María Cristina Furrianca, Marysol Alvear, Tomás Zambrano, Víctor Fajardo, Luis Salazar


Objective: To objective of this work was to perform phytochemical qualitative and quantitative analyzes of the main secondary metabolites in the root of Berberis microphylla.

Methods: The extracts of B. microphylla root were tested through phytochemical screening and the quantification of the most important constituents was carried out using spectrophotometric and gravimetric techniques.

Results: Phytochemical screening of both extracts showed the presence of alkaloids, flavonoids, glycosides, cardiac glycosides, saponins, terpenes,

and tannins, which are pharmacologically important. Quantification of the major phytochemicals groups showed that the ethanolic extract contains 3.9% alkaloids, 0.46% flavonoids, 9.53% tannins, and 3.60% saponins. Similarly, the methanolic extract contains 6.61% alkaloids, 0.41% flavonoids, 7.40% tannins, and 1.43% saponins.

Conclusion: This is the first time that the presence of tannins, flavonoids, and saponins in this plant has been reported. The medicinal properties of the root of B. microphylla may exist due to the presence secondary metabolites.


Calafate, Berberis microphylla, Phytochemical constituents, Secondary metabolites, Patagonian species.

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Calafate, Berberis microphylla, Phytochemical constituents, Secondary metabolites, Patagonian species.





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Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 6 June 2017 Page: 225-227

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Authors & Affiliations

María Cristina Furrianca
Department of Nursing, Universidad de Magallanes, Chile.

Marysol Alvear
Department of Chemical Sciences and Natural Resources, Universidad de La Frontera, Temuco, Chile.

Tomás Zambrano
Department of Basic Sciences, Center of Molecular Biology and Pharmacogenetics, Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Temuco, Chile

Víctor Fajardo
Department of Science and Natural Resources, Universidad de Magallanes, Punta Arenas, Chile.

Luis Salazar
Department of Basic Sciences, Center of Molecular Biology and Pharmacogenetics, Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Temuco, Chile

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