ANTINUCLEAR ANTIBODIES IN PATIENTS WITH UNEXPLAINED RECURRENT ABORTIONS


Anupriya Asaithambi, Manjula Gunasekaran, Manivelan S, Palaniappan Nainar

Abstract


Objective:This study aims to determine the prevalence of antinuclear antibodies in pregnant women with bad obstetric history (BOH) against healthy multigravid women in and around Tirunelveli district

Methods: This is a case-control study comprising 60 antenatal women with BOH against 60 multiparous pregnant women with no history of abortions. Antinuclear antibodies (ANA) were detected using indirect immunofluorescence with Hep-2 cell substrate, and enzyme-linked immunosorbent assay (ELISA).

Results: Among BOH cases 19 (82.6%), 18 (78.26%) were positive by ELISA and indirect fluorescence antibody test (IFAT) method, respectively. Among controls, 4(17.39%) and 5(21.73%) individuals were positive by ELISA and IFAT methods, respectively. Of the 18 positives, homogenous pattern was most common followed by anticentromere pattern, fine speckled and coarse speckled pattern

Conclusion: IFAT is considered to be gold standard in the diagnosis of autoimmune disorders, but ELISA appears to be a suitable simple alternative for testing rheumatological disorders.


Keywords


Antinuclear antibodies, Bad obstetric history, Indirect immunofluorescence.

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References


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About this article

Title

ANTINUCLEAR ANTIBODIES IN PATIENTS WITH UNEXPLAINED RECURRENT ABORTIONS

Keywords

Antinuclear antibodies, Bad obstetric history, Indirect immunofluorescence.

DOI

10.22159/ajpcr.2017.v10i8.18343

Date

01-08-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 8 August 2017 Page: 256-259

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

85 Views | 171 Downloads

Authors & Affiliations

Anupriya Asaithambi
Department of Microbiology, Chennai Medical College Hospital and Research Centre, Irungalur, Trichy - 620 105, Tamil Nadu, India.
India

Manjula Gunasekaran
Department of Microbiology, Madurai Medical College, Madurai, Tamil Nadu, India
India

Manivelan S
Department of Microbiology, Chennai Medical College Hospital and Research Centre, Irungalur, Trichy - 620 105, Tamil Nadu, India.
India

Palaniappan Nainar
Department of Microbiology, Sree Mookambika Institute of Medical Sciences, Kulasekharam, Tamil Nadu, India
India


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