ISOLATION AND CHARACTERIZATION OF POTENTIAL PROBIOTIC YEASTS FROM DIFFERENT SOURCES


Nilanjana Das, Mangala Lakshmi Ragavan

Abstract


objective The main objective of the present study is to isolate yeasts from different environmental sources to investigate their potential probiotic characteristics.

Methods Appropriate in vitro assays has been conducted to examine their probiotic potentiality, such as acid and bile salt tolerance, temperature resistance, stimulated GIT tolerance, cell adhesion and cholesterol removal. All the yeast isolates were tested under in vitro conditions.

Results In the present study, 20 yeast species have been isolated from different sources, screened and their desirable probiotic properties viz. pH tolerance, bile salt tolerance and thermo-tolerance have been evaluated. Screened yeast isolates treated with gastric juices showed increased survival rate above 60%. A further in vitro study investigates cholesterol removal and it showed increased cholesterol removal rate up to 92%. Exopolysaccharide production was estimated for selected yeast isolates and applications are under investigation.

Conclusion Among 20 yeast isolates, 5 isolates showed increased growth under stress tolerance. It can be concluded that the screened yeast isolates LM, MR, GOI, GII2 and WI can serve as promising probiotics in various fields of food industry.

Key words:  Acid tolerance; bile tolerance, cholesterol removal, probiotics, yeast.


| PDF |

References


Joint FAO. WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria. Córdoba, Argentina: October; 2001. p. 1-4.

Hooper LV, Gordon JI. Commensal host-bacterial relationships in the gut. Science 2001;292(5519):1115-1118.

Gorbach SL. Probiotics in the third millennium. Dig Liver Dis 2002;34 Suppl 2:S2-7.

Mainville I, Arcand Y, Farnworth ER. A dynamic model that simulates the human upper gastrointestinal tract for the study of probiotics. Int J Food Microbiol 2005;99(3):287-96.

Botes M. Survival of Probiotic Lactic Acid Bacteria in the Intestinal Tract, Their Adhesion to Epithelial Cells and Their Ability to Compete with Pathogenic Microorganisms, Dissertation. Stellenbosch: Stellenbosch University; 2008.

Patterson JA, Burkholder KM. Application of prebiotics and probiotics in poultry production. Poult Sci 2003;82(4):627-31.

Floch MH, Walker WA, Madsen K, Sanders ME, Macfarlane GT, Flint HJ, et al. Recommendations for probiotic use-2011 update. J Clin Gastroenterol 2011;45 Suppl: S168-71.

Cotter PD, Hill C, Ross RP. Bacteriocins: Developing innate immunity for food. Nat Rev Microbiol 2005;3(10):777-88.

Servin AL. Antagonistic activities of lactobacilli and bifidobacteria against microbial pathogens. FEMS Microbiol Rev 2004;28(4):405-40.

Hudson LE, Fasken MB, McDermott CD, McBride SM, Kuiper EG, Guiliano DB, et al. Functional heterologous protein expression by genetically engineered probiotic yeast Saccharomyces boulardii. PLoS One 2014;9(11):e112660.

Posteraro B, Sanguinetti M, Romano L, Torelli R, Novarese L, Fadda G. Molecular tools for differentiating probiotic and clinical strains of Saccharomyces cerevisiae. Int J Food Microbiol 2005;103(3):295-304.

Maragkoudakis PA, Zoumpopoulou G, Miaris C, Kalantzopoulos G, Pot B, Tsakalidou E. Probiotic potential of Lactobacillus strains isolated from dairy products. int Dairy J 2006;16(3):189-99.

Wang J, Xing Z, Tang W, Zheng Y, Wang Y. Isolation, identification, and potential probiotic characterization of one Lactococcus from kefir grain. Food Sci Biotechnol 2015;24(5):1775-80.

Prabhurajeshwar C, Chandrakanth RK. Development of in vitro methodologies for inhibition of pathogenic bacteria by potential probiotic Lactobacillus sps; An evidence for production of antimicrobial substances. Int J Pharm Pharm Sci 2012;8(12):277-86.

Ayama H, Sumpavapol P, Chanthachum S. Effect of encapsulation of selected probiotic cell on survival in simulated gastrointestinal tract condition. Songklanakarin J Sci Technol 2014;36(3):291-9.

Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966;45(4):493-6.

Hatoum R, Labrie S, Fliss I. Antimicrobial and probiotic properties of yeasts: From fundamental to novel applications. Front Microbiol 2012;3:421.

Ravella SR, Quiñones TS, Retter A, Heiermann M, Amon T, Hobbs PJ. Extracellular polysaccharide (EPS) production by a novel strain of yeast-like fungus Aureobasidium pullulans. Carbohydr Polym 2010;82(3):728-32.

Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem 1956;28(3):350-6.

Dunne C, O’Mahony L, Murphy L, Thornton G, Morrissey D, O’Halloran S, et al. In vitro selection criteria for probiotic bacteria of human origin: Correlation with in vivo findings. Am J Clin Nutr 2001;73 2 Suppl:386S-92.




About this article

Title

ISOLATION AND CHARACTERIZATION OF POTENTIAL PROBIOTIC YEASTS FROM DIFFERENT SOURCES

DOI

10.22159/ajpcr.2017.v10i4.17067

Date

01-04-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 4 April 2017 Page: 451-455

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

79 Views | 134 Downloads

Authors & Affiliations

Nilanjana Das
VIT University, Vellore

Mangala Lakshmi Ragavan


Article Tools


Email this article (Login required)
Email the author (Login required)

Refbacks

  • There are currently no refbacks.