FABRICATION OF NOVEL ANTICANCER POLYOXOMETALATE [CoW11O39(CpTi)]7- -CHITOSAN NANO-COMPOSITE, ITS TOXICITY REDUCTION AND SUSTAINED RELEASE


Vamangi M Pandya, Sachin A Joshi

Abstract


Objectives: Polyoxometalates (POMs) are proved to be important for applications in medicine and in material science. Here, we represent
nanocomposite formation of tungsten-containing potent anticancer polyanion, K 6 H [CoW 11 O 39  (CpTi)].13H 2 O (CoW CpTi) with biocompatible ChitosanYC-100 (CSYC100) with the goal to reduce its heavy metal toxicity.

Methods: Synthesis of “POM-CSYC100 nanocomposite” was attained without the aid of any cross-linker through electrostatic interaction technique. Nanocomposites were characterized using Fourier transform infrared spectroscopy, dynamic light scattering, transmission electron microscopy, and thermogravimetric analysis. The release profile recorded was slow and sustained at physiological pH. In vitro cytotoxicity assays which show an attribute to reduce the toxicity of these POM were performed on C2C12 (mouse myoblast cell line) and A-549 (lung cancer cell line), which proved the reduced toxicity of nanocomposites as compared to the bare drugs.

Results: Sustained release studies showed there was a slow and steady release of CoW CpTi for 11 hrs, with the 98% of collective release at the end. From in vitro cytotoxic assay, it was deduced that CoW 11 11 CpTi -CSYC100 nanocomposite at the concentrations of 1.25 mM, and lower did not exhibit toxic effect on C2C12 cells as 95% total C2C12 cell mass remained viable. While in the case of A549 cells highest 5 mM concentration of bare CoW 11 CpTi is toxic to the cancer cells and after encapsulation cell viability increases from 10% to 55%.

Conclusion: Thus, this study has designated the probability of using POM-chitosan nanocomposite for less toxic and effective biomedicinal applications.
Keywords: Anticancer, Chitosan, Nanocomposite, In vitro cytotoxicity, Drug release.


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About this article

Title

FABRICATION OF NOVEL ANTICANCER POLYOXOMETALATE [CoW11O39(CpTi)]7- -CHITOSAN NANO-COMPOSITE, ITS TOXICITY REDUCTION AND SUSTAINED RELEASE

DOI

10.22159/ajpcr.2017.v10i4.16721

Date

01-04-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 4 April 2017 Page: 278-282

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

89 Views | 57 Downloads

Authors & Affiliations

Vamangi M Pandya
Dr. K. C. Patel R & D Center, Charotar University of Science and Technology (CHARUSAT), Changa-388421 Dist. Anand. Gujarat. India

Sachin A Joshi
Dr. K. C. Patel R & D Center, Charotar University of Science and Technology (CHARUSAT), Changa-388421 Dist. Anand. Gujarat. India
India


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