ANTIOXIDATIVE AND ANTIBACTERIAL POTENTIALS OF FUNGAL ENDOPHYTES FROM JUSTICIA WYNAADENSIS HEYNE: AN ETHNOMEDICINAL RAIN FOREST SPECIES OF WESTERN GHATS


Madhuchhanda Das, Harischandra Sripathy Prakash, Monnanda Somaiah Nalini

Abstract


Objective: Endophytes, living within the medicinal plants, are recognized as an alternative source of bioactive components useful for human health. This study aims to isolate and identify the fungal endophytes from the ethnomedicinal rain forest plant Justicia wynaadensis and evaluation of their antioxidant and antimicrobial potentials.

Methods: Endophytic fungi were isolated and identified by sequencing the internal transcribed spacer. The secondary metabolites were extracted with ethyl acetate and evaluated for the total phenolic, flavonoid, antioxidant, and antibacterial potentials. The antioxidative capacities were determined using different experimental models, viz., radical scavenging capacity, reducing power, and inhibition of lipid peroxidation. The antibacterial potential of extracts was determined through disc diffusion method and by evaluating minimum inhibitory concentration through microplate technique.

Results: A total of nine fungal endophytes belonging to six genera were identified. The total phenolic content of the extracts ranged from 4.8±0.08 to 20.74±0.96 mg Gallic acid equivalent/g dry extract. Flavonoid was present in eight extracts in the range of 2.1±0.08 to 8.75±0.6 mg catechin equivalent/g dry extract. Fusarium incarnatum was found to have potentially high antioxidant capacity as well as broad spectrum antibacterial activity against Gram-positive and Gram-negative bacteria.

Conclusion: This study reported various endophytic fungi from the plant J. wynaadensis. This is the first attempt to explore the treasure of endophytes and their bioactive potentiality from this plant. Hence, our findings encourage the exploration of these fungi and exploit them in search of potential drug discovery.


Keywords


Endophytic fungi, Justicia wynaadensis, Western Ghats, Antioxidant, Antibacterial, Fusarium incarnatum.

| PDF |

References


Balick MJ, Elisabetsky E, Laind SA. editors. Medicinal Resources of the Tropical Forest: Biodiversity and its Importance for Human Health. NewYork: Columbia University Press; 1996.

Strobel G, Daisy B. Bioprospecting for microbial endophytes and their natural products. Microbiol Mol Biol Rev 2003;67(4):491-502.

Bacon CW, White JF. Microbial Endophytes. NewYork: Marcel Dekker; 2000. p. 341-88.

Owen NL, Hundley N. Endophytes – The chemical synthesizers inside plants. Sci Prog 2004;87:79-99.

Strobel GA. Endophytes as sources of bioactive products. Microbes Infect 2003;5(6):535-44.

Strobel G, Daisy B, Castillo U, Harper J. Natural products from endophytic microorganisms. J Nat Prod 2004;67(2):257-68.

Tan RX, Zou WX. Endophytes: A rich source of functional metabolites. Nat Prod Rep 2001;18(4):448-59.

Gunatilaka AA. Natural products from plant-associated microorganisms: Distribution, structural diversity, bioactivity, and implications of their occurrence. J Nat Prod 2006;69(3):509-26.

Huang WY, Cai YZ, Xing J, Corke H, Sun M. A potential antioxidant resource: Endophytic fungi from medicinal plants. Econ Bot 2007;61:14-30.

Karmakar R, Sunil K, Prakash HS. Antioxidant, anti-inflammatory, antimicrobial and cytotoxic properties of fungal endophytes from Garcinia species. Int J Pharm Pharm Sci 2013;5(3):889-97.

Das M, Nalini MS, Prakash HS. Antioxidative properties of phenolic compounds isolated from the fungal endophytes of Zingiber nimmonii (J. Graham) Dalzell. Front Biol 2017. DOI: 10.1007/s11515-016-1441-z.

Gamble JS. Flora of the Presidency of Madras. Vol. II. London: Adlard and Sons Ltd.; 1967. p. 755-6.

Awan AJ, Ahmed CB, Uzair M, Aslam MS, Farooq U, Ishfaq K. Family acanthaceae and genus Aphelandra: Ethnopharmacological and phytochemical review. Int J Pharm Pharm Sci 2014;6(10):44-55.

Lingaraju, DP, Sudarshana MS, Rajashekar N. Ethnopharmacological survey of traditional medicinal plants in tribal areas of Kodagu district, Karnataka, India. J Pharm Res 2013;6:284-97.

Subbiah MT, Norman EJ. Rain forest plant extract with cellular cholesterol lowering properties. U S Pat 2002;6:365-411.

Tejesvi MV, Mahesh B, Nalini MS, Prakash HS, Kini KR, Subbiah V, et al. Endophytic fungal assemblages from the inner bark and twig of Terminalia arjuna W. and A. (Combretaceae). World J Microbiol Biotechnol 2005;21:1535-40.

Schulz B, Guske S, Dammann U, Boyle C. Endophyte host interactions II. Defining symbiosis of the endophyte host interaction. Symbiosis 1998;25:213-27.

Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, et al. Current protocols in molecular biology. New York: Wiley; 1994.

Liu X, Dong M, Chen X, Jiang M, Lv X, Yan G. Antioxidant activity and phenolics of an endophytic Xylaria sp. from Ginkgo biloba. Food Chem 2007;105:548-54.

Barros L, Ferreira MJ, Queirós B, Ferreira CF, Baptista P. Total phenols, ascorbic acid, β-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities. Food Chem 2007;103:413-9.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Evans CR. Antioxidant activity applying and improved ABTS radical cation decolorization assay. Free Radic Biol Med 1999;26:1231-7.

Oyaizu M. Studies on product of browning reaction prepared from glucose amine. J Nutr 1986;44:307-15.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95(2):351-8.

Bauer AW, Kirby WM, Sherries JC, Turck M. Antibiotics susceptibility testing by the standardized single disc method. Am J ClinPathol 1966;45:493-6.

Xu L, Zhou L, Li J, Li X, Wang J. Fungal endophytes from Dioscorea zingiberensis and their antimicrobial activity. Lett Appl Microbiol 2008;46:68-72.

Nalini MS, Sunayana N, Prakash HS. Endophytic fungal diversity in medicinal plants of Western Ghats, India. Int J Biodiv 2014. DOI: 10.1155/2014/494213.

Akshatha VJ, Nalini MS, D’Souza C, Prakash HS. Streptomycete endophytes from anti-diabetic medicinal plants of the Western Ghats inhibit alpha-amylase and promote glucose uptake. Lett Appl Microbiol 2014;58:433-9.

Samaga PV, Rai VR. Diversity and bioactive potential of endophytic fungi from Nothapodytes foetida, Hypericum mysorense and Hypericum japonicum collected from Western Ghats of India. Ann Microbiol 2016;66:229-44.

Gangadevi V, Muthumary J. Isolation of Colletotrichum gloeosporioides, a novel endophytic taxol-producing fungus from the leaves of a medicinal plant, Justicia gendarussa. Mycol Balc 2008;5:1-4.

Medapa S, Singh GR, Ravikumar V. The phytochemical and antioxidant screening of Justicia wynaadensis. Afr J Plant Sci 2011;5(9):489-92.

Abhishek M, Jain K, Somashekaraiah BV, Raman DN. Phytochemical composition and in-vitro antioxidant activity of Justicia wynaadensis leaves. IJIRSET 2015;4(9):8236-42.

Tuma DJ, Casey CA. Dangerous byproducts of alcohol breakdown—focus on adducts. Alcohol Res Health 2003;27:285-90.

Onyema OO, Farombi EO, Emerole GO, Ukoha AI, Onyeze GO. Effect of vitamin E on monosodium glutamate induced hepatotoxicity and oxidative stress in rats. Indian J Biochem Biophys 2006;43(1):20-4.

Srivastava A, Raveesha KA. Antimicrobial potential of fungal endophytes isolated from leaves of Prosopis juliflora (sw.) Dc. an important weed. Int J Pharm Pharm Sci 2015;7(12):128-36.

Sahu R, Kumar S, Aharwal RP, Sandhu SS. Antibacterial activity of isolated endophytic fungi from Rauvolfia serpentina (L.) Benth. Ex kurz. Int J Pharm Pharm Sci 2016;8(11):38-42.

Prabavathy D, Nachiyar CV. Study on the antimicrobial activity of Aspergillus sp isolated from Justicia adathoda. Indian J Sci Technol 2012;5(9):3317-20.




About this article

Title

ANTIOXIDATIVE AND ANTIBACTERIAL POTENTIALS OF FUNGAL ENDOPHYTES FROM JUSTICIA WYNAADENSIS HEYNE: AN ETHNOMEDICINAL RAIN FOREST SPECIES OF WESTERN GHATS

Keywords

Endophytic fungi, Justicia wynaadensis, Western Ghats, Antioxidant, Antibacterial, Fusarium incarnatum.

DOI

10.22159/ajpcr.2017.v10i6.18106

Date

01-06-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 6 June 2017 Page: 203-209

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

81 Views | 90 Downloads

Authors & Affiliations

Madhuchhanda Das
Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore - 570 006, Karnataka, India.
India

Harischandra Sripathy Prakash
Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore - 570 006, Karnataka, India.
India

Monnanda Somaiah Nalini
Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore - 570 006, Karnataka, India.
India


Article Tools


Email this article (Login required)
Email the author (Login required)

Refbacks

  • There are currently no refbacks.