ANTIOXIDANT POTENTIAL PROFILE OF PAJANELIA LONGIFOLIA (WILLD.) K. SCHUMAN.; POTENTIAL NEW SOURCES OF NATURAL ANTIOXIDANT.


Priyanka Saha, Anupam Das Talukdar, Manabendra Dutta Choudhury

Abstract


Objectives: The aim of the present work is to screen the antioxidant potentiality of the bark extracts of Pajanelia longifolia (Willd.) K. Schuman, ethnomedicinally prescribed plant as hepatoprotective.

Methods: Bark extract was prepared using Soxhlet apparatus. Total phenol, flavonoid, total alkaloid were analyzed using Folin–Ciocalteu assay, aluminum chloride calorimetric assay, atropine standard, respectively. Antioxidant capacity and free radical scavenging potentialities were done by various in vitro methods, viz.; 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide scavenging activity, reducing power assay, metal chelating activity, ferric reducing antioxidant power (FRAP) assay, and thiobarbituric acid (TBA) method.

Result: Total phenol, flavonoid, and alkaloid were found to be highest in acetone extract of P. longifolia (Willd.) K. Schuman. Acetone extracts of the bark of P. longifolia (Willd.) K. Schuman showed very low inhibitory concentration 50 (IC50) and effective concentration 50 values for DPPH assay and reducing power assay compared to other extracts. In hydrogen peroxide scavenging activity assay, the lowest IC50 value was recorded in acetone extract with 181.78±0.09 μg/ml in P. longifolia. The FRAP assay for P. longifolia acetone extract showed the highest activity at 967±0.69 μg/ml of ascorbic acid equivalent. The ferric reducing capacity and TBA values of the plant extracts confirmed the presence antioxidant principles in the bark of the said plant.

Conclusion: These underused plants may be used for mitigating the detrimental effect of oxidative stress and reactive oxygen species-mediated disease and thus justifies its use in folklore medicines.

 


Keywords


Antioxidant, Free radical, Oxidative stress, Phenols, Flavonoids, Alkaloids, Pajanelia longifolia (Willd.) K. Schuman.

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References


Zainab A, Bhat RP, Acharya S, Yende A, Prajna PS, Padyana S. Studies on antioxidant and antimicrobial activities of Pajanelia longifolia (Willd.) Schumann. J Res Obes 2013;1-12. Available from: http://www.ibimapublishing.com/articles/OBES/2013/756484/756484.pdf.

Chin YW, Balunas MJ, Chai HB, Kinghorn AD. Drug discovery from natural sources. AAPS J 2006;8(2):E239-53.

Choudhury S, Choudhury MD, Sharma GD, Paul SB. Antibacterial activity of crude leaf extracts of Pajanelia longifolia (Wild.) K Schuman. Assam Univ J Sci Technol 2010;5:53-7. Available from: http://www.inflibnet.ac.in/ojs/index.php/AUJSAT/article/view/61/58.

Sharma P, Mazumder S, Choudhury S. Preliminary phytochemical screening and anti-bacterial activity of the leaves of Pajanelia longifolia (Wild.) K Schuman and Crataeva magna (Lour.) DC. Indo Am J Pharm Res 2013;3:8165-8. Available from: http://www.ejmanager.com/mnstemps/36/36-1393662065.pdf.

Datta S, Choudhury S, Choudhury MD. Hepatoprotective activity of bark extracts of Pajanelia longifolia (Willd.) K. Schuman against CCl4 induced hepatic damage in mice. Drug Invent Today 2012;4:537-9. Available from: http://www.jprsolutions.info/newfiles/journal-file-55efb235ce8b09.72866367.pdf.

Kameswaramma A, Seshadri TR. Proc Indian Acad Sci Math Sci 1947;25:43. Available from: http://www.link.springer.com/article/10.1007/BF03172544.

Slinkard K, Singleton VL. Total phenol analyses: Automation and comparison with manual methods. Am J Enol Vitic 1977;28:49-55. Available from: http://www.ajevonline.org/content/28/1/49.short.

Arvouet-Grand A, Vennat B, Pourrat A, Legret P. Standardization of propolis extract and identification of principal constituents. J Pharm Belg 1994;49:462-8.

Ajanal M, Gundkalle MB, Nayak SU. Estimation of total alkaloid in Chitrakadivati by UV-Spectrophotometer. Anc Sci Life 2012;31(4):198-201.

Kumarasamy Y, Byres M, Cox PJ, Jaspars M, Nahar L, Sarker SD. Screening seeds of some Scottish plants for free radical scavenging activity. Phytother Res 2007;21(7):615-21.

Oyaizu M. Antioxidative activities of browning reaction prepared from glucosamine. Jpn J Nutr 1986;44:307-15. Available from: https://www.jstage.jst.go.jp/article/eiyogakuzashi1941/44/6/44_6_307/_article.

Jayaprakasha GK, Jaganmohan Rao L, Sakariah KK. Antioxidant activities of flavidin in different in vitro model systems. Bioorg Med Chem 2004;12(9):5141-6.

Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Anal Biochem 1996;239:70-6. Available from: http://www.sciencedirect.com/science/article/pii/S0003269796902924.

Yan LY, Teng LT, Jhi TJ. Antioxidant properties of guava fruits: Comparison with some local fruits. Sunway Acad J 2006;3:9-20.

Kikuzaki H, Nakatani N. Antioxidant effects of some ginger constituents. J Food Sci 1993;58:1407-10. Available from: http://www.onlinelibrary.wiley.com/doi/10.1111/j.1365-h21.1993.tb06194.x/pdf.

Gheldof N, Engeseth NJ. Antioxidant capacity of honeys from various floral sources based on the determination of oxygen radical absorbance capacity and inhibition of in vitro lipoprotein oxidation in human serum samples. J Agric Food Chem 2002;50:3050-55.

Holasova M, Fiedlerova V, Smrcinova H, Orsak M, Lachman J, Vavreinova S. Buckwheat-the source of antioxidant activity in functional foods. Food Res Int 2002;35:207-211. Available from: http://www.sciencedirect.com/science/article/pii/S0963996901001855.

Aljadi AM, Kamaruddin MY. Evaluation of the phenolic contents and antioxidant capacities of two Malaysian floral honeys. Food Chem 2004;85:513-8. Available from: http://www.sciencedirect.com/science/article/pii/S0308814602005964.

Song FL, Gan RY, Zhang Y, Xiao Q, Kuang L, Li HB. Total phenolic contents and antioxidant capacities of selected Chinese medicinal plants. Int J Mol Sci 2010;11(6):2362-72.

Kumar VS, Kumar GS, Sivaramakrishnan S, Sujatha K, Razia M. Evaluation of phytoconstituents, in vitro antioxidant and antimicrobial activities of edible white button mushroom Agaricus bisporus. Asian J Pharm Clin Res 2016;8:67-71. Available from: http://innovareacademics.in/journals/index.php/ijpps/article/view/8616.

Meziti A, Bouriche H, Hiche M, Kada S, Senator A, Dimertas I. Antioxidant and anti-inflammatory activities of Rubus fruticosus and Zizyphus vulgaris methanol extracts. Int J Pharm Pharm Sci 2017;9:69-76. Available from: http://www.innovareacademics.in/journals/index.php/ijpps/article/view/14374.

Parameshwar P, Reddy YN. Protective role, in vitro free radical scavenging activities of Alangium salvifolium (linn) against CCl4 induced hepatic damage in rats. Int J Pharm Pharm Sci 2015;7:447-52. Available from: http://www.innovareacademics.in/journals/index.php/ijpps/article/view/4480.

Erol N, Sarı F, Velioglu Y. Polyphenols, alkaloids and antioxidant activity of different grades Turkish black tea. GIDA 2010;35:161-8. Available from: https://www.researchgate.net/publication/255649072_Polyphenols_alkaloids_and_antioxidant_activity_of_different_grades_turkish_black_tea.

Racková L, Májeková M, Kost’álová D, Stefek M. Antiradical and antioxidant activities of alkaloids isolated from Mahonia aquifolium. Structural aspects. Bioorg Med Chem 2004;12(17):4709-15.




About this article

Title

ANTIOXIDANT POTENTIAL PROFILE OF PAJANELIA LONGIFOLIA (WILLD.) K. SCHUMAN.; POTENTIAL NEW SOURCES OF NATURAL ANTIOXIDANT.

Keywords

Antioxidant, Free radical, Oxidative stress, Phenols, Flavonoids, Alkaloids, Pajanelia longifolia (Willd.) K. Schuman.

DOI

10.22159/ajpcr.2017.v10i6.17750

Date

01-06-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 6 June 2017 Page: 184-188

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

90 Views | 76 Downloads

Authors & Affiliations

Priyanka Saha
Department of Life Science & Bioinformatics, Ethnobotany & Medicinal Plant Research Laboratory, Assam University, Silchar - 788 011,
India

Anupam Das Talukdar
Department of Life Science & Bioinformatics, Ethnobotany & Medicinal Plant Research Laboratory, Assam University, Silchar - 788 011,
India

Manabendra Dutta Choudhury
Department of Life Science & Bioinformatics, Ethnobotany & Medicinal Plant Research Laboratory, Assam University, Silchar - 788 011,
India


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