QUANTIFICATION AND CORRELATION OF THE BIOACTIVE PHYTOCHEMICALS OF CROTON BONPLANDIANUM LEAVES OF SUB-HIMALAYAN REGION OF WEST BENGAL
AbstractObjective: Leaves of various herbs are rich in phytochemicals which may provide protection from various diseases. Therefore, the objective of the present study was preliminary screening of the phytochemicals like tannin, phlobatannin, cholesterol, glycoside, terpinoids, phenolics, flavonoid, steroid, anthraquinone, saponin, carbohydrate, alkaloid and protein in leaf of Croton bonplandianum Baill. and quantify some of these phytochemical. Method: Standardized biochemical and UV-Vis spectrophotometric methods were followed to analyze the phytochemical status of the leaves. Principal component analysis and correlation matrix on the basis of the quantity of the bioactive phytochemicals were performed in order to elucidate the interrelation between the various phytochemicals Result: Quite a high percentage of alkaloid (59.60 Â± 4.79 g/100g), saponin (17.35 Â± 1.35 g/100g), phenolic content (75.39 Â± 3.19 mg/g), protein (55.04 Â± 2.09 mg/g), lipid (37.53 Â± 2.43 mg/g), tannin (26.18 Â± 2.63 mg/100g), thiamine (26.18Â± 2.36 mg/100g) and very satisfactory quantity of riboflavin or vitamin B2 (0.55 Â± 0.03 mg/100g), ascorbic acid (0.71 Â± 0.05 mg/100g) Â has been detected in the leaves of this plant. It is fascinating to note that the phenol with lipid and the riboflavin content have displayed almost linear positive correlation with correlation coefficient of 0.999. Conclusion: It can be concluded from the present study that the leaf of C. bonplandianum possesses rich in various phytochemicals like alkaloid, total phenol, saponin, flavonoid, protein and tannin. These phytochemicals possess various bioactive properties and may be used as external therapeutic supplement. This study may lead to a new dimension regarding the medicinal value of C. bonplandianum.
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