Sathya N Prasad, Murali Muralidhara


Objective: The aim of this study was to analyze antioxidant effect of geraniol (GE) in different in vitro models.

Methods: Initially, the antioxidant activity of GE was assessed by diphenyl picrylhydrazyl radical (DPPH) assay. The modulatory effect of GE against 2,2’-azobis(2-amidinopropane) dihydrochloride induced lipid peroxidation in rat brain regions (cortex and cerebellum) and sciatic nerve (SN) homogenates was determined. Further, the effect of GE was assessed against hyperglycemia-induced oxidative stress (OS) in SHSY5Y, a human neuroblastoma cell line.

Results: GE proved to be a good scavenger of DPPH free radical (inhibitory concentration 50% [IC50] value = 663 nmol) and could lower the lipid peroxidation levels in rat brain tissue and SN homogenates (25-40%). Further, it rescue the SHSY5Y cells from hyperglycemia-induced death. Co-exposure of GE with the IC50 level of glucose (100 mM) lowered the levels of reactive oxygen species, hydrogen peroxides and 3-nitrotyrosine levels with concomitant elevation in the glutathione levels (about two folds).

Conclusion: Collectively from these findings and other studies previously conducted (from our lab and others) emphasize the potential benefit of GE against OS, a progressive pathological feature of neurodegenerative disorders.



Geraniol, Antioxidant, In vitro studies, Diabetic neuropathy.

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Halliwell B. Oxidative stress and neurodegeneration: Where are we now? J Neurochem 2006;97(6):1634-58.

Obrosova IG. Diabetes and the peripheral. Biochem Biophys Acta 2009;10:931-40.

Kell DB. Towards a unifying, systems biology understanding of large-scale cellular death and destruction caused by poorly liganded iron: Parkinson’s, Huntington’s, Alzheimer’s, prions, bactericides, chemical toxicology and others as examples. Arch Toxicol 2010;84(11):825-89.

Kumar A, Negi G, Sharma SS. Suppression of NFkB and NFkB regulated oxidative stress and neuroinflammation by BAY11-7082 (IJB phosphorylation inhibitor) in experimental diabetic neuropathy. Biochimie 2012;94:1158-65.

Yorek MA. Treatment of diabetic neuropathy with baicalein: Intervention at multiple sites. Exp Neurol 2011;232(2):105-9.

Singh M, Arseneault M, Sanderson T, Murthy V, Ramassamy C. Challenges for research on polyphenols from foods in Alzheimer’s disease: Bioavailability, metabolism, and cellular and molecular mechanisms. J Agric Food Chem 2008;56(3):4855-73.

Le Bars PL, Katz MM, Berman N, Itil TM, Freedman AM, Schatzberg AF. A placebo-controlled, double-blind, randomized trial of an extract of Ginkgo biloba for dementia. North American EGB Study Group. JAMA 1997;278(16):1327-32.

Calabrese V, Cornelius C, Trovato A, Cavallaro M, Mancuso C, Di Rienzo L, et al. The hormetic role of dietary antioxidants in free radical-related diseases. Curr Pharm Des 2010;16(7):877-83.

Prasad SN, Muralidhara. Neuroprotective efficacy of eugenol and isoeugenol in acrylamide-induced neuropathy in rats: Behavioral and biochemical evidence. Neurochem Res 2013;38(2):330-45.

Chongtham A, Agrawal N. Curcumin modulates cell death and is protective in Huntington’s disease model. Sci Rep 2016;6:18736.

Pérez-Hernández J, Zaldívar-Machorro VJ, Villanueva-Porras D, Vega Ávila E, Chavarría A. A potential alternative against neurodegenerative diseases: Phytodrugs. Oxid Med Cell Longev 2016;2016:8378613.

Madankumar A, Jayakumar S, Gokuladhas K, Rajan B, Raghunandhakumar S, Asokkumar S, et al. Geraniol modulates tongue and hepatic phase I and phase II conjugation activities and may contribute directly to the chemopreventive activity against experimental oral carcinogenesis. Eur J Pharmacol 2013;705(1-3):148-55.

Lapczynski A, Bhatia SP, Foxenberg RJ, Letizia CS, Api AM. Fragrance material review on geraniol. Food Chem Toxicol 2008;46 Suppl 11:S160-70.

Chen W, Viljoen AM. Geraniol - A review of a commercially important fragrance material. S Afr J Bot 2010;76:643-51.

Singh BK, Tripathi M, Chaudhari BP, Pandey PK, Kakkar P. Natural terpenes prevent mitochondrial dysfunction, oxidative stress and release of apoptotic proteins during nimesulide-hepatotoxicity in rats. PLoS One 2012;7(4):e34200.

Prasad SN, Muralidhara. Protective effects of geraniol (a monoterpene) in a diabetic neuropathy rat model: Attenuation of behavioral impairments and biochemical perturbations. J Neurosci Res 2014;92(9):1205-16.

Wang J, Su B, Zhu H, Chen C, Zhao G. Protective effect of geraniol inhibits inflammatory response, oxidative stress and apoptosis in traumatic injury of the spinal cord through modulation of NF-κB and p38 MAPK. Exp Ther Med 2016;12(6):3607-13.

Kumar MA, Devaki T. Geraniol, a component of essential oils - A review of its pharmacological properties. Int J Pharm Pharm Sci 2015;7:67-70.

Tiwari M, Kakkar P. Plant derived antioxidants - Geraniol and camphene protect rat aveolar macrophages against t-BHP induced oxidative stress. Toxicol in vitro 2009;23:295-301.

Halliwell B. Are polyphenols antioxidants or pro-oxidants? What do we learn from cell culture and in vivo studies? Arch Biochem Biophys 2008;476(2):107-12.

Prasad SN, Muralidhara. Mitigation of acrylamide-induced behavioral deficits, oxidative impairments and neurotoxicity by oral supplements of geraniol (a monoterpene) in a rat model. Chem Biol Interact 2014;223:27-37.

Russo VC, Higgins S, Werther GA, Cameron FJ. Effects of fluctuating glucose levels on neuronal cells in vitro. Neurochem Res 2012;37(8):1768-82.

Chandrashekar KN, Muralidhara. Oxidative alterations induced by D-aspartic acid in prepubertal rat testis in vitro: A mechanistic study. Theriogenology 2008;70(1):97-104.

Wolff SP. Ferrous ion oxidation in the presence of ferric ion indicator xylenol orange for measurement of hydrogen peroxides. Med Enzymol 1994;233:182-9.

Mokrasch LC, Teschke EJ. Glutathione content cultured cells and rodent brain regions: Specific fluorimetric assay. Anal Biochem 1984;140:506-9.

Chandran G, Muralidhara. Neuroprotective effect of aqueous extract of Selaginella delicatula as evidenced by abrogation of rotenone-induced motor deficits, oxidative dysfunctions, and neurotoxicity in mice. Cell Mol Neurobiol 2013;33(7):929-42.

Prasad SN, Bharath MM, Muralidhara. Neurorestorative effects of eugenol, a spice bioactive: Evidence in cell model and its efficacy as an intervention molecule to abrogate brain oxidative dysfunctions in the streptozotocin diabetic rat. Neurochem Int 2016;95:24-36.

Hosamani R, Muralidhara. Prophylactic treatment with Bacopa monnieri leaf powder mitigates paraquat-induced oxidative perturbations and lethality in Drosophila melanogaster. Indian J Biochem Biophys 2010;47(2):75-82.

Ahmad ST, Arjumand W, Seth A, Nafees S, Rashid S, Ali N, et al. Preclinical renal cancer chemopreventive efficacy of geraniol by modulation of multiple molecular pathways. Toxicology 2011;290(1):69-81.

Farhath MS, Vijaya PP, Vimal M. Antioxidant activity of geraniol, geranial acetate, gingerol and eugenol. Res Pharm 2013;3:1-6.

Brownlee M. The pathobiology of diabetic complications: A unifying mechanism. Diabetes 2005;54(6):1615-25.

Pal S, Gautam S, Mishra A, Srivastava AK. Antihyperglycemic and antidyslipidemic potential of Ipomoea batatas leaves in validated diabetic model. Int J Pharm Pharm Sci 2015;7:176-86.

Vincent AM, Russell JW, Low P, Feldman EL. Oxidative stress in the pathogenesis of diabetic neuropathy. Endocr Rev 2004;25(4):612-28.

Hsu YY, Tseng YT, Lo YC. Berberine, a natural antidiabetes drug, attenuates glucose neurotoxicity and promotes Nrf2-related neurite outgrowth. Toxicol Appl Pharmacol 2013;272(3):787-96.

Laaboudi W, Ghanam J, Ghoumari O, Sounni F. Hypoglycemic and hypolipidemic effects of phenolic olive tree extract in streptozotocin diabetic rats. Int J Pharm Pharm Sci 2016;8:287-91.

Prasad SN, Muralidhara. Neuroprotective effect of geraniol and curcumin in an acrylamide model of neurotoxicity in Drosophila melanogaster: Relevance to neuropathy. J Insect Physiol 2014;60:7-16.

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Geraniol, Antioxidant, In vitro studies, Diabetic neuropathy.





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Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 7 July 2017 Page: 101-105

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Authors & Affiliations

Sathya N Prasad
Department of Biochemistry, School of Life Sciences, Vels University, Chennai, Tamil Nadu, India.

Murali Muralidhara
Department of Biochemistry and Nutrition, CSIR, Central Food Technological Research Institute, Mysuru, Karnataka, India

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