeswari P, Nishanthini A, Muthukumarasamy S, Mohan VR. GC-MS analysis of bioactive components of Aristolochia krysagathra (Aristolochiaceae). J Curr Chem Pharm Sci 2012;2(4):226-32.
25. Antara S, Amla B. Chemical composition of methanolic extract of the leaves of Melia azedarach L. Asian J Pharm Clinical Res 2012;5(3):42-5.
26. Prabu MK, Sanydurai P, Subbaiyan B, Thangapandian V. Phytochemical constituentsand gas chromatography - Mass spectrometry analysis of Caralluma diffusa, (Wight) N.E.BR. Aerial part. Int J Pharm Pharm Sci 2013;5(3):602-5.
27. Blenkharn JI. The antimicrobial activity of taurolin - A possible additive for parenteral nutrition solutions. Clin Nutr 1987;6(1):35-8.
28. Sowmya S, Perumal PC, Gopalakrishnan VK. Chromatographic and spectrophotometric analysis of bioactive compounds from Cayratia trifolia (L.) stem. Int J Pharm Pharm Sci 2016;8(6):57-64.
29. Saradha M, Paulsamy S. GC-MS analysis for bioactive compounds from methanolic leaf and stem bark extracts of Hildegardia populifolia (Roxb.) Schott and Endl. Int J Pharm Sci Rev Res 2013;23(2):328-32.
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