• SHABI RUSKIN R Department of Biotechnology, Noorul Islam College of Arts and Science, Kanyakumari, Tamil Nadu, India.
  • VasanthaKumari B Department of Botany, SreeAyyappa College for Women, Chunkankadai, Tamil Nadu, India.
  • citarasu T Department of Marine Biotechnology, Centre for Marine Science andTechnology, MS University, Rajakkamangalam, Tamil Nadu, India


Objectives: The objective of this study was to estimate the total phenolic and flavonoid contents and to explore the antioxidant potential of various leaf extracts (chloroform, ethyl acetate, and ethanol) of Canthium coromandelicum, which is considered traditionally as an important medicinal plant.

Methods: Antioxidant properties of the extracts were assessed using 1, 1- diphenyl -2- picrylhydrazyl and hydrogen peroxide radical scavenging assays for ascorbic acid equivalents. The total phenolic and flavonoid contents were also investigated to determine their correlation with the antioxidant activity of the leaf extracts and expressed in Gallic acid and quercetin equivalents, respectively.

Results: The results showed that the content of total phenols and flavonoids was found to be high in ethyl acetate extract which was recorded as 61.02±1.30 mg Gallic acid equivalent (GAE/g) and 81.72±0.61 QE/g, respectively. Compared to other extracts, ethyl acetate leaf extract was found to possess high antioxidant activity at p<0.05 level, with high percentage of inhibition at 100 μg/ml concentration (82.70%) toward hydrogen peroxide radical scavenging with IC50 value 62.94 μg/ml. Statistically, two-tailed Pearson's correlation showed strong positive correlations between hydrogen
peroxide radical scavenging activity and total phenolic contents (TPC) (r=1.000) at p<0.05 level.

Conclusion: The results obtained in this study clearly signifies that the ethyl acetate leaf extract of C. coromandelicum has high content of total phenols which are correlated to its antioxidant activity and thus has the potential to use as a source of natural antioxidants and can be explored as a therapeutic agent in free radical induced diseases.


Keywords: Canthium coromandelicum, In-vitro antioxidant activity, Ethyl acetate extract, Pearson’s correlation, 1, 1- diphenyl -2- picrylhydrazyl, Hydrogen peroxide, Nitric oxide, Superoxide.


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How to Cite
SHABI RUSKIN R, V. B, and citarasu T. “IN VITRO ANTIOXIDANT ACTIVITY OF VARIOUS LEAF EXTRACTS OF CANTHIUM COROMANDELICUM(BURM.F.) ALSTON.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 5, May 2017, pp. 214-8, doi:10.22159/ajpcr.2017.v10i5.17221.
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