STRUCTURAL ELUCIDATION OF BIOACTIVE SECONDARY METABOLITES FROM ENDOPHYTIC FUNGUS

  • Shiv Kr Verma School of Biochemical Engineering IIT, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
  • Moti Lal School of Biochemical Engineering IIT, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
  • Mira Debnath Das school of biochemical engineering IIT BHU Varanasi

Abstract

Objective: In this research for bioactive secondary metabolites from endophytic fungal isolate CPR5, which was later identified and characterized as Aspergillus niger sp.

Methods: Eight bioactive compounds (1-8) were isolated and purified using a series of chromatographic techniques and identified by applying 13C and 1H nuclear magnetic resonance and mass (ESI-MS spectrometry).

Results: All the compounds were tested for antimicrobial activity against a wide range of human and plant pathogenic bacteria as well as fungi. These microorganisms were, Xanthomonas oryzae, Escherichia coli, Streptococcus pneumonia, Bacillus subtilis, Staphylococcus hyicus, Bacillus sphaericus, Staphylococcus aureus, and Pseudomonas aeruginosa, Candida albicans, Sclerotium rolfsii, Sclerotinia, scleratiourum, and Phoma exigua minimum inhibitory concentration value have also been determine for these microorganism.

Conclusion: In this study, a total 10 endophytic fungi were isolated from the Calotropis procera, show a great antibacterial activity against 24 human pathogenic bacteria.

Keywords: Endophytic fungi, Antimicrobial metabolites, Calotropis procera, Preliminary analysis of bioactive compounds and characterization.

Author Biographies

Shiv Kr Verma, School of Biochemical Engineering IIT, Banaras Hindu University, Varanasi, Uttar Pradesh, India.

Research Scholar IIT BHU Varanasi

Moti Lal, School of Biochemical Engineering IIT, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
Research Scholar IIT BHU Varanasi
Mira Debnath Das, school of biochemical engineering IIT BHU Varanasi
professor of School of Biochemical Engineering IIT BHU Varanasi

References

1. Petrini O. Fungal endophytes of tree leave. In: Andrews JA, Hirano SS, editors. Microbial Ecology of Leaves. Vol. 9. New York: Springer; 1991. p. 179-97.
2. Verma SK, Kumar A, Ashish V, Das MD. Antimicrobial activity of endophytic fungal isolate in Argemone maxicana, a traditional Indian medicinal plant. Int J Innov Res Sci Eng Technol 2014;3(3):10151-62.
3. Luiz HR, Sonia CN, Rita Q, Moraes M, Xiaoning W, Natascha TM, et al. Wedge Coniochaeta ligniaria antifungal activity of the crypticendophytic fungus associated with autotrophic tissue cultures of the Medicin plan Smallanthus sonchifolius (Asteraceae). Symbiosis 2013;60:231-8.
4. Kumaresan V, Suryanarayanan TS. Occurrence and distribution of endophytic fungi in a Mangrove community. Mycol Res 2001;105:1388-91.
5. Teng Y, Yan C, Xing X, Chuan CD. Plant symbionts, keys to the phytosphere. Symbiosis 2013;59:1-14.
6. Silva MG, Furtado NA, Pupo MT, Fonseca MJ, Said S, Silva FA. Antibacterial activity from Penicillium corylophilum Dierckx. Microbiol Res 2004;159:317-22.
7. Carroll G. Fungal endophytes in stems and leaves: From latent pathogen to mutualistic symbionts. Ecology 1988;69:2-9.
8. Gao F, Dai C, Liu X. Mechanisms of fungal endophytes in plant protection against Pathogens. Afr J Microbiol Res 2010;4(13):1346-51.
9. Schulz B, Wanke U, Draeger S, Aust HJ. Endophytes from herbaceous plants and shrubs: Effectiveness of surface sterilization methods. Mycol Res 1993;97:1447-50.
10. Amal H, Abdessamad D, Proksch P. An endophytic fungus from Nothapodytes foetida that produces camptothecin. J Nat Prod 2007;70(12):1939-45.
11. Shenxi C, Yang Z, Shubin N, Xingzhong L, Yongsheng C. Chen guanacastane diterpenoids from the plant endophytic fungus. J Nat Prod 2011;74(10):2052-61.
12. Tan RX, Zou WX. Endophytes: A rich source of functional metabolites. Nat Prod Rep 2001;18:448-59.
13. Lal M, Neelam T, Verma SK, Das MD. Studies of bioactive metabolites from endophytic bacterial isolate. Int J Sci Eng Res 2016;7(6):598-606.
14. Abraham G, Virgilio BG, Palma-Nicolás JP, Rivera G. Recent advances in antitubercular natural products. Eur J Med Chem 2012;49:1-23.
15. Stierle A, Strobel GA, Stierle D. Taxol and taxen production by Taxomyces andreanae an endophytic fungus of pacific yew. Science 1993;260:214-6.
16. Nielsen ML, Raahave JG, Justesen T. Anaerobic and aerobic skin bacteria before and after skin-disinfection with chlorhexidine: An experimental study in volunteers. J Clin Pathol 1975;28:793-7.
17. Mohamed S. Structural elucidation and bioactivity studies of secondary metabolites from endophytic Aspergillus niger. Indian J Appl Res 2015;5(9):3-7.
18. Mohamed S, Hamdi N, Amal ZH, Mohsen SA. Bioactive secondary metabolities from endophytic Aspergillus fumigates. Struct Elucidation Bioact Stud 2013;41(10):50-60.
19. Humaira N. Structural elucidation and bioactivity studies of secondary metabolites from endophytic Aspergillus niger. Indian J Appl Res 2015;5(9):3-7.
20. Jing T, Xiangjie M, Hao L, Jianglin Z, Ligang Z, Minghua Q, et al. Antimicrobial activity of sphingolipids isolated from the stems of cucumber (Cucumis sativus L.). Molecules 2016;15:9288-97.
21. Cateni F, Zilic J, Zacchigna M. Isolation and structure elucidation of cerebrosides from Euphorbia platyphyllos L. Sci Pharm 2008;76:451-69.
22. Xu J, Wang YM, Feng TY, Zhang B, Sugawara T, Xue CH. Isolation and antifatty liver activity of a novel cerebroside from the sea cucumber Acaudina molpadiodes. Biosc Biotechnol Biochem 2011;75:1466-71.
23. Larsen TO, Frydenvang K, Frisvad JC, Christophersen C. UV-guided isolation of alantrypinone, a novel Penicillium alkaloid. J Nat Prod 1998;61:1154-7.
24. Strobell G, Daisy B. Bioprospecting for microbial endophytes and their natural products. Microbiol Mol Biol Rev 2003;67:491-2.
25. Verma SK, Kumar A, Lal M, Das MD. Antimicrobial activity of extract from endophytic fungus in Calotropis procera roots Res. Environ Life Sci 2016;9(2):212-6.
Statistics
320 Views | 665 Downloads
Citatons
How to Cite
Verma, S. K., M. Lal, and M. D. Das. “STRUCTURAL ELUCIDATION OF BIOACTIVE SECONDARY METABOLITES FROM ENDOPHYTIC FUNGUS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 7, July 2017, pp. 395-00, doi:10.22159/ajpcr.2017.v10i7.18909.
Section
Original Article(s)