ANTINUCLEAR ANTIBODIES IN PATIENTS WITH UNEXPLAINED RECURRENT ABORTIONS

  • Anupriya Asaithambi Department of Microbiology, Chennai Medical College Hospital and Research Centre, Irungalur, Trichy - 620 105, Tamil Nadu, India.
  • Manjula Gunasekaran Department of Microbiology, Madurai Medical College, Madurai, Tamil Nadu, India
  • Manivelan S Department of Microbiology, Chennai Medical College Hospital and Research Centre, Irungalur, Trichy - 620 105, Tamil Nadu, India.
  • Palaniappan Nainar Department of Microbiology, Sree Mookambika Institute of Medical Sciences, Kulasekharam, Tamil Nadu, India

Abstract

Objective:This study aims to determine the prevalence of antinuclear antibodies in pregnant women with bad obstetric history (BOH) against healthy multigravid women in and around Tirunelveli district

Methods: This is a case-control study comprising 60 antenatal women with BOH against 60 multiparous pregnant women with no history of abortions. Antinuclear antibodies (ANA) were detected using indirect immunofluorescence with Hep-2 cell substrate, and enzyme-linked immunosorbent assay (ELISA).

Results: Among BOH cases 19 (82.6%), 18 (78.26%) were positive by ELISA and indirect fluorescence antibody test (IFAT) method, respectively. Among controls, 4(17.39%) and 5(21.73%) individuals were positive by ELISA and IFAT methods, respectively. Of the 18 positives, homogenous pattern was most common followed by anticentromere pattern, fine speckled and coarse speckled pattern

Conclusion: IFAT is considered to be gold standard in the diagnosis of autoimmune disorders, but ELISA appears to be a suitable simple alternative for testing rheumatological disorders.

Keywords: Antinuclear antibodies, Bad obstetric history, Indirect immunofluorescence.

Author Biographies

Anupriya Asaithambi, Department of Microbiology, Chennai Medical College Hospital and Research Centre, Irungalur, Trichy - 620 105, Tamil Nadu, India.

Asst Professor in Microbiology,

Chennai Medical College Hospital & research Centre

Manjula Gunasekaran, Department of Microbiology, Madurai Medical College, Madurai, Tamil Nadu, India

Asst Prof in Microbiology,

Madurai Medical College

References

1. Shankarakumar U, Pradhan VD, Patwardhan MM, Shankarakumar A, Ghosh K. Autoantibody profile and other immunological parameters in recurrent spontaneous abortion patients. Niger Med J 2011;52:3.
2. Pandey MK, Rani R, Agrawal S. An update in recurrent spontaneous abortion. Arch Gynecol Obstet 2005;272(2):95-108.
3. Internet Reference. Available from: http://www.srm-ejournal.com/article.asp?AID=10215&UIB. [Last accessed on 2012 Nov].
4. Kumar Y, Bhatia A, Minz RW. Antinuclear antibodies and their detection methods in diagnosis of connective tissue diseases. Diagn Pathol 2009;4:1.
5. Patton PE, Coulam CB, Bergstralh E. The prevalence of autoantibodies in pregnant and nonpregnant women. Am J Obstet Gynecol 1987;157(6):1345-50.
6. Garcia-De La Torre I, Hernandez-Vazquez L, Angulo-Vazquez J, Romero-Ornelas A. Prevalence of antinuclear antibodies in patients with habitual abortion and in normal and toxemic pregnancies. Rheumatol Int 1984;4(2):87-9.
7. Nakatsuka M, Yoshida N, Hasegawa A, Nishikori K, Hirano Y, Katayama T, et al. Antinuclear antibody measurement as a screening test for sterile and infertile women with immunological abnormality. Nihon Sanka Fujinka Gakkai Zasshi 1993;45(5):431-6.
8. Farnam J, Lavastida MT, Grant JA, Reddi RC, Daniels JC. Antinuclear antibodies in the serum of normal pregnant women: A prospective study. J Allergy Clin Immunol 1984;73:596-9.
9. Xu L, Chang V, Murphy A, Rock JA, Damewood M, Schlaff W, et al. Antinuclear antibodies in sera of patients with recurrent pregnancy wastage. Am J Obstet Gynecol 1990;163:1493-7.
10. Satoh M, Chan EK, Ho LA, Rose KM, Parks CG, Cohn RD, et al. Prevalence and sociodemographic correlates of antinuclear antibodies in the United States. Arthritis Rheum 2012;64(7):2319-27.
11. Afman IE, Cronjé HS, Joubert G, Badenhorst PN, Schoon MG. Antinuclear antibody testing in obstetric patients. S Afr Med J 2003;93(12):932-7.
12. Bahar AM, Kwak JY, Beer AE, Kim JH, Nelson LA, Beaman KD, et al. Antibodies to phospholipids and nuclear antigens in non-pregnant women with unexplained spontaneous recurrent abortions. J Reprod Immunol 1993;24(3):213-22.
13. Kumagai S, Hayashi N. Immunofluorescence - Still the ‘gold standard’ in ANA testing? Scand J Clin Lab Invest Suppl 2001;235:77-83.
14. Divate S, Hardikar P, Bichile LS, Rajadhyaksha A. Clinical utility of screening for antinuclear antibodies by enzyme immunoassay - A preliminary study. J Assoc Physicians India 2004;52:290-3.
15. Tozzoli R, Bizzaro N, Tonutti E, Villalta D, Bassetti D, Manoni F, et al. Guidelines for the laboratory use of autoantibody tests in the diagnosis and monitoring of autoimmune rheumatic diseases. Am J Clin Pathol 2002;117(2):316-24.
Statistics
258 Views | 708 Downloads
Citatons
How to Cite
Asaithambi, A., M. Gunasekaran, M. S, and P. Nainar. “ANTINUCLEAR ANTIBODIES IN PATIENTS WITH UNEXPLAINED RECURRENT ABORTIONS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 8, Aug. 2017, pp. 256-9, doi:10.22159/ajpcr.2017.v10i8.18343.
Section
Original Article(s)