ANALYSIS OF GUT FLORA FROM DAMP WOOD TERMITES (TRINERVITERMES SPP.) AND EXTRACTION, CHARACTERIZATION OF CELLULASE FROM THE ISOLATE


G Narendrakumar, Saikrishna Nmd, Prakash P, Preethi Tv

Abstract


Objective: The objective of the study was to isolate, characterize, identify, and compare the potentials of cellulolytic strains isolated from the gut of damp wood termites (Trinervitermes species) collected from Chennai, Tamil Nadu, India.

Methods: Termites were collected and used as hoard of cellulase producers and screened for the cellulase producers using carboxymethyl cellulose as the sole source of carbon and were authenticated using Congo red plate screening method. The isolates showing a significant zone of clearance were further confirmed by biochemically characterized.

Results: Nine effective isolates were characterized and three strained were used for analysis. The organisms were subjected with substrate, temperature (25°C, 37°C, and 45°C), and pH to optimize the cultural condition. The enzyme activity was estimated using endoglucanase, FPase after incubating at appropriate conditions. Five isolates showing a significant zone of clearance were selected, out of which three belonged to Bacillus and one each to Staphylococcus. Optimization of media and genetic modification of the strains can further convalesce their competence. All the isolates have ensuring application in view of use in future.

Conclusion: An effective strain of bacteria was isolated from the gut of termites can be used as a potential candidate for the production of cellulase in industries.


Keywords


Trinervitermes, Cellulase, Lignocellulose material.

| PDF |

References


Wood TG, Johnson RA. The biology, physiology, and ecology of termites. In: Vinson SB, editors. Economic Impact and Control of Social Insects. New York: Praegar; 1986. p. 1-68.

Brauman A, Bignell DE, Tayasu I. Soil–feeding termites: Biology, microbial associations and digestive mechanisms In: Abe T, Bignell DE, Higashi M, editors. Termites: Evolution, Sociality, Symbiosis, Ecology. Dordrecht: Kluwer Academic Publishers; 2000. p. 233-59.

Brune A. Termite guts: The world’s smallest bioreactors. Trends Biotechnol 1998;16:16-21.

Bignell DE, Oskarsson H, Anderson JM. Distribution and abundance of bacteria in the gut of a soil–feeding termite Procubitermes aburiensis (Termitidae, Termitinae). J Gen Microbiol 1980;117:393-403.

Collins NM, Wood TG. Termites and atmospheric gas production. Science 1984;224(4644):84-6.

Collins NM. The utilization of nitrogen resources by termites (Isoptera). In: Lee JA, McNeill S, Rorison IC, editors. Nitrogen as an Ecological Factor. Oxford: Blackwell Scientific Publications; 1983. p. 381-412.

Claus D, Berkeley RC. Genus Bacillus Cohn. In: Sneath PH, editor. Bergey’s Manual of Systematic Bacteriology. Vol. 2. Baltimore, MD: Williams and Wilkins Co.; 1986. p. 1105-39.

Lee KE, Wood TG. Termites and Soils. New York: Academic Press; 1971.

Williams ST, Sharpe ME. Bergey’s Manual of Systematic Bacteriology. Vol. 4. Baltimore, MD: Williams and Williams; 1989.

Cappuccino JG, Sherman N. Microbiology. A Laboratory Manual. 6th ed. San Francisco, California: Pearson Education Inc.; 2002. p. 215-24.

Brauman A, Doré J, Eggleton P, Bignell D, Breznak JA, Kane MD. Molecular phylogenetic profiling of prokaryotic communities in guts of termites with different feeding habits. FEMS Microbiol Ecol 2001;35(1):27-36.

Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press; 1989.

Utharalakshmi N, Kumar AG, Narendrakumar G. Degradation analysis of different agro-substrates by Aspergillus flavus SB04 using FT-IR. Res J Pharm Biol Chem Sci 2016;7(4):105-12.

Fall S, Hawelin J, Ndiaye F, Assigbetse K, Aragno M, Chotte JL, Brauman A. Differences between bacterial communities in the gut of a soil feeding termite (Cubitermes niokoloensis) and its mound. Appl Environ Microbiol 2007;73(16):5199-208.

Holt JA, Lepage M. Termites and soil properties. In: Abe T, Bignell DE, Higashi M, editors. Termites Evolution, Sociality, Symbioses, Ecology. Vol. 1. Dordrecht, Netherlands: Kluwer Academic; 2000. p. 389-407.

Kappler A, Brune, A. Influence of gut alkalinity and oxygen status on mobilization and size–class distribution of humic acids in the hindgut of soil–feeding termites. Appl Soil Ecol 1999;13:219-29.

Mezes PS, Lampen JO. Secretion of proteins by Bacillus 151-185. In: Dubnau DA, editors. The Molecular Biology of the Bacillus. Vol. 2. New York: Academic Press; 1985.

Boga HI, Ji R, Ludwig RW, Brune A. Sporotalea propionica genera novel species. Hydrogen oxidizing, oxygen reducing, propiogenic firmicutes from the intestinal tract of a soil feeding termite. Arch Microbiol 2007;187(1):15-27.

Leadbetter JR, Breznak JA. Physiological ecology of Methanobrevibacter cuticularis species novel and Methanobrevibacter curvatus species novel isolated from the hindgut of the termite Reticulitermes flavipes. Appl Environ Microbiol 1996;62:3620-31.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193(1):265-75.

Rouland C, Brauman A, Labat M, Lepage M. Nutritional factors affecting methane emission from termites. Chemosphere 1993;26:617-22.

Schmitt–Wagner D, Friedrich MW, Wagner B, Brune A. Phylogenetic diversity, abundance, and axial distribution of bacteria in the intestinal tract of two soil–feeding termites (Cubitermes species). Appl Environ Microbiol 2003;69:6007-17.

Prabu D, Narendrakumar G. Optimization of process parameters using response surface methodology for removal of phenol by nano zero valent iron impregnated cashew nut shell. Int J Pharm Pharm Sci 2015;7(4):156-61.

Narendrakumar G, Kumar S, Vimalan S, Prakash P, Nandagopal S, Kumar RB. Optimization of growth promoters on Desmodium gangeticum (L) DC using RSM CCD and its antioxidants activity. Int J Pharm Pharm Sci 2014;6(8):503-7.

Tayasu I, Sugimoto A, Wada E, Abe T. Xylophagous termites depending

on atmospheric nitrogen. Naturwissenschaften 1994;81:229-31.

Utharalakshmi N, Kumar AG, Narendrakumar G. Optimization of cellulase producing Aspergillus flavus SB4 by solid state fermentation using rice bran. J Pure Appl Microbiol 2014;8(2):713-9.

Utharalakshmi N, Kumar AG, Narendrakumar G. Optimization of cellulase producing Aspergillus flavus SB4 by solid state fermentation using response surface methodology (RSM)-CCD. Res J Pharm Technol 2015;4(8):349-54.




About this article

Title

ANALYSIS OF GUT FLORA FROM DAMP WOOD TERMITES (TRINERVITERMES SPP.) AND EXTRACTION, CHARACTERIZATION OF CELLULASE FROM THE ISOLATE

Keywords

Trinervitermes, Cellulase, Lignocellulose material.

DOI

10.22159/ajpcr.2017.v10i6.17565

Date

01-06-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 6 June 2017 Page: 233-236

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

116 Views | 62 Downloads

Authors & Affiliations

G Narendrakumar
Department of Biotechnology, School of Bio and Chemical Engineering, Sathyabama University, Chennai - 600 119, Tamil Nadu, India
India

Saikrishna Nmd
Department of Biotechnology, School of Bio and Chemical Engineering, Sathyabama University, Chennai - 600 119, Tamil Nadu, India
India

Prakash P
Department of Biotechnology, School of Bio and Chemical Engineering, Sathyabama University, Chennai - 600 119, Tamil Nadu, India
India

Preethi Tv
Department of Microbiology, School of Life Science, Vels University, Chennai - 600 117, Tamil Nadu, India
India


Article Tools


Email this article (Login required)
Email the author (Login required)

Refbacks

  • There are currently no refbacks.