• VENKATA RAJESH KUMAR T Department of Botany, S. V. University, Tirupati, Andhra Pradesh, India
  • NARAYANA RAO MADAMSETTI Department of Botany, S. V. University, Tirupati, Andhra Pradesh, India
  • Bhargava Y Department of Botany, S. V. University, Tirupati, Andhra Pradesh, India
  • Murthy Jsr Department of Botany, S. V. University, Tirupati, Andhra Pradesh, India


Objective: Synthesis of varied sized and morphologically distinct silver nanoparticles (AgNPs) using callus/callus extract, and their promising antibacterial and cytotoxicity was reported from very few plant systems. Here, we investigated silver nanoparticle synthetic potential of Couroupita guianensis leaf callus extract and their antibacterial activity.

Methods: synthesis of callus mediated silver nanoparticles and characterisation of physical, chemical, and antibacterial activity of AgNPs.

Results: Callus extract rapidly reduced silver ions and stabilized nanoparticles have displayed characteristic maximum UV absorbance at 410 nm. SEM and AFM images revealed their spherical morphology and size variation, which is ranged from 30.38 nm to 88.32 nm and were in small aggregates. Capping of AgNPs by the phenolic compounds and proteins revealed from FTIR spectral peaks. Silver nanoparticles displayed significantly high antimicrobial activity against both Gram positive and negative bacterial strains compared to silver ions and gentamicin. This enhanced antimicrobial activity of AgNPs may due their small size leading to efficient molecular contact with cell surface, and uptake and interaction with vital biomolecules.

Conclusions: Stable AgNPs were synthesized through reduction and capping of silver ions by polyphenols and proteins present in callus extract. The
enhanced antimicrobial activity of AgNPs may due to their small size leading to efficient molecular contact with the cell surface, penetration, and
interaction, and inactivation of vital biomolecules.


Keywords: Couroupita guianensis, callus extract, Silver nanoparticles (AgNPs), antibacterial activity


1. Kumar TV, Murthy JS, Rao MN, Bhargava Y. Evaluation of silver nanoparticles synthetic potential of Couroupita guianensis Aubl., Flower buds extract and their synergistic antibacterial activity.
3 Biotech 2016;92(6):1-9.
2. Moore BD, Andrew RL, Külheim C, Foley WJ. Explaining intraspecific diversity in plant secondary metabolites in an ecological context. New Phytol 2014;201(3):733-50.
3. Naik PM, Al-Khayri JM. Impact of abiotic elicitors on in vitro production of plant secondary metabolites: A review. J Adv Res Biotech 2016;1(2):1-7.
4. Xia QH, Ma YJ, Wang JW. Biosynthesis of silver nanoparticles using Taxus yunnanensis callus and their antibacterial activity and cytotoxicity\ in human cancer cells. Nanomaterials 2016;160(6):1-15.
5. Mitre M. Couroupita guianensis. In: 1998. IUCN 2013. IUCN Red List of Threatened Species. Version 2013.2.
6. Manimegalai S, Rakkimuthu G. Phytochemical screening of stem of Couroupita guianensis. Int J Pharm Sci Res 2012;3(11):4434-7.
7. Wong KC, Tie DY. Volatile constituents of Couroupita guianensis Aubl. Flowers. J Essent Oil Res 1995;7(2):225-7.
8. Rane JB, Vahanwala SJ, Goltkar SG, Ambaye RY, Khadse BG. Chemical examination of the flowers of Couroupita guianensis Aubl. Indian J Pharm Sci 2001;63:72-3.
9. Prabhu V, Subban R. Quantification of quercetin and stigmasterol of Couroupita guianensis Aubl by HPTLC method and in-vitro cytototoxic activity by MTT assay of the methanol extract against HeLa, NIH 3t3 and HEPG2 cancer cell lines. Int J Pharm Pharm Sci 2012;4(4):126-30.
10. Murashige T, Skoog F. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 1962;15(3):473-97.
11. Iyer RI, Selvaraju C, Santhiya ST. Biosynthesis of silver nanoparticles by callus cultures of Vigna radiata. Indian J Sci Technol 2016;9(9):1-5.
12. Lukman AI, Gong B, Marjo CE, Roessner U, Harris AT. Facile synthesis, stabilization, and anti-bacterial performance of discrete Ag nanoparticles using Medicago sativa seed exudates. J Colloid Interface Sci 2011;353(2):433-44.
13. Nabikhan A, Kandasamy K, Raj A, Alikunhi NM. Synthesis of antimicrobial silver nanoparticles by callus and leaf extracts from saltmarsh plant, Sesuvium portulacastrum L. Colloids Surf B
Biointerfaces 2010;79(2):488-93.
14. Satyavani K, Gurudeeban S, Ramanathan T, Balasubramanian T. Biomedical potential of silver nanoparticles synthesized from calli cells of Citrullus colocynthis (L.) Schrad. J Nanobiotechnology
15. Hegazy HS, Rabie GH, Shaaban LD, Raie DS. Extracellular synthesis of silver nanoparticles by callus of Medicago sativa. Life Sci J 2014;11(10):1211-4.
16. Kirthika P, Dheeba B, Sivakumar R, Abdulla SS. Plant mediated synthesis and characterization of silver nanoparticles. Int J Pharm Pharm Sci 2014;6(8):304-10.
17. Mude N, Ingle A, Gade A, Rai M. Synthesis of silver nanoparticles using callus extract of Carica papaya-A first report. J Plant Biochem Biotechnol 2009;18(1):83-6.
18. Shah M, Fawcett D, Sharma S, Tripathy SK, Poinern GE. Green synthesis of metallic nanoparticles via biological entities. Materials 2015;8(11):7278-308.
19. Rai VR, Bai AJ. Nanoparticles and their potential application as antimicrobials. Science Against Microbial Pathogens, Communicating Current Research and Technological Advances. Vol. 1. Badajoz: Formatex; 2011. p. 197-09.
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How to Cite
VENKATA RAJESH KUMAR T, NARAYANA RAO MADAMSETTI, B. Y, and Murthy Jsr. “COUROUPITA GUIANENSIS LEAF CALLUS EXTRACT MEDIATED SYNTHESIS AND ANTIBACTERIAL ACTIVITY OF SILVER NANOPARTICLES.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 5, May 2017, pp. 126-30, doi:10.22159/ajpcr.2017.v10i5.17066.
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