MOLECULAR CHARACTERIZATION AND DRUG RESISTANCE OF ENTEROPATHOGENIC BACTERIA ISOLATED FROM READY TO EAT FOOD IN LUDHIANA

  • Naresh Kumar Department of Biotechnology, Maharaj Vinayak Global University Jaipur, Rajasthan, India
  • Jangir Op Department of Biotechnology, Maharaj Vinayak Global University Jaipur, Rajasthan, India

Abstract

Objective: The study was conducted to investigate the enteropathogenic bacteria from the most common street food sold in Ludhiana at different
places.

Methods: A total of 90 samples of street food samples were randomly collected from four locations of Ludhiana. Samples investigated for the presence of bacteria using appropriate selective and differential culture media. The spread plate technique was used to analyze the samples.

Results: A total of 90 samples were analyzed, and the total viable counts of the bacterial population in all food samples were in the range of
2.0 × 103 to 1.8 × 106 CFU/g. The nonvegetarian food samples recorded the 1.8 × 106 CFU/g; however, other street foods also have highest total viable
microbial count 1.1 × 106 CFU/g. The total coliform count ranged from 1.9 × 103 to 9.4 × 105 CFU/g, and pani-puri has significant count. The range of
Staphylococci count was 1.9 × 103 to 5.3 × 103 CFU/g, chicken tandoori sample also high count bacteria. The incidence of manifestations of the seven
pathogenic bacteria isolated from all street food Proteus sp. (2%) Staphylococci were the most predominant. This was followed by Salmonella (6%), Staphylococcus aureus (14%), Klebsiella sp. (5%), and Enterococcus sp. (2%) were least predominant.

Conclusion: The study exhibited contagion may be due to poor techniques of food preparation adopted by the street food vendors. This is of community health worry as these bacteria are known as food-borne illnesses and toxic syndrome.

 

Keywords: Pathogenic organisms, Ready-to-eat, Food, Toxic syndrome, Ludhiana.

Author Biography

Naresh Kumar, Department of Biotechnology, Maharaj Vinayak Global University Jaipur, Rajasthan, India
Medical Sciences

References

1. Muzaffar AT, Huq I, Mallik BA. Entrepreneurs of the streets: An analytical work on the street food vendors of Dhaka city. Int J Bus Manag 2009;4(2):80-8.
2. Zeru K, Kumie A. Sanitary conditions of food establishments in Mekelle town, Tigray, North Ethiopia. Ethiop J Health Dev 2007;21(1):3-11.
3. Andargie G, Kassu A, Moges F, Tiruneh M, Huruy K. Prevalence of bacteria and intestinal parasites among food-handlers in Gondar town, Northwest Ethiopia. J Health Popul Nutr 2008;26(4):451-5.
4. Manu DY, Darko KO, Ablordey A, Mensah P. Street foods in Accra, Ghana: How safe are they? Bull World Health Organ 2002;80(7):546-54.
5. Dias MT, Bricio SM, Almeida DO. Molecular characterization and evaluation of antimicrobial susceptibility of enter pathogenic
E. coli (EPEC) isolated from minas soft cheese. Ciênc Tecnol Aliment 2012;32(4):747-53.
6. Nicolas B, Abdoul RB, Aly S, Amadou TO, Jules IA, Alfred ST. Hygienic status assessment of dish washing waters, utensils, hands and pieces of money from street food processing sites in Ouagadougou (Burkina Faso). Afr J Biotechnol 2006;5(11):1107-12.
7. Cheesbrough M. Every effort has Reproduced from Basic Laboratory Procedures in Clinical Bacteriology. 2nd ed. Geneva: World Health Organization Laboratory Should Issue Written Instructions to All; 2000, 2003, 2006.
8. World Health Organization. Manual of Basic Techniques for a Health Laboratory. 2nd ed. Geneva: World Health Organization; 2003.
9. Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966;45:493-6.
10. Rindhe SN, Zanjad PN, Doifode VK, Siddique A, Mendhe MS. Assessment of microbial contamination of chicken products sold in Parbhani city. Vet World 2008;1(7):208-10.
11. Chumber SK, Kaushik K, Savy S. Bacteriological analysis of street foods in Pune. Indian J Public Health 2007;51(2):114-6.
12. Ates M, Ozkizilcik A, Tabakoglu C. Microbiological analysis of stuffed mussels sold in the streets. Indian J Microbiol 2011;51(3):350-4.
13. Ike CC, Emeka-Ike PC, Nwokorie CC, Anochie CC. Microbiological quality evaluation of locally prepared snacks sold in Aba Metropolis, Abia State, Nigeria. Int J Sci Eng Appl Sci IJSEAS 2015;1(7):46-59.
14. Monday IE, Francis JI, Mohammad SU. Microbiological quality of ready-to-eat foods (Rice and Moimoi) sold by food vendors in federal polytechnic Bali, Taraba State Nigeria. IOSR J Environ Sci Toxicol Food Technol IOSR JESTFT 2014;8(2):145-9.
15. Odu NN, Akano UM. The microbiological assessment of ready-to-eatfood (Shawarma) in Port Harcourt city, Nigeria. Nat Sci 2012;10(8):1-8.
16. Most Food, Water Samples Fail Purity Test - Ludhiana Tribune 22nd June, 2015. Available from: http://www.indiaonlinepages.com/ population/punjab-population.html.
17. Tambekar DH, Kulkarni RV, Shirsat SD, Bhadange DG. Bacteriological quality of street vended food panipuri: A case study of Amravati City (Ms) India. Biosci Discov 2011;2(3):350-4.
18. Estrada-Garcia T, Lopez-Saucedo C, Zamarripa-Ayala B, Thompson MR, Gutierrez-Cogco L, Mancera-Martinez A, et al. Prevalence of Escherichia coli and Salmonella spp. In street-vended
food of open markets (tianguis) and general hygienic and trading
Statistics
414 Views | 564 Downloads
Citatons
How to Cite
Kumar, N., and J. Op. “MOLECULAR CHARACTERIZATION AND DRUG RESISTANCE OF ENTEROPATHOGENIC BACTERIA ISOLATED FROM READY TO EAT FOOD IN LUDHIANA”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 5, May 2017, pp. 131-4, doi:10.22159/ajpcr.2017.v10i5.17918.
Section
Original Article(s)