CENTRAL COMPOSITE DESIGN TO DEVELOP A ROBUST REVERSED-PHASE THIN-LAYER CHROMATOGRAPHY/DENSITOMETRY METHOD FOR QUANTIFICATION OF PARABENS IN COSMETIC PREPARATIONS

  • Kavitha Jayaseelan Department of Pharmaceutical Analysis, SRM College of Pharmacy, SRM University, Kattankulathur, Kancheepuram - 603 203, Tamil Nadu, India.
  • Karunanidhi Santhana Lakshmi Department of Pharmaceutical Analysis, SRM College of Pharmacy, SRM University, Kattankulathur, Kancheepuram - 603 203, Tamil Nadu, India.

Abstract

 

 Objective: The current study was framed with a view to develop a simple, sensitive and a rapid reversed-phase thin-layer chromatography (RP-TLC)/ densitometry method for quantification of parabens (methyl, ethyl, propyl, and butyl) in various cosmetic preparations available in local market. Moreover, robustness testing was planned to be performed applying a central composite design (CCD).

Methods: The desired chromatographic separation was achieved on RP-TLC/densitometry plates precoated with Silica gel 60 GF254 on aluminum sheet, employing methanol:toluene:acetonitrile:glacial acetic acid as mobile phase in the ratio of 3:5:1.8:0.2 v/v/v/v and detection was carried out at 256 nm.

Results: The mobile phase used gave an excellent resolution of all the four separated parabens. The method was found linear over a wide range of 6.25- 400 ng/spot. The optimized method was validated by measuring various validation parameters. CCD was employed to check the robustness of the method at three-factor levels. The developed and optimized method was used to quantify parabens in 30 different cosmetic products procured from local market.

Conclusion: The proposed and developed RP-TLC/densitometry method can be applied for routine analysis of all the parabens in cosmetic products and can also be extended to the analysis in pharmaceuticals and food products.

Keywords: Central composite design, Cosmetic preparations, Parabens, Reversed-phase thin-layer chromatography/densitometry, Validation.

Author Biography

Kavitha Jayaseelan, Department of Pharmaceutical Analysis, SRM College of Pharmacy, SRM University, Kattankulathur, Kancheepuram - 603 203, Tamil Nadu, India.
PHARMACEUTICAL ANALYSIS, LECTURER

References

1. Haag T, Loncrini DF. Esters of para-hydroxybenzoic acid. Cosmet Sci Technol Serv 1984;1:63-77.
2. Vo TT, Yoo YM, Choi KC, Jeung EB. Potential estrogenic effect(s) of parabens at the prepubertal stage of a postnatal female rat model. Reprod Toxicol 2010;29(3):306-16.
3. Boberg J, Taxvig C, Christiansen S, Hass U. Possible endocrine disrupting effects of parabens and their metabolites. Reprod Toxicol 2010;30(2):301-12.
4. Oishi S. Effects of propyl paraben on the male reproductive system. Food Chem Toxicol 2002;40(12):1807-13.
5. Oishi S. Effects of butyl paraben on the male reproductive system in mice. Arch Toxicol 2002;76(7):423-9.
6. Ambekar AM, Kuchekar BS. Application of a validated stability-indicating HPTLC method for simultaneous quantitative determination of candesartan cilexetil and hydrochlorothiazide in pharmaceutical dosage form. Int J Pharm Pharm Sci 2016;8(5):151-7.
7. Kaul N, Agrawal H, Paradkar AR, Mahadik KR. HPTLC method for determination of nevirapine in pharmaceutical dosage form. Talanta 2004;62(4):843-52.
8. Swarbrick J. Encyclopedia of Pharmaceutical Technology. 1st ed., Vol. 1. London, UK: Informa Healthcare Inc.; 2007. p. 2452-67.
9. Ravichandiran V, Sumithra M. Application of quality by design (CCD technique) for simultaneous estimation of cefixime and ofloxacin by HPTLC method. Int J Pharm Pharm Sci 2016;8(7):200-28.
10. Uysal UD, Guray T. Determination of parabens in pharmaceutical and cosmetic products by capillary electrophoresis. J Anal Chem 2008;63:982-6.
11. Noguera-Orti JF, Villanueva-Camanas RM, Ramis-Ramos G. Determination of parabens in cosmetics without previous extraction by micellar liquid chromatography. J Chromatogr Sci 1999;37:83-7.
12. Lokhnauth JK, Snow NH. Determination of parabens in pharmaceutical formulations by solid-phase microextraction-ion mobility spectrometry. Anal Chem 2005;77(18):5938-46.
13. Hao YS, Hai LJ, Hong LM, Gang P, Lu Z, Yun FC. Simultaneous determination of seven phthalates and four parabens in cosmetic products using HPLC-DAD and GC-MS methods. J Sep Sci 2007;30:48-54.
14. Grosa G, Del Grosso E, Russo R, Allegrone G. Simultaneous, stability indicating, HPLC-DAD determination of guaifenesin and methyl and propyl-parabens in cough syrup. J Pharm Biomed Anal 2006;41(3):798-803.
15. Canosa P, Perez-Palacios D, Garrido-Lopez A, Tena MT, Rodriguez I, RubI E, et al. Pressurized liquid extraction with in-cell clean-up followed by gas chromatography-tandem mass spectrometry for the selective determination of parabens and triclosan in indoor dust. J Chromatogr A 2007;1161:105-12.
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Jayaseelan, K., and K. S. Lakshmi. “CENTRAL COMPOSITE DESIGN TO DEVELOP A ROBUST REVERSED-PHASE THIN-LAYER CHROMATOGRAPHY/DENSITOMETRY METHOD FOR QUANTIFICATION OF PARABENS IN COSMETIC PREPARATIONS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 10, Sept. 2017, pp. 230-4, doi:10.22159/ajpcr.2017.v10i10.20160.
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