SPECTROPHOTOMETRIC EVALUATION OF NIMESULIDE IMPURITY D: 4-NITRO-2- PHENOXYANILINE USING 8-HYDROXYQUINOLINE AS OXIDATIVE COUPLING REAGENT


Anil Kumar S, Bhaskar Bl

Abstract


Objective: Spectrophotometric investigation of 4-nitro-2-phenoxyaniline (4N2PA), a probable intermediate during the synthesis of anti-inflammatory drug nimesulide, both in pure form and in the presence of nimesulide has been attempted.

Methods: 4N2PA on diazotization followed by coupling with 8-hydroxyquinoline produces a crimson-colored complex which changes to deep violet on diluting with ethanol.

Results: The complex showed maximum absorbance at 560 nm when evaluated spectrophotometrically with a detection limit of 0.005 μg/ml and quantification range of 0.05-3.0 μg/ml. The method has been statistically evaluated with respect to the International Council for Harmonisation guidelines and found to be accurate and precise.

Conclusion: Pure tablet formulations of nimesulide do not respond to the method; however, the presence of minute amounts of 4N2PA in the drug as added impurity could be spectrophotometrically analyzed. Hence, the authors suggest that the reported technique could be a marker test for detecting the presence of 4N2PA in nimesulide formulations.


Keywords


4-Nitro2-phenoxyaniline, Nimesulide impurity, Spectrophotometry, 8-Hydroxyquinoline.

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References


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About this article

Title

SPECTROPHOTOMETRIC EVALUATION OF NIMESULIDE IMPURITY D: 4-NITRO-2- PHENOXYANILINE USING 8-HYDROXYQUINOLINE AS OXIDATIVE COUPLING REAGENT

Keywords

4-Nitro2-phenoxyaniline, Nimesulide impurity, Spectrophotometry, 8-Hydroxyquinoline.

DOI

10.22159/ajpcr.2017.v10i7.18540

Date

01-07-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 7 July 2017 Page: 204-206

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

187 Views | 150 Downloads

Authors & Affiliations

Anil Kumar S
Department of Chemistry, Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Amrita University, Bengaluru, Karnataka, India.
India

Bhaskar Bl
Department of Chemistry, Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Amrita University, Bengaluru, Karnataka, India.
India


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