IN VITRO ANTIMICROBIAL ACTIVITY OF ROOT EXTRACT OF CLITORIA TERNATEA
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i11.19992Keywords:
Clitorea ternatea, Antimicrobial sensitivity, Multidrug resistantAbstract
Â
 Objective: Infections caused by Gram-negative bacteria are important causes of morbidity and mortality. Extracts of plants and herbs such as Clitorea ternatea are used as diuretic. This work attempts to find out antimicrobial activity of aqueous and alcoholic extract of C. ternatea roots against Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), clinical strains of Klebsiella pneumoniae, and Candida albicans.
Methods: The agar well-diffusion method was done using Mueller Hinton agar and Sabouraud's dextrose agar. The microorganism grown in peptone water was inoculated into culture medium. 4 mm diameter well punched into the agar was filled with 20 μl of aqueous and alcoholic root extracts C. ternatea extracts in various concentrations (100-25 μg/ml). The plates were incubated and antimicrobial activity was evaluated.
Results: Aqueous root extract of C. ternatea with the concentration of 100 μg/ml showed zone of inhibition against E. coli (ATCC 25922) 18 mm, P. aeruginosa (ATCC 27853) 14 mm, multidrug resistant strain of K. pneumoniae 15 mm. Alcoholic extract of C. ternatea with the concentration of 100 μg/ml showed zone of inhibition of 35 mm against E. coli (ATCC 25922), P. aeruginosa (ATCC 27853) 22 mm, and multidrug resistant strain of K. pneumoniae 28 mm. C. albicanswas resistant to both extract of C. ternatea root.
Conclusions: Alcoholic extract of C. ternatea is a better antibacterial agent against multidrug resistant Klebsiella species and other Gram-negative pathogens. Further, studies are required to identify active substances from the alcoholic extracts of C. ternatea for treating infections.
Downloads
References
Bhalodia NR, Shukla VJ. Antibacterial and antifungal activities from leaf extracts of Cassia fistulal: An ethnomedicinal plant. J Adv Pharm Technol Res 2011;2(2):104-19.
Al-Snafi AE. Pharmacological importance of Clitoria ternatea. A review. IOSR J Pharm 2016;6(3):68-83.
Al-Snafi AE. Chemical constituents and pharmacological importance of Agropyronrepens. A review. Res J Pharmacol Toxicol 2015;1(2):37-41.
Ponnusamy S, Gnanaraj WE, Marimuthu J, Selvakumar V, Nelson J. The effect of leaves extracts of Clitoria ternatea Linn against the fish pathogens. Asian Pac J Trop Med 2010;3(9):723-6.
Dixit A, Singh H, Sharma RA, Sharma A. Estimation of antioxidant and antibacterial activity of crude extracts of Thevetia peruviana (pers.) K. Schum. Int J Pharm Pharm Sci 2015;7(2):55-9.
Bushra Beegum NR, Devi TG. Antibacterial activity of selected seaweeds from Kovalam south West coast of India. Asian J Microbiol Biotechnol Environ Exp Sci 2003;5(3):319-22.
Kamsala RV, Lepakshi BM, Padma Y, Venkata Raju RR. Studies on antimicrobial and antioxidant properties of leaf extracts of Syzygium alternifolium (wt.) walp. Int J Pharm Pharm Sci 2015;7(2):139-43.
Sivaranjan VV, Balachandran I. Ayuruedic Drugs and Their Plant Sources. New Delhi: Oxford & IBM; 1994. p. 425-8.
Devi BP, Boominathan R, Mandal SC. Anti-inflammatory, analgesic and antipyretic properties of Clitoria ternatea root. Fitoterapia 2003;74(4):345-9.
Mhaskar AV, Prakash K, Vishwakarma KS, Maheshwari VL. Callus induction and antimicrobial activity of seed and callus extracts of Clitoria ternatea L. Curr Trends Biotechnol Pharm 2010;3(4):561-7.
Rai KS, Murthy KD, Karanth KS, Rao MS. Clitoria ternatea (Linn) root extract treatment during growth spurt period enhances learning and memory in rats. Indian J Physiol Pharmacol 2001;45(3):305-13.
Shakya VK, Saxena S, Shakya A. Effect of ethanolic extract of Allium sativum bulbs on streptozotocinet induced diabetic rats. J Chem Pharm Res 2010;2(6):171-5.
Rao AS, Nayanatara AK, Kaup SR, Sharma A, Kumar BA, Bhavesh D, et al. Potential antibacterial and antifungal activity of aqueous extract of Cynodon dactylon. Int J Pharm Sci Res 2011;2(11):2889-93.
Achika JI, Ndukwe GI, Ayo RG. Phytochemical screening and antimicrobial studies of aerial part of Aeschynomene uniflora Mey. Ind Chem 2016;2:113.
Rates SM. Plants as source of drugs. Toxicon 2001;39(5):603-13.
Haripriya D, Selvan N, Jeyakumar N, Periasamy R, Johnson M, Irudayaraj V. The effect of extracts of Selaginella involvens and Selaginella inaequalifolia leaves on poultry pathogens. Asian Pac J Trop Med 2010;3(9):723-6.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
