PHYTOCHEMICAL CONSTITUENTS OF THE ROOT OF BERBERIS MICROPHYLLA

Authors

  • MARÍA CRISTINA FURRIANCA Department of Nursing, Universidad de Magallanes, Chile.
  • Marysol Alvear Department of Chemical Sciences and Natural Resources, Universidad de La Frontera, Temuco, Chile.
  • TOMÁS ZAMBRANO Department of Basic Sciences, Center of Molecular Biology and Pharmacogenetics, Scientific and TechnologicalBioresource Nucleus, Universidad de La Frontera, Temuco, Chile
  • VÍCTOR FAJARDO Department of Science and Natural Resources, Universidad deMagallanes, Punta Arenas, Chile.
  • Luis a Salazar Department of Basic Sciences, Center of Molecular Biology and Pharmacogenetics, Scientific and TechnologicalBioresource Nucleus, Universidad de La Frontera, Temuco, Chile

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i6.17803

Keywords:

Calafate, Berberis microphylla, Phytochemical constituents, Secondary metabolites, Patagonian species

Abstract

Objective: To objective of this work was to perform phytochemical qualitative and quantitative analyzes of the main secondary metabolites in the root of Berberis microphylla.

Methods: The extracts of B. microphylla root were tested through phytochemical screening and the quantification of the most important constituents was carried out using spectrophotometric and gravimetric techniques.

Results: Phytochemical screening of both extracts showed the presence of alkaloids, flavonoids, glycosides, cardiac glycosides, saponins, terpenes,

and tannins, which are pharmacologically important. Quantification of the major phytochemicals groups showed that the ethanolic extract contains 3.9% alkaloids, 0.46% flavonoids, 9.53% tannins, and 3.60% saponins. Similarly, the methanolic extract contains 6.61% alkaloids, 0.41% flavonoids, 7.40% tannins, and 1.43% saponins.

Conclusion: This is the first time that the presence of tannins, flavonoids, and saponins in this plant has been reported. The medicinal properties of the root of B. microphylla may exist due to the presence secondary metabolites.

Downloads

Download data is not yet available.

Author Biographies

MARÍA CRISTINA FURRIANCA, Department of Nursing, Universidad de Magallanes, Chile.

Nursing Department

Marysol Alvear, Department of Chemical Sciences and Natural Resources, Universidad de La Frontera, Temuco, Chile.

Center of Amelioration and Sustainability of Volcanic Soils, Scientific and Technological Bioresource Nucleus (BIOREN).

TOMÁS ZAMBRANO, Department of Basic Sciences, Center of Molecular Biology and Pharmacogenetics, Scientific and TechnologicalBioresource Nucleus, Universidad de La Frontera, Temuco, Chile

Center of Molecular Biology and Pharmacogenetics, Department of Basic Sciences; Scientific and Technological Bioresource Nucleus (BIOREN)

VÍCTOR FAJARDO, Department of Science and Natural Resources, Universidad deMagallanes, Punta Arenas, Chile.

Science Departament

Luis a Salazar, Department of Basic Sciences, Center of Molecular Biology and Pharmacogenetics, Scientific and TechnologicalBioresource Nucleus, Universidad de La Frontera, Temuco, Chile

Center of Molecular Biology and Pharmacogenetics, Department of Basic Sciences; Scientific and Technological Bioresource Nucleus (BIOREN)

References

Bottini MC, De Bustos A, Sanso AM, Jouve N, Poggio L. Relationships in Patagonian species of Berberis (Berberidaceae) based on the characterization of rDNA internal transcribed spacer sequences. Bot J Linn Soc 2007;153(3):321-8.

Ahrendt LW. Berberis and Mahonia. J Linn Soc London Bot 1961;57(369):1-410.

Landrum LR. Revision of Berberis (Berberidaceae) in Chile and adjacent Southern Argentina. Ann Missouri Bot Gard 1999;86(4):793-834. Available from: http://www.biostor.org/reference/13047. 4. Varas B, Castro MH, Rodriguez R, von Baer D, Mardones C, Hinrichsen P. Identification and characterization of microsatellites from calafate (Berberis microphylla, Berberidaceae). Appl Plant Sci 2013;1. pii: apps.1200003.

Sodagar N, Bahrami AR, Memariani F, Ejtehadi H, Vaezi J, Khosravi AR. Biosystematic study of the genus Berberis L. (Berberidaceae) in Khorassan, NE Iran. Plant Syst Evol 2012;298(1):193-203.

Mazumder PM, Das S, Das S, Das MK. Phyto-pharmacology of Berberis aristata DC: A Review. J Drug Deliv Ther 2011;1(2). Available from: http://www.jddtonline.info/index.php/jddt/article/view/34/25.

Singh V, Gunjan, Katiyar D. Anti-inflammatory activity of alcoholic and aqueous heartwood extracts of Berberis aristata DC. Asian J Pharm Clin Res 2014;7 Suppl 1:210-2.

Mokhber-Dezfuli N, Saeidnia S, Gohari AR, Kurepaz- Mahmoodabadi M. Phytochemistry and pharmacology of Berberis Species. Pharmacogn Rev 2014;8(15):8-15.

Ahamad J, Mir S, Naquvi K. Hypoglycemic activity of aqueous extract of Berberis aristata stems bark in stz-induced rats. Int J Pharm Pharm Sci 2012;4(2):473-4.

Upwar N, Patel R, Waseem N, Kumar Mahobia N. Hypoglycemic effect of methanolic extract of Berberis aristata DC stem on normal and streptozotocin induced diabetic rats. Int J Pharm Pharm Sci 2011;3(1):222-4.

Unkeshwar P, Nasiruddin M, Fayazuddin M, Khan R, Khan A, AQ3 Tajuddin. Evaluation of hepatoprotective activity of Berberis aristata against carbon tetrachloride induced hepatotoxicity in rats. Int J Pharm Pharm Sci 2013;5 Suppl 4:107-10.

Dehar N, Walia R, Verma RB, Pandey P. Hepatoprotective activity of Berberis aristata root extract against chemical induced acute hepatotoxicity in rats. Asian J Pharm Clin Res 2013;6 Suppl 5:53-6.

Alamzeb M, Khan MR, Ali S, Shah SQ, Rashid MU. Antimicrobial properties of extracts and compounds isolated from Berberis jaeschkeana. Bangladesh J Pharmacol 2013;8(2):107-9.

Mittal M, Juyal V, Singh A. Phytochemical, antidiabetic, and cytoprotective properties of Berberis aristata DC. root extracts. Pharm Crop 2012;3:64-8.

Patni S, Sah AN, Meena H, Pandey HK. Physico-chemical, phyto-chemical and elemental analysis of stem bark and roots of Berberis asiatica. Adv Appl Sci Res 2012;3(6):3624-8.

Arena ME, Curvetto N. Berberis buxifolia fruiting: Kinetic growth behavior and evolution of chemical properties during the fruiting period and different growing seasons. Sci Hortic (Amsterdam) 2008;118(2):120-7. Available from: http://www.linkinghub.elsevier. com/retrieve/pii/S0304423808002069. [Last cited on 2014 Sep 5].

Fajardo V, Urzúa A, Torres R, Cassels B. Secondary metabolites from Berberis buxifolia. Rev Latinoamer Quím 1979;10:131-4.

AQ4 Domínguez E. Flora de interés etnobotánico usada por los pueblos originarios: Aónikenk, Selk’nam, Kawésqar, Yagan y Haush en la Patagonia Austral. Dominguezia 2010;26(2):19-29.

Echeverria J, Niemeyer HM. Alkaloids from the native flora of Chile: A review. Bol Latinoam Caribe Plant Med Aromat 2012;11(4):291-305.

Manosalva L, Mutis A, Diaz J, Urzua A, Fajardo V, Quiroz A. Identification of isoquinoline alkaloids from Berberis microphylla by HPLC ESI-MS/MS. Bol Latinoam Caribe Plant Med Aromat 2014;13:323-34.

Gowri SS, Vasantha K. Phytochemical screening and antibacterial activity of Syzygium cumini (L.) (Myrtaceae) leaves extracts. Int J Pharm Tech Res 2010;2(2):1569-73.

Patel RK, Patel JB, Trivedi PD. Spectrophotometric method for the estimation of total alkaloids in the Tinospora cordifolia M. and its herbal formulations. Int J Pharm Pharm Sci 2015 20;7(10):249-51. Available from: http://www.innovareacademics.in/journals/index.php/ ijpps/article/view/7622/6129.

Shamsa F, Monsef H, Ghamooshi R, Verdian-rizi M. Spectrophotometric determination of total alkaloids in some Iranian medicinal plants. Thai J Pharm Sci 2008;32:17-20.

Naskar S, Mazumder UK, Pramanik G, Bala A, Haldar PK, Islam A, et al. Comparative in vitro antioxidant activity of different parts of Cocos nucifera (Linn.) on reactive oxygen and nitrogen species. Int J Pharm Pharm Sci 2011;3 Suppl 3:104-7.

Gulfraz M, Asad M, Qaddir G, Mehmood S, Shaukat S, Parveen Z. Phytochemical constituents of Berberis lycium royle and Justicia adhatoda. J Chem Soc Pak 2008;30:453-7.

Attarde D, Patil M, Chaudhari B, Pal S. Estimation of tannin content in some marketed Harde churna (Terminalia chebula Retz. Family- Combretaceae). Int J Pharm Technol 2010;2(3):750-6.

Doss A. Preliminary phytochemical screening of some Indian medicinal plants. Anc Sci Life 2009;29(2):12-6.

Gulfraz M, Mehmood S, Ahmad A, Fatima N, Praveen Z, Williamson EM. Comparison of the antidiabetic activity of Berberis lyceum root extract and berberine in alloxan-induced diabetic rats. Phyther Res 2008;22(9):1208-12.

Otshudi AL, Vercruysse A, Foriers A. Contribution to the ethnobotanical, phytochemical and pharmacological studies of traditionally used medicinal plants in the treatment of dysentery and diarrhoea in Lomela area, Democratic Republic of Congo (DRC). J Ethnopharmacol 2000;71(3):411-23. Available from: http://www.sciencedirect.com/ science/article/pii/S0378874100001677.

Tiwari AK, Rao JM. Diabetes mellitus and multiple therapeutic approaches of phytochemicals: Present status and future prospects. Curr Sci 2002;83(1):30-8.

Villinski J, Dumas E, Chai HB, Pezzuto J, Angerhofer C, Gafner S. Antibacterial activity and alkaloid content of Berberis thunbergii, Berberis vulgaris and Hydrastis canadensis. Pharm Biol 2003;41(8):551-7.

Andola HC, Gaira KS, Rawal RS, Rawat MS, Bhatt ID. Habitat-dependent variations in berberine content of Berberis asiatica Roxb. ex. DC. in Kumaon, Western Himalaya. Chem Biodivers 2010;7(2):415-20.

Maithani A, Parcha V, Kumar D. Quantitative estimation of berberine content of Berberis asiatica from different altitude of Garhwal Himalaya. Asian J Pharm Clin Res 2014;7 Suppl 1:165-7.

Patel DK, Patel K, Dhanabal SP. Standardization of Berberis aristata extract through conventional and modern HPTLC techniques. Asian Pac J Trop Dis 2012;2:S136-40.

Srivastava S, Rawat AK. Quality evaluation of ayurvedic crude drug daruharidra, its allied species, and commercial samples from herbal drug markets of India. Evid Based Complement Alternat Med 2013;2013:472973.

El Sayed M, Ghareeb DA, Khalil AA, Sarhan EM. Therapeutic bio-screening of the bioactive ingredients of Berberis vulgaris. Funct Plant Sci Biotechnol 2011;13:63-8.

Published

01-06-2017

How to Cite

MARÍA CRISTINA FURRIANCA, M. Alvear, TOMÁS ZAMBRANO, VÍCTOR FAJARDO, and L. a Salazar. “PHYTOCHEMICAL CONSTITUENTS OF THE ROOT OF BERBERIS MICROPHYLLA”. Asian Journal of Pharmaceutical and Clinical Research, vol. 10, no. 6, June 2017, pp. 225-7, doi:10.22159/ajpcr.2017.v10i6.17803.

Issue

Section

Original Article(s)