ANALYSIS OF THE ANTIOXIDANT ACTIVITY OF GERANIOL EMPLOYING VARIOUS IN-VITRO MODELS: RELEVANCE TO NEURODEGENERATION IN DIABETIC NEUROPATHY


Sathya N Prasad, Murali Muralidhara

Abstract


Objective: The aim of this study was to analyze antioxidant effect of geraniol (GE) in different in vitro models.

Methods: Initially, the antioxidant activity of GE was assessed by diphenyl picrylhydrazyl radical (DPPH) assay. The modulatory effect of GE against 2,2’-azobis(2-amidinopropane) dihydrochloride induced lipid peroxidation in rat brain regions (cortex and cerebellum) and sciatic nerve (SN) homogenates was determined. Further, the effect of GE was assessed against hyperglycemia-induced oxidative stress (OS) in SHSY5Y, a human neuroblastoma cell line.

Results: GE proved to be a good scavenger of DPPH free radical (inhibitory concentration 50% [IC50] value = 663 nmol) and could lower the lipid peroxidation levels in rat brain tissue and SN homogenates (25-40%). Further, it rescue the SHSY5Y cells from hyperglycemia-induced death. Co-exposure of GE with the IC50 level of glucose (100 mM) lowered the levels of reactive oxygen species, hydrogen peroxides and 3-nitrotyrosine levels with concomitant elevation in the glutathione levels (about two folds).

Conclusion: Collectively from these findings and other studies previously conducted (from our lab and others) emphasize the potential benefit of GE against OS, a progressive pathological feature of neurodegenerative disorders.

 


Keywords


Geraniol, Antioxidant, In vitro studies, Diabetic neuropathy.

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About this article

Title

ANALYSIS OF THE ANTIOXIDANT ACTIVITY OF GERANIOL EMPLOYING VARIOUS IN-VITRO MODELS: RELEVANCE TO NEURODEGENERATION IN DIABETIC NEUROPATHY

Keywords

Geraniol, Antioxidant, In vitro studies, Diabetic neuropathy.

DOI

10.22159/ajpcr.2017.v10i7.18564

Date

01-07-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 7 July 2017 Page: 101-105

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

169 Views | 233 Downloads

Authors & Affiliations

Sathya N Prasad
Department of Biochemistry, School of Life Sciences, Vels University, Chennai, Tamil Nadu, India.
India

Murali Muralidhara
Department of Biochemistry and Nutrition, CSIR, Central Food Technological Research Institute, Mysuru, Karnataka, India
India


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