• Subhash T Kumbhar CRD, PRIST University, Thanjavur, TN, India & Indira College of Pharmacy, Pune, MS, India
  • Hemant D Une YB Chavan College of Pharmacy, Roza Baug, Harsul Road, Aurangabad. http://orcid.org/0000-0002-6854-6611
  • Dayanand M Kannur Indira College of Pharmacy, Pune
  • Shriniwas P Patil Indira College of Pharmacy, Pune


Objective: This study was designed to evaluate the protective effect of Canna indica L. extract against caffeine-nicotine administration-induced type 2 diabetes exaggeration in rats.

Methods: A study was conducted for three weeks in four rat groups (n=6); viz.  type 2 diabetic control group, a caffeine-nicotine diabetic control group (20mg/kg, 0.4mg/kg, ip twice daily),  and Canna indica L. extract and caffeine-nicotine treatment group and  standard drug treated caffeine-nicotine diabetic group (Glibencamide, 5mg/kg, once daily). Type 2 diabetes was induced by two weeks high fatty diet and a single dose streptozotocin (50mg/kg, ip) on 1th day of the study in all groups. Blood and urine samples were collected every week for serum biochemical analysis.

Results: Results of extract treatment and standard drug treatment were compared with untreated caffeine-nicotine co-administration group. Difference in each relevant serum parameter was analyzed through ANOVA and Dunett’s t test. Extract treated caffeine-nicotine-diabetic group showed about 150-200mg/dL (p<0.001) reduction in the serum glucose than untreated caffeine-nicotine-diabetic control group. Extract treatment reduces serum glucose by 10-15 mg/dL than glibenclamide treatment with higher significance (p<0.001). Extract treatment showed better results than standard drug in liver and kidney function test and exhibited its better potential in controlling diabetic complications. Extract treatment increased HDL-C and reduced triglycerides, LDL-C, VLDL-C and TC much better and with higher significance than standard drug. Extract treatment reduced TC by at least 60-80mg/dL (p<0.01) in comparison to caffeine-nicotine-diabetic control group. Extract treatment reduced 10-15mg/dL of more total cholesterol than that of standard drug.

Conclusion: Caffeine-nicotine co-administration-induced exaggeration of type 2 diabetes was better treated by CI extract than that of standard drug gibenclamide. 

Keywords: Type 2 diabetes, Streptozotocin, Caffeine, Nicotine, Diabetic complication, Rat

Author Biographies

Subhash T Kumbhar, CRD, PRIST University, Thanjavur, TN, India & Indira College of Pharmacy, Pune, MS, India
Hemant D Une, YB Chavan College of Pharmacy, Roza Baug, Harsul Road, Aurangabad.
Dayanand M Kannur, Indira College of Pharmacy, Pune
Shriniwas P Patil, Indira College of Pharmacy, Pune


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How to Cite
Kumbhar, S. T., H. D. Une, D. M. Kannur, and S. P. Patil. “EFFECT OF CANNA INDICA L. EXTRACT AGAINST CAFFEINE-NICOTINE CO-ADMINISTRATION-INDUCED EXAGGERATION IN TYPE 2 DIABETIC RATS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 4, Apr. 2017, pp. 347-51, doi:10.22159/ajpcr.2017.v10i4.16845.
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