EVALUATION OF ANTIHYPERLIPIDEMIC ACTIVITY OF LEAVES OF ACHYRANTHES ASPERA Linn USING HYPERLIPIDEMIC RATS


Sarvesh Cn, Jennifer Fernandes

Abstract


Objective: Plants have been one of the important sources of medicines since the beginning of human civilization. Achyranthes aspera Linn. belonging
to family Amaranthaceae, commonly found in India. The plant is used as antiarthritic, purgative, diuretic, antimalarial, etc.

Methods: The antihyperlipidemic activity of alcoholic and aqueous extracts of leaves of A. aspera was evaluated using triton-induced hyperlipidemia
model in albino Wistar rats. The acute toxicity study confirmed that both the extracts were found to be safe at 5000 mg/kg b.w. in female albino
Wistar rats. In this model, eight groups (six animals per group) of animals were used. Group I and II were considered as saline and hyperlipidemic
controls, respectively, and other groups received their respective agents (alcoholic or aqueous extract/atorvastatin/ayurvedic preparation) and all the
groups received triton WR1339 (200 mg/kg b.w. i.v.) except saline control group. Serum cholesterol and triglycerides (TGs) level are estimated for the
evaluation of antihyperlipidemic effects of extracts.

Results: The ethanolic extract has shown a significant reduction in serum cholesterol and TGs level indicating antihyperlipidemic potentials in the
plant while aqueous extract has not shown any beneficial effect.
Conclusion: In this triton-induced hyperlipidemia model, serum and TG levels were analyzed ethanolic extract was shown antihyperlipidemic activity.
Further clinical studies are compulsory to confirm the findings.

Keywords: Achyranthes aspera, High-density lipoprotein, Low-density lipoprotein, Triglyceride, Total cholesterol, Triton, Antihyperlipidemic.


| PDF |

References


Goyal BR, Goyal RK, Mehta AA. Phyto-pharmacology of Achyranthes aspera Linn: A review. Pharmacogn Rev 2007;1(1):143-50.

Ballantyne CM, Keefe JH, Gotto AM. Dyslipidemia Essentials Physicians. New York, New Delhi: Jaypee Press; 2003. p. 3-4, 60-74, 128.

Anand M, Selvaraj V, Alagar M. Phytochemical screening and evaluation of (in vitro) antioxidant activity of Achyranthes aspera Linn. root extract. Int J Pharm Pharm Sci 2014;6(2):197-9.

Harborne JB. Phytochemical Methods. London: Chapmann and Hall; 1973. p. 89.

Mazumber A, Basu SP, Saha BP, Mazumder R. Phytochemical observation on leaf of lagerstromia parviflora (Roxb). Anc Sci Life 2002;22(2):21-4.

Ghosh MN. Fundamentals of Experimental Pharmacology. 3rd ed. Kolkata: Hilton & Company; 2005. p. 190-6.

OECD. Guidelines for the Testing of Chemicals. Acute Oral Toxicity - Up-and-Down-Procedure (UDP). OECD 425. Paris: Organization for Economic Cooperation and Development; 2006.

Akhtar MS, Iqbal J. Evaluation of the hypoglycaemic effect of Achyranthes aspera in normal and alloxan-diabetic rabbits. J Ethnopharmacol 1991;31(1):49-57.

Vogel HG, Vogel WH, Scholkens BA, Sandew J, Miller G, Vogel WF. Drug Discovery and Evaluation. 2nd ed. Berlin, New York: Springer, Verlag; 2002. p. 1103-6.

Schurr PE, Schultz JR, Parkinson TM. Triton induced hyperlipidemia in rats as an animal model for screening hypolipidemic drugs. Lipids 1971;7(1):68-73.

Buccolo G, David H. Quantitative determination of serum triglycerides by the use of enzymes. Clin Chem 1973;19(5):476-82.

Werner M, Gabrielson DG, Eastman J. Ultramicro determination of serum triglycerides by bioluminescent assay. Clin Chem 1981;27(21):268-71.

Annoni GG, Bottasso RM, Ciaci D, Donato MF, Tripoli A. Clinical investigation of triglycerides. J Res Lab Med 1982;9:115.

Allain CC, Poon LS, Chan CS, Richmond W, Fu PC. Enzymatic determination of total serum cholesterol. Clin Chem 1974;20(4):470-5.

Kumar V, Khan MM, Khanna AK, Singh R, Singh S, Chander R, et al. Lipid lowering activity of Anthocephalus indicus root in hyperlipidemic rats. Evid Based Complement Alternat Med 2010;7(3):317-22.

Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972;18(6):499-502.

Al-Zubairi A, Al-Habori M, Al-Geiry A. Effect of Catha edulis (khat) chewing on plasma lipid peroxidation. J Ethnopharmacol 2003;87(1):3-9.

Holmgren PR, Brown AC. Serum cholesterol levels of nondiabetic and streptozotocin-diabetic rats fed a high cholesterol diet. Artery 1993;20(6):337-45.

Khanna AK, Chander R, Singh C, Srivastava AK, Kapoor NK. Hypolipidemic activity of Achyranthus aspera Linn in normal and triton induced hyperlipemic rats. Indian J Exp Biol 1992;30(2):128-30.

Rohit KV, Thomas P. Herbal medicines used in the traditional Indian medicinal system as a therapeutic treatment option for overweight and obesity management: A review. Int J Pharm Pharm Sci 2014;6(2):40-7.




About this article

Title

EVALUATION OF ANTIHYPERLIPIDEMIC ACTIVITY OF LEAVES OF ACHYRANTHES ASPERA Linn USING HYPERLIPIDEMIC RATS

DOI

10.22159/ajpcr.2017.v10i4.16576

Date

01-04-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 4 April 2017 Page: 211-215

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

93 Views | 135 Downloads

Authors & Affiliations

Sarvesh Cn
Mallige College of Pharmacy, Bengaluru, Karnataka, India - 560 090
India

Jennifer Fernandes


Article Tools


Email this article (Login required)
Email the author (Login required)

Refbacks

  • There are currently no refbacks.