• Yuvarani Thambidurai Department of Bioinformatics, Bharathiar University, Coimbatore, Tamil Nadu, India.
  • Sudarsanam D Department of Biotechnology, D.G. Vaishnav College, Arumbakkam, Chennai - 600106, Tamil Nadu, India.
  • Habeeb Skm Department of Genetic Engineering, School of Bioengineering, SRM University, Chennai - 603 203, Tamil Nadu, India.
  • Ashok Kumar T Naturophathy and Yoga Therapist, Zero Error, Dharmapuri - 636 705, Tamil Nadu, India.



2, Nil, 2-diphenyl-1-picrylhydrazyl, Ferric reducing antioxidant power, Antioxidant activity, Marine sponges


Objective: The main focus of this study is to screen the marine sponges for potent free radical scavenging activity.

Methods: Various methods such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) assay are employed to ascertain the antioxidant properties of marine sponges namely Dysidea herbacea and Sigmadocia pumila.

Results: On analyzing, the result of ABTS assay D. herbacea and S. pumila exhibited almost equal antioxidant properties. While calculating the inhibitory concentration 50% value for DPPH assay, the Sample 1 and 2 has an IC of 655.49 and 826.739 μl, respectively, and in FRAP assay, the Sample 1 and 2 has an IC of 67.587 and 74.57 μg, respectively.

Conclusion: Overall from this assay, D. herbacea revealed slightly better antioxidant activity when compared to S. pumila, also which in future may serve as a better source to fight against various diseases


Download data is not yet available.

Author Biography

Yuvarani Thambidurai, Department of Bioinformatics, Bharathiar University, Coimbatore, Tamil Nadu, India.

Assistant Professor,

Department of Biotechnology, 

D.G. Vaishnav College


Higa T, Tanaka JI, Kitamura A, Koyama T, Takahashia M, Uchida T. Bioactive compounds from marine sponges. Pure Appl Chem 1994;66:2227-30.

Haefner B. Drugs from the deep: Marine natural products as drug candidates. Drug Discov Today 2003;8(12):536-44.

Belarbi H, Contreras Gómez A, Chisti Y, García Camacho F, Molina Grima E. Producing drugs from marine sponges. Biotechnol Adv 2003;21(7):585-98.

Sipkema D, Franssen MC, Osinga R, Tramper J, Wijffels RH. Marine sponges as pharmacy. Mar Biotechnol (NY) 2005;7(3):142-62.

Faulkner DJ. Marine natural products. Nat Prod Rep 2000;17(1):7-55.

Faulkner DJ. Marine natural products. Nat Prod Rep 2001;18(1):1-49.

Faulkner DJ. Marine natural products. Nat Prod Rep 2002;19(1):1-48.

Tramper J. Cultivation of marine sponges. Detmer Sipkema. The Netherlands: Process Engineering Group, Wageningen University; 1999. p. 184.

Aneiros A, Garateix A. Bioactive peptides from marine sources: Pharmacological properties and isolation procedures. J Chromatogr B Analyt Technol Biomed Life Sci 2004;803(1):41-53.

Müller WE, Schröder HC, Wiens M, Perovic-Ottstadt S, Batel R, Müller IM. Traditional and modern biomedical prospecting: Part II-the benefits: Approaches for a sustainable exploitation of biodiversity (secondary metabolites and biomaterials from sponges). Evid Based Complement Alternat Med 2004;1(2):133-44.

Thakur NL, Hentschel U, Krasko A, Anil AC, WE Müller. Antibacterial activity of the sponge suberites domuncula and its primmorphs: Potential basis for chemical defense. Aquat Microbial Ecol 2003;31:77-83.

Mandal S, Yadav S, Yadav S, Nema RK. Antioxidants: A review. J Chem Pharm Res 2009;1(1):102-4.

Tiwari AK. Antioxidants: New - Generation therapeutic base for treatment of polygenic disorders. Curr Sci 2004;86:1092-102.

Shinde A, Ganu J, Naik P. Effect of free radicals and antioxidants on oxidative stress. J Dent Allied Sci 2012;1:63-6.

Chance B, Sies H, Boveris A. Hydroperoxide metabolism in mammalian organs. Physiol Rev 1979;59(3):527-605.

de Groot H. Reactive oxygen species in tissue injury. Hepatogastroenterology 1994;41(4):328-32.

Nakazawa H, Genka C, Fujishima M. Pathological aspects of active oxygens/free radicals. Jpn J Physiol 1996;46(1):15-32.

Toyokuni S. Reactive oxygen species-induced molecular damage and its application in pathology. Pathol Int 1999;49(2):91-102.

Warier VP. Indian Medicinal Plants: A Compendium of 500 Species, I. Kottakkal-India: Orient Longman Publishing House; 2002. p. 146.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 1999;26:1231-7.

Benzie FF, Strain JJ. Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol 1999;299:15-23.

Ahmed M, Saeed F, Mehjabeen, Jahan N. Evaluation of insecticidal and antioxidant activity of selected medicinal plants. J Pharm Phytochem 2013;2(3):153-8.

Patel RM, Patel NJ. In vitro antioxidant activity of coumarin compounds by DPPH, superoxide and nitric oxide free radical scavenging methods. J Adv Pharm Educ Res 2011;1:52-68.

Koleva II, van Beek TA, Linssen JP, de Groot A, Evstatieva LN. Screening of plant extracts for antioxidant activity: A comparative study on three testing methods. Phytochem Anal 2002;13(1):8-17.

Bhakuni DS, Rawat DS. Bioactive Marine Natural Products. New Delhi,India: Springer and Anamaya Publishers; 2005.

Newbold RW, Jensen PR, Fenical W, Pawlik JR. Antimicrobial activity of caribbean sponge extracts. Aquat Microbial Ecol 1999;19:279-84.

Faulkner DJ. Highlights of marine natural products chemistry (1972- 1999). Nat Prod Rep 2000;17(1):1-6.

Rangel M, Sanctis B, Freitas JC, Polatto JM, Granato AC, Berlinck RG, et al. Cytotoxic and neurotoxic activities in extracts of marine sponges from southeastern Brazilian coast. J Exp Biol Ecol 2001;262(1):31-40.

Touati I, Chaieb K, Bakhrouf A, Gaddour K. Screening of antimicrobial activity of marine sponge extracts collected from Tunisian coast. J Med Mycol 2007;17(3):183-6.

Ferreira M, Cabado AG, Chapela MJ, Fajardo P, Atanassova M, Garrido A, et al. Cytotoxic activity of extracts of marine sponges from NW Spain on a neuroblastoma cell line. Environ Toxicol Pharmacol 2011;32(3):430-7.

Lakshmi V, Mishra SK, Srivastava S, Chaturvedi A, Srivastava MN, Shukla PK. Antifungal activity of marine sponge haliclonaexigua (krikpatrick). J Med Mycol 2010;20:31-3.

Li H, Shigeki S, Fusetani N. Simple antifungal metabolites from a marine sponge, Halichondria sp. Comp Biochem Physiol B Comp Biochem 1994;107(2):261-4.

Chairman K, Ranjit Singh AJ, Alagumuthu G. Cytotoxic and antioxidant activity of selected marine sponges. Asian Pac J Trop Dis 2012;2:234-8.

Wickens AP. Ageing and the free radical theory. Respir Physiol 2001;128(1):379-91.

Ozyürek M, Bektasoglu B, Güçlü K, Apak R. Hydroxyl radical scavenging assay of phenolics and flavonoids with a modified cupric reducing antioxidant capacity (CUPRAC) method using catalase for hydrogen peroxide degradation. Anal Chim Acta 2008;616(2):196-206.

Halliwell B. Free radicals, antioxidants, and human disease: Curiosity, cause, or consequence? Lancet 1994;344(8924):721-4.

Rajani P, Kotaiah MR, Jayaveera KN, Sekhar KB. Evaluation of antioxidant and anticancer activities of cipadessa baccifera. Asian J Pharm Clin Res 2015;8(5):312-5.

Rani SA, Sundaram L, Vasantha PB. In vitro antioxidant and anticancer activity of l-asparaginase from Aspergillus flavus (kufs20). Asian J Pharm Clin Res 2011;4 Suppl 2:174-7.

Arsianti AA, Fadilah F, Yazid F, Wibisono LK, Suid K, Azizah NN, et al. Phytochemical composition and anticancer activity of seaweeds ulva lactuca and Eucheuma cottonii against breast Mcf-7 and colon Hct-116 cells. Asian J Pharm Clin Res 2016;9(6):115-9.

Vichit W, Saewan N. Antioxidant activities and cytotoxicity of thai pigmented rice. Int J Pharm Pharm Sci 2015;7(7):329-4.

Fitria A, Andriania M, Sudarmanb A, Toharmatb T, Yonekurac L, Tamurac H, et al. Screening of antioxidant activities and their bioavailability of tropical fruit byproducts from Indonesia. Int J Pharm Pharm Sci 2016;8(6):96-100.



How to Cite

Thambidurai, Y., S. D, H. Skm, and A. K. T. “FREE RADICAL SCAVENGING ACTIVITY OF MARINE SPONGES COLLECTED FROM KOVALAM, CHENNAI”. Asian Journal of Pharmaceutical and Clinical Research, vol. 10, no. 6, June 2017, pp. 321-7, doi:10.22159/ajpcr.2017.v10i6.18115.



Original Article(s)