GREEN SYNTHESIS OF SILVER NANOPARTICLES: ITS EFFECT ON QUORUM SENSING INHIBITION OF URINARY TRACT INFECTION PATHOGENS
Objective: The present study was conducted to evaluate the antimicrobial and anti-quorum sensing (QS) ability of nanoparticles synthesized using
Methods: QS is a system of response and stimuli correlated to population density. Bacteria by QS secrete certain signaling molecules called autoinducers. These bacteria also have a receptor that can specifically detect the signaling molecule known as an inducer. Since QS governs numerous processes in bacteria including virulence, QS inhibition promises to be an ideal target for the development of novel therapeutics. In the present study, silver nanoparticles (AgNPs) synthesized from the ginger, lemon peel, cinnamon, corn silk, pomegranate peel, and orange peel exhibited anti-QS properties by inhibiting violacein production in Chromobacterium violaceum assayed using agar well diffusion method.
Results: AgNPs were synthesized from various cost-effective fruit waste sources. These AgNPs exhibited significant antibacterial and anti-QS properties.
Conclusion: Hence, such sources can be explored for developing the effective therapy for urinary tract infections.
2. Flemming HC, Wingender J. The biofilm matrix. Nat Rev Microbiol 2010;8(9):623-33.
3. Miller MB, Bassler BL. Quorum sensing in bacteria. Annu Rev Microbiol 2001;55:165-99.
4. Blosser RS, Gray KM. Extraction of violacein from Chromobacterium violaceum provides a new quantitative bioassay for N-acyl homoserine lactone autoinducers. J Microbiol Methods 2000;40(1):47-55.
5. Gala V, Desai K. Plant based quorum sensing inhibitors of Pseudomonas aeruginosa. Int J Pharm Pharm Sci 2014;(6)8:20-5.
6. Koh CL, Sam CK, Yin WF, Tan LY, Krishnan T, Chong YM, et al. Plant-derived natural products as sources of anti-quorum sensing compounds. Sensors 2013;13(5):6217-28.
7. Bhardwaj AK, Vinothkumar K, Rajpara N. Bacterial quorum sensing inhibitors: Attractive alternatives for control of infectious pathogens showing multiple drug resistance. Recent Pat Antiinfect Drug Discov
8. Al-Hussaini R, Mahasneh AM. Microbial growth and quorum sensing antagonist activities of herbal plants extracts. Molecules 2009;14(9):3425-35.
9. Mary RN, Banu N. Screening of antibiofilm and anti-quorum sensing potential of Vitex trifolia in Pseudomonas aeruginosa. Int J Pharm Pharm Sci 2015;7(8):242-24.
10. Markowska K, Grudniak AM, Wolska KI. Silver nanoparticles asan alternative strategy against bacterial biofilms. Acta Biochim Pol 2013;60(4):523-30.
11. Christensen GD, Simpson WA, Bisno AL, Beachey EH. Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces. Infect Immun 1982;37(1):318-26.
12. Mathur T, Singhal S, Khan S, Upadhyay DJ, Fatma T, Rattan A. Detection of biofilm formation among the clinical isolates of staphylococci: An evaluation of three different screening methods. Indian J Med Microbiol 2006;24(1):25-9.
13. Hartree EF. Determination of protein: A modification of the Lowry method that gives a linear photometric response. Anal Biochem 1972;48(2):422-7.
14. Song JY, Kim BS. Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess Biosyst Eng 2009;32(1):79-84.
15. Hassan A, Usman J, Kaleem F, Omair M, Khalid A, Iqbal M. Evaluation of different detection methods of biofilm formation in the clinical isolates. Braz J Infect Dis 2011;15(4):305-11.
16. Shaw PD, Ping G, Daly SL, Cha C, Cronan JE Jr, Rinehart KL, et al. Detecting and characterizing N-acyl-homoserine lactone signal molecules by thin-layer chromatography. Proc Natl Acad Sci U S A 1997;94(12):6036-41.
17. Khan MS, Zahin M, Hasan S, Husain FM, Ahmad I. Inhibition of quorum sensing regulated bacterial functions by plant essential oils with special reference to clove oil. Lett Appl Microbiol 2009;49(3):354-60.
18. Baqai R, Aziz M, Rasool G. Urinary tract infections in diabetic patients and biofilm formation of uropathogens. Infect Dis J Pak 2008;17(1):21-4.
19. Ronald A. The etiology of urinary tract infection: Traditional and emerging pathogens. Dis Mon 2003;49(2):71-82.
20. Ghosh R, Tiwary BK, Kumar A, Chakraborty R. Guava leaf extract inhibits quorum-sensing and Chromobacterium violaceum induced lysis of human hepatoma cells: Whole transcriptome analysis reveals differential gene expression. PloS One 2014;9(9):e107703.
21. Kahlmeter G; ECO.SENS. An international survey of the antimicrobial susceptibility of pathogens from uncomplicated urinary tract infections: The ECO.SENS Project. J Antimicrob Chemother 2003;51(1):69-76.
22. Packiavathy IA, Priya S, Pandian SK, Ravi AV. Inhibition of biofilm development of uropathogens by curcumin - An anti-quorum sensing agent from Curcuma longa. Food Chem 2014;148:453-60.
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