Method development and Validation of RP-HPLC method for the Determination of Olanzapine in Bulk and Tablet Dosage form

  • SAIBABA SV Department of Pharmacy, Mewar University, Chittorgarh, Rajasthan, India
  • Shanmuga Pandiyan P Department of Pharmacy, Mewar University, Chittorgarh, Rajasthan, India

Abstract

Objective: To develop and validate reverse phase-high performance liquid chromatographic method for estimation of olanzapine in bulk and tablet dosage form.

Methods: Chromatographic analysis was performed on XTerra C18 (150×3.5 mm inner diameter, 5 μm) column using a mobile phase consisting of buffer (potassium dihydrogen phosphate) and methanol (45:55% v/v) with a flow rate of 0.6 ml/minutes. The detection was carried out at 247 nm.

Results: The calibration curve of olanzapine was linear in the range of 30-70 μg/ml. The mean % assay of marketed formulation was found to be 100.2%, and % recovery was observed in the range of 98-102%. Relative standard deviation for the precision study was found <2%.

Conclusion: The developed method is simple, precise and rapid, making it suitable for estimation of olanzapine in bulk and tablet dosage form.

 

Keywords: Olanzapine, Reverse phase-high performance liquid chromatographic, Validation.

References

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SAIBABA SV, and S. P. P. “Method Development and Validation of RP-HPLC Method for the Determination of Olanzapine in Bulk and Tablet Dosage Form”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 5, May 2017, pp. 281-4, doi:10.22159/ajpcr.2017.v10i5.17375.
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