• KUMBHAR SUBHASH T CRD, PRIST University, Thanjavur, TN, India &Indira College of Pharmacy, Pune, MS, India
  • UNE HEMANT D YB Chavan College of Pharmacy, Roza Baug, Harsul Road, Aurangabad. http://orcid.org/0000-0002-6854-6611
  • KANNUR DAYANAND M Indira College of Pharmacy, Pune
  • PATIL SHRINIWAS P Indira College of Pharmacy, Pune


Objective: This study was designed to evaluate the protective effect of Canna indica L. extract against caffeine-nicotine administration-induced type 2 diabetes exaggeration in rats.

Methods: A study was conducted for three weeks in four rat groups (n=6); viz.  type 2 diabetic control group, a caffeine-nicotine diabetic control group (20mg/kg, 0.4mg/kg, ip twice daily),  and Canna indica L. extract and caffeine-nicotine treatment group and  standard drug treated caffeine-nicotine diabetic group (Glibencamide, 5mg/kg, once daily). Type 2 diabetes was induced by two weeks high fatty diet and a single dose streptozotocin (50mg/kg, ip) on 1th day of the study in all groups. Blood and urine samples were collected every week for serum biochemical analysis.

Results: Results of extract treatment and standard drug treatment were compared with untreated caffeine-nicotine co-administration group. Difference in each relevant serum parameter was analyzed through ANOVA and Dunett's t test. Extract treated caffeine-nicotine-diabetic group showed about 150-200mg/dL (p<0.001) reduction in the serum glucose than untreated caffeine-nicotine-diabetic control group. Extract treatment reduces serum glucose by 10-15 mg/dL than glibenclamide treatment with higher significance (p<0.001). Extract treatment showed better results than standard drug in liver and kidney function test and exhibited its better potential in controlling diabetic complications. Extract treatment increased HDL-C and reduced triglycerides, LDL-C, VLDL-C and TC much better and with higher significance than standard drug. Extract treatment reduced TC by at least 60-80mg/dL (p<0.01) in comparison to caffeine-nicotine-diabetic control group. Extract treatment reduced 10-15mg/dL of more total cholesterol than that of standard drug.

Conclusion: Caffeine-nicotine co-administration-induced exaggeration of type 2 diabetes was better treated by CI extract than that of standard drug gibenclamide. 

Keywords: Type 2 diabetes, Streptozotocin, Caffeine, Nicotine, Diabetic complication, Rat

Author Biographies

KUMBHAR SUBHASH T, CRD, PRIST University, Thanjavur, TN, India &Indira College of Pharmacy, Pune, MS, India


UNE HEMANT D, YB Chavan College of Pharmacy, Roza Baug, Harsul Road, Aurangabad.


KANNUR DAYANAND M, Indira College of Pharmacy, Pune


PATIL SHRINIWAS P, Indira College of Pharmacy, Pune



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How to Cite
SUBHASH T, K., U. HEMANT D, K. DAYANAND M, and P. SHRINIWAS P. “EFFECT OF CANNA INDICA L. EXTRACT AGAINST CAFFEINE-NICOTINE CO-ADMINISTRATION-INDUCED EXAGGERATION IN TYPE 2 DIABETIC RATS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 4, Apr. 2017, pp. 347-51, doi:10.22159/ajpcr.2017.v10i4.16845.
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