IN VITRO ANTIMICROBIAL ACTIVITY OF ROOT EXTRACT OF CLITORIA TERNATEA


Amita Shobha Rao, Shobha Kl, Prathibha Md’almeida, Kiranmai S Rai

Abstract


 

 Objective: Infections caused by Gram-negative bacteria are important causes of morbidity and mortality. Extracts of plants and herbs such as Clitorea ternatea are used as diuretic. This work attempts to find out antimicrobial activity of aqueous and alcoholic extract of C. ternatea roots against Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), clinical strains of Klebsiella pneumoniae, and Candida albicans.

Methods: The agar well-diffusion method was done using Mueller Hinton agar and Sabouraud’s dextrose agar. The microorganism grown in peptone water was inoculated into culture medium. 4 mm diameter well punched into the agar was filled with 20 μl of aqueous and alcoholic root extracts C. ternatea extracts in various concentrations (100-25 μg/ml). The plates were incubated and antimicrobial activity was evaluated.

Results: Aqueous root extract of C. ternatea with the concentration of 100 μg/ml showed zone of inhibition against E. coli (ATCC 25922) 18 mm, P. aeruginosa (ATCC 27853) 14 mm, multidrug resistant strain of K. pneumoniae 15 mm. Alcoholic extract of C. ternatea with the concentration of 100 μg/ml showed zone of inhibition of 35 mm against E. coli (ATCC 25922), P. aeruginosa (ATCC 27853) 22 mm, and multidrug resistant strain of K. pneumoniae 28 mm. C. albicanswas resistant to both extract of C. ternatea root.

Conclusions: Alcoholic extract of C. ternatea is a better antibacterial agent against multidrug resistant Klebsiella species and other Gram-negative pathogens. Further, studies are required to identify active substances from the alcoholic extracts of C. ternatea for treating infections.


Keywords


Clitorea ternatea, Antimicrobial sensitivity, Multidrug resistant.

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About this article

Title

IN VITRO ANTIMICROBIAL ACTIVITY OF ROOT EXTRACT OF CLITORIA TERNATEA

Keywords

Clitorea ternatea, Antimicrobial sensitivity, Multidrug resistant.

DOI

10.22159/ajpcr.2017.v10i11.19992

Date

01-11-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 11 November 2017 Page: 52-54

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

37 Views | 82 Downloads

Authors & Affiliations

Amita Shobha Rao
Department of Microbiology, Melaka Manipal Medical College, Manipal University, Manipal, Karnataka, India.
India

Shobha Kl
Department of Microbiology, Melaka Manipal Medical College, Manipal University, Manipal, Karnataka, India.
India

Prathibha Md’almeida
Department of Physiology, Melaka Manipal Medical College, Manipal University, Manipal, Karnataka, India.
India

Kiranmai S Rai
Department of Physiology, Melaka Manipal Medical College, Manipal University, Manipal, Karnataka, India.
India


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