FORMULATION AND IN-VITRO-IN-VIVO EVALUATION OF ALGINATE-CHITOSAN MICROSPHERES OF GLIPIZIDE BY IONIC GELATION METHOD
Objective: The present work is aimed to formulate and evaluate alginate-chitosan microspheres of glipizide for the effective use in the treatment of diabetes.
Methods: Sustained release microspheres were prepared by gentle mixing of polymers in water phase with drug by agitation. The polymers used for preparation were sodium alginate and chitosan, which was extruded into 5% calcium chloride solution to produce microspheres by ionic gelation method.
Results: Single unit dosage form of Glipizide causes gastric irritation. To convert it in to the multiple unit dosage form will release the drug evenly throughout the stomach which suppresses the irritation. The aim of study towards formulation and evaluation of alginate-chitosan microspheres, which can provide sustained release of the model drug. It shows better in-vitro and in-vivo activity than conventional dosage forms. The work also aims to study various parameters affecting the behavior of microspheres in oral dosage form.
Â Conclusion: Â Drugs that are simply absorbed from the gastrointestinal tract (GIT) and having a short half life are eliminated rapidly from the blood flow. To avoid this trouble, the oral sustained release (SR) has been developed as these will release the drug slowly in to the GIT and maintain a stable drug concentration in the serum for a longer period of time.
2. Sharma M, Jain K, Dev SK, Choudhury PK. Formulation and evaluation of sodium alginate beads by emulsion gelation method. Asian J Pharm 2017;11(1):S101-6.
3. Ararath D, Velmurugan S. Formulation and evaluation of nevirapine mucoadhesive microspheres. Int J Pharm Pharm Sci 2015;7:342-8.
4. Hong X, Wei L, Ma L, Chen Y, Liu Z, Yuan W. Novel preparation method for sustained-release PLGA microspheres using water-in-oil-in-hydrophilic-oil-in-water emulsion. Int J Nanomedicine 2013;8:2433-41.
5. Jorgensen L, Moeller EH, van de Weert M, Nielsen HM, Frokjaer S. Preparing and evaluating delivery systems for proteins. Eur J Pharm Sci 2006;29(3-4):174-82.
6. Castellanos IJ, Cruz G, Crespo R, Griebenow K. Encapsulation-induced aggregation and loss in activity of gamma-chymotrypsin and their prevention. J Control Release 2002;81(3):307-19.
7. Ceriello A. The emerging role of post-prandial hyperglycaemic spikes in the pathogenesis of diabetic complications. Diabet Med 1998;15(3):188-93. 8. Ministry of Health and Family Welfare. Indian Pharmacopoeia. Vol. 4. New Delhi: Controller of Publications, Government of India, Ministry of Health and Family Welfare; 2007. p. 549-50.
9. Goyal S, Rai JK, Narang RK, Rajesh KS. Sulfonyl ureas for antidiabetic therapy, an overview for glipizide - Review article. Int J Pharm Pharm Sci 2010;2 Suppl 2:1-6.
10. Deruiter J. Overview of the Antidiabetic Agents, Endocrine Pharmacotherapy Module. AQ2 ???: ???; 2003. p. 25.
11. Mishra P, Singh DN, Purohit S. Box Behnken design in optimization and evaluation of metformin HCl loaded guar gum microspheres. Invent Rapid NDDS 2012;2012(4):1-8.
12. Choudhury PK, Kar M. Preparation of alginate gel beads containing metformin hydrochloride using emulsion - Gelation method. Trop J Pharm Res 2005;4(2):489-93.
13. Choudhury PK, Kar M, Chauhan CS. Cellulose acetate microspheres as floating depot systems to increase gastric retention of antidiabetic drug: Formulation, characterization and in vitro-in vivo evaluation. Drug Dev Ind Pharm 2008;34(4):349-54.
14. Sarode SM, Mittal M, Magar RM, Shelke AD, Shrivastava B, Vidyasagar G. Formulation and evaluation of floating microspheres of glipizide. J Chem Pharm Res 2011;3(3):775-83.
15. Prisilla DH, Balamurugan R, Shah HR. Antidiabetic activity of methanol extract of Acorus calamus in STZ induced diabetic rats. Asian Pac J Trop Biomed 2012;2(2):S941-6.
16. Rajurkar BM. Phyto-pharmacological investigations of Clerodendrum infortunatum gartns. Int Res J Pharm 2011;2(11):130-2.
17. Phutane P, Shidhaye S, Lotlikar V, Ghule A, Sutar S, Kadam V. In vitro evaluation of novel sustained release microspheres of glipizide prepared by the emulsion solvent diffusion-evaporation method. J Young Pharm 2010;2(1):35-41.
18. Sinha VR, Singla AK, Wadhawan S, Kaushik R, Kumria R, Bansal K, et al. Chitosan microspheres as a potential carrier for drugs. Int J Pharm 2004;274(1-2):1-33.
19. Nair R, Haritha B, Reddy CK, Kumar A, Kumar JK. Application of chitosan microspheres as drug carriers: A review. J Pharm Sci Res 2009;1(3):1-12.
20. Longer MA, Cheng HS, Robinson JR. Mucoadhesive materials for control release dosage forms. J Pharm Sci 1987;74:406-10.
21. Mesiha M, Sidhom M. Increased oral absorption enhancement of insulin by medium viscosity hydroxyl propylcellulose. Int J Pharm 1995;115:137-40.
22. Wan LS, Heng PW, Wong LF. Matrix swelling: Simple model describing extent of swelling of HPMC matrices. Int J Pharm 1995;116(2):159-68.
23. Longer MA, Cheng HS, Robinson JR. Mucoadhesive materials for control release dosage forms. J Pharm Sci 1987;74:406-10.
24. Menshawe EL, Shahira F, Rasha MK, Doaa SH, Abdelkhalek H. Effect of biodegradable co-polymers and divalent cations on the sustained release ability of propranolol hydrochloride loaded biomaterial microspheres. Int J Pharm Pharm Sci 2016;8:311-7.
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