• Yuandani Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, 5 Jalan Almamater, USU-Kampus, Medan 20155, Indonesia.
  • Edy Suwarso Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, 5 Jalan Almamater, USU-Kampus, Medan 20155, Indonesia.



Curcuma mangga, Phagocytosis, Total leukocyte count, Immunomodulatory


Objective: This study was conducted to evaluate the immunomodulatory effects of ethanol extract of Curcuma mangga by in vivo study.

Methods: The ethanol extract of C. mangga was comprised to carbon clearance method for its immunomodulatory potential. The extract wasadministered orally at doses of 100, 200, and 400 mg/kg BW to mice for 7 days. On day 8, carbon ink was injected, and the blood was collected formeasurement of elimination of carbon. Total leukocyte count was also determined.

Results: The evaluation of immunomodulatory potential of ethanol extract of C. mangga revealed a dose-dependent increase in phagocytosis ability.The phagocytic index of ethanol extract of C. mangga was more than those of negative control, indicating the immunostimulatory activity of C. mangga.It showed low stimulation on total leukocyte count.Conclusion: The results indicate that ethanol extract of C. mangga rhizomes possesses immunomodulatory activity and has therapeutic potential forthe treatment of infectious diseases.


Download data is not yet available.



Elgert KD. Immunology Understanding the Immune System. 2nd ed. Virginia: A John Wiley & Sons, Inc.; 2009.

Kobayashi SD, Voyich JM, Burlak C, DeLeo FR. Neutrophils in the innate immune response. Arch Immunol Ther Exp (Warsz) 2005;53(6):505-17.

Bogdan C. Nitric oxide and the immune response. Nat Immunol 2001;2:907-16.

Leiro J, Alvarez E, Arranz JA, Laguna R, Uriarte E, Orallo F. Effects of cis-resveratrol on inflammatory murine macrophages: Antioxidant activity and down-regulation of inflammatory genes. J Leukoc Biol 2004;75(6):1156-65.

Diwanay S, Chitre D, Patwardhan B. Immunoprotection by botanical drugs in cancer chemotherapy. J Ethnopharmacol 2004;90(1):49-55.

Gautam M, Diwanay S, Gairola S, Shinde Y, Patki P, Patwardhan B. Immunoadjuvant potential of Asparagus racemosus aqueous extract in experimental system. J Ethnopharmacol 2004;91(2-3):251-5.

Jayathirtha MG, Mishra SH. Preliminary immunomodulatory activities of methanol extracts of Eclipta alba and Centella asiatica. Phytomedicine 2004;11(4):361-5.

Yu L, Zhao M, Yang B, Bai W. Immunomodulatory and anti-cancer activities of phenolics from Garcinia mangostana fruit pericarp. Food Chem 2009;116(4):969-73. Available from: http://www.sciencedirect. com/science/article/pii/S0308814609003628.

Yuandani, Jantan I, Ilangkovan M, Husain K, Chan KM. Inhibitory effects of compounds from Phyllanthus amarus on nitric oxide production, lymphocyte proliferation, and cytokine release from phagocytes. Drug Des Devel Ther 2016;10:1935-45.

Nassara M, Zerizera S, Kabouchec Z, Kabouchec A, Bechkric S. Antioxidant and the immunomodulatory activities exhibited by three plants from Lamiaceae family. Int J Pharm Pharm Sci 2015;7(9):331-4. Available from: ijpps/article/view/4618/2927.

Aichour R, Charef N, Baghiani A, Arrar L. Immunomodulatory effects of algerian caper. Int J Pharm Pharm Sci 2015;8(2):51-4. Available from: article/view/9779/3814.

Hong GW, Hong SL, Lee GS, Yaacob H, Malek SN. Non-aqueous extracts of Curcuma mangga rhizomes induced cell death in human colorectal adenocarcinoma cell line (HT29) via induction of apoptosis and cell cycle arrest at G0/G1 phase. Asian Pac J Trop Med 2016;9(1):8-18.

Malek SN, Lee GS, Hong SL, Yaacob H, Wahab NA, Faizal Weber JF, et al. Phytochemical and cytotoxic investigations of Curcuma mangga rhizomes. Molecules 2011;16(6):4539-48.

Abas F, Lajis NH, Shaari K, Israf DA, Stanslas J, Yusuf UK, et al. A labdane diterpene glucoside from the rhizomes of Curcuma mangga. J Nat Prod 2005;68(7):1090-3.

Ruangsang P, Tewtrakul S, Reanmongkol W. Evaluation of the analgesic and anti-inflammatory activities of Curcuma mangga Val and Zijp rhizomes. J Nat Med 2010;64(1):36-41.

Karsono AH, Tandrasasmita OM, Tjandrawinata RR. Molecular effects of bioactive fraction of Curcuma mangga (DLBS4847) as a downregulator of 5α-reductase activity pathways in prostatic epithelial cells. Cancer Manage Res 2014;6:267-78. Available from: http://www.

Abas F, Lajis NH, Israf DA, Khozirah S, Kalsom YU. Antioxidant and nitric oxide inhibition activities of selected Malay traditional vegetables. Food Chem 2006;95:566-73. Available from: http://www.

Jantan I, Yassin MA, Chin CB, Chen LL, Sim NL. Anti-fungal activity of the essential oils of nine Zingiberaceae species. Pharm Biol 2003;41(5):392-7. Available from: abs/10.1076/phbi.41.5.392.15941.

Yuandani, Suwarso E. Acute toxicity evaluation of ethanol extract of Curcuma mangga rhizome. Asian J Pharm Clin Res 2017;10(1):383-5. Available from: ajpcr/article/download/16196/9671.

Shukla S, Mehta A, John J, Mehta P, Vyas SP, Shukla S. Immunomodulatory activities of the ethanolic extract of Caesalpinia bonducella seeds. J Ethnopharmacol 2009;125(2):252-6.

Filias A, Theodorou GL, Mouzopoulou S, Varvarigou AA, Mantagos S, Karakantza M. Phagocytic ability of neutrophils and monocytes in neonates. BMC Pediatr 2011;11:29.

Burger RA, Torres AR, Warren RP, Caldwell VD, Hughes BG. Echinacea-induced cytokine production by human macrophages. Int J Immunopharmacol 1997;19(7):371-9.

Greenberg S, Grinstein S. Phagocytosis and innate immunity. Curr Opin Immunol 2002;14(1):136-45.

Harun NH, Septama AW, Jantan I. Immunomodulatory effects of selected Malaysian plants on the CD18/11a expression and phagocytosis activities of leukocytes. Asian Pac J Trop Biomed 2015;5(1):48-53. Available from: S2221169115301702.

Skubixz KM, Hammerschmidt DE. Effects of ibuprofen on chemotactic peptide-receptor binding and granulocyte response. Biochem Pharmacol 2011;35:3349-54. Available from: http://www.sciencedirect. com/science/article/pii/000629528690434X.

Jantan I, Harun NH, Septama AW, Murad S, Mesaik MA. Inhibition of chemiluminescence and chemotactic activity of phagocytes in vitro by the extracts of selected medicinal plants. J Nat Med 2011;65(2):400-5.



How to Cite

Yuandani, and E. Suwarso. “IMMUNOMODULATORY EFFECTS OF ETHANOL EXTRACT OF CURCUMA MANGGA RHIZOMES IN MICE”. Asian Journal of Pharmaceutical and Clinical Research, vol. 10, no. 9, Sept. 2017, pp. 148-50, doi:10.22159/ajpcr.2017.v10i9.18398.



Original Article(s)