SPECTROPHOTOMETRIC EVALUATION OF NIMESULIDE IMPURITY D: 4-NITRO-2- PHENOXYANILINE USING 8-HYDROXYQUINOLINE AS OXIDATIVE COUPLING REAGENT

Authors

  • Anil Kumar S Department of Chemistry, Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Amrita University, Bengaluru, Karnataka, India.
  • Bhaskar Bl Department of Chemistry, Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Amrita University, Bengaluru, Karnataka, India.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i7.18540

Keywords:

4-Nitro2-phenoxyaniline, Nimesulide impurity, Spectrophotometry, 8-Hydroxyquinoline

Abstract

Objective: Spectrophotometric investigation of 4-nitro-2-phenoxyaniline (4N2PA), a probable intermediate during the synthesis of anti-inflammatory drug nimesulide, both in pure form and in the presence of nimesulide has been attempted.

Methods: 4N2PA on diazotization followed by coupling with 8-hydroxyquinoline produces a crimson-colored complex which changes to deep violet on diluting with ethanol.

Results: The complex showed maximum absorbance at 560 nm when evaluated spectrophotometrically with a detection limit of 0.005 μg/ml and quantification range of 0.05-3.0 μg/ml. The method has been statistically evaluated with respect to the International Council for Harmonisation guidelines and found to be accurate and precise.

Conclusion: Pure tablet formulations of nimesulide do not respond to the method; however, the presence of minute amounts of 4N2PA in the drug as added impurity could be spectrophotometrically analyzed. Hence, the authors suggest that the reported technique could be a marker test for detecting the presence of 4N2PA in nimesulide formulations.

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References

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Published

01-07-2017

How to Cite

S, A. K., and B. Bl. “SPECTROPHOTOMETRIC EVALUATION OF NIMESULIDE IMPURITY D: 4-NITRO-2- PHENOXYANILINE USING 8-HYDROXYQUINOLINE AS OXIDATIVE COUPLING REAGENT”. Asian Journal of Pharmaceutical and Clinical Research, vol. 10, no. 7, July 2017, pp. 204-6, doi:10.22159/ajpcr.2017.v10i7.18540.

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