• Srimathi Priyanga K Department of Biochemistry, Bharathi Women’s College, Chennai - 600 108, Tamil Nadu, India
  • Vijayalakshmi K Department of Biochemistry, Bharathi Women’s College, Chennai - 600 108, Tamil Nadu, India



Quercetin, Hesperidin, 1, 1-diphenyl-2-picrylhydrazyl, Radical scavenging activity



 Objective: The study was carried out to evaluate the antioxidant potential of quercetin and hesperidin.

Methods: The antioxidant potential of quercetin and hesperidin was estimated using free radicals in in vitro condition. Antioxidant activity of quercetin and hesperidin was estimated using 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide, nitric oxide, hydroxyl radical, hydrogen peroxide radical, and reducing power assay.

Results: Quercetin and hesperidin have high efficiency in scavenging free radicals. Both the compounds are more efficient in scavenging DPPH (IC50 value 36.15±0.30 and 41.55±0.49 mg/ml) and superoxide radicals (IC 50 value 19.3±0.26 and 28.08 ±0.18 mg/ml) have high reducing capacity when compared to the standards ascorbic acid (IC50 value 31.45±1.31 and 23.2±0.20 mg/ml).

Conclusion: The present study suggests that quercetin and hesperidin have significant antioxidant activities and could be easily accessible to the immune system.


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Halliwell B. Free radicals and antioxidants: A personal view. Nutr Rev 1994;52:253-65.

Newman DJ, Cragg GM, Snader KM. Natural products as sources of new drugs over the period 1981-2002. J Nat Prod 2003;66(7):1022-37.

Chandran RP, Kalaiselvi M, Bhuvaneshwari V, Amsaveni R, Ragavendran P. In vitro assessment of free radical scavenging activity of Jasminum grandflorum (L) flower. Asian J Pharm Clin Res 2016;9(1):171-4.

Grace PA. Ischemia-reperfusion-injuring. Br J Surg 1994;81:637-47.

Bahorun T, Soobrattee MA, Luximon-Rammav V. Free radicals and antioxidants in cardiovascular health and disease. Internet J Med Update 2006;1(2). Available from: 05_jul-dec 2006.htm.

Santos AC, Uyemura SA, Lopes JL, Bazon JN, Mingatto FE, Curti C. Effect of naturally occurring flavonoids on lipid peroxidation and membrane permeability transition in mitochondria. Free Radic Biol Med 1998;24(9):1455-61.

Kerry NL, Abbey M. Red wine and fractionated phenolic compounds prepared from red wine inhibits low density lipoprotein oxidation in vitro. Atherosclerosis 1997;135:93-102.

Shoskes DA. Effect of bioflavonoids quercetin and curcumin on ischemic renal injury: A new class of renoprotective agents. Transplantation 1998;66(2):147-52.

Shutenko Z, Henry Y, Pinard E, Seylaz J, Potier P, Berthet F, et al. Influence of the antioxidant quercetin in vivo on the level of nitric oxide determined by electron paramagnetic resonance in rat brain during global ischemia and reperfusion. Biochem Pharmacol 1999;57(2):199-208.

Choi YJ, Kang JS, Park JH. Polyphenolic flavonoids differ in their antiapoptotic. Newman DJ, Cragg GM, Snader KM. Natural products as sources of new drugs over the period 1981–2002. J Nat Prod 2003; 66:1022-37.

Rice-Evans CA, Miller NJ, Bolwell PG, Bramley PM, Pridham JB. The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radic Res 1995;22(4):375-83.

Ferrali M, Signorini C, Ciccoli L, Bambagioni S, Rossi V, Pompella A, et al. Protection of erythrocytes against oxidative damage and autologous immunoglobulin G (IgG) binding by iron chelator fluor-benzoil-pyridoxal hydrazone. Biochem Pharmacol 2000;59(11):1365-73.

Crozier A, Jaganath IB, Clifford MN. Dietary phenolics chemistry, bioavailability and effects on health. Nat Prod Rep 2009;26(8):1001-43.

Chen MC, Ye YY, Ji G, Liu JW. Hesperidin upregulates heme oxygenase-1 to attenuate hydrogen peroxide-induced cell damage in hepatic L02 cells. J Agric Food Chem 2010;58(6):3330-5.

Cho J. Antioxidant and neuroprotective effects of hesperidin and its aglycone hesperetin. Arch Pharm Res 2006;29(5):699-706.

Mensor LL, Menezes FS, Leitace GG, Reis AS, Dos Santos TC, Coube CS, et al. Screening of Braziliam plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother 2001;15:127-30.

Liu F, Ooi VE, Chang ST. Free radical scavenging activities of mushroom polysaccharide extracts. Life Sci 1997;60(10):763-71.

Govindarajan R, Rastogi S, Vijayakumar M, Shirwaikar A, Rawat AK, Mehrotra S, et al. Studies on the antioxidant activities of Desmodium gangeticum. Biol Pharm Bull 2003;26(10):1424-7.

Halliwell B, Gutteridge JM, Aruoma OI. The deoxyribose method: A simple ‘test-tube’ assay for determination of rate constants for reactions of hydroxyl radicals. Anal Biochem 1987;165(1):215-9.

Ruch R, Cheng S, Klanning J. Prevention of cytotoxicity and inhibition of inter cellular communication antioxidant catechins isolated from Chinese green tea. Carcinogenesis 1989;10:1003-8.

Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Jpn J Nutr 1986;44:307-15.

Stratil P, Klejdus B, Kubán V. Determination of phenolic compounds and their antioxidant activity in fruits and cereals. Talanta 2007;71(4):1741-51.

Kalaivani T, Mathew L. Free radical scavenging activity from leaves of Acacia nilotica (L.) Wild. Ex Delile, an Indian medicinal tree. Food Chem Toxicol 2010;48(1):298-305.

Halliwell B, Gutteridge JM. Free Radicals in Biology and Medicine. 4th ed. Oxford: Oxiford University Press; 2007.

Esmaeili MA, Sonboli A. Antioxidant, free radical scavenging activities of Salvia brachyantha and its protective effect against oxidative cardiac cell injury. Food Chem Toxicol 2010;48(3):846-53.

Hofseth LJ. Nitric oxide as a target of complementary and alternative medicines to prevent and treat inflammation and cancer. Cancer Lett 2008;268(1):10-30.

Sulaiman SF, Yusoff NA, Eldeeri IM, Seow EM, Sajak AA, Supriatno OK. Correlation between total phenolic and mineral contents with antioxidant activity of eight Malaysian bananas (Musa. sp). J Food Compos Anal 2011;24:1-10.

Mahadevan MU, Ramaswamy RS, Banumathi V. Mimosa pudica exerts neuroprotection against MPP+ induced neurotoxicity SHSY5Y cell lines-an in vitro model of anti-parkinsonism. Int J Pharm Pharm Sci 2017;9(1):21-6.

Rex DA. Selenium enriched mushrooms as a food supplement for prevention of neurodegenerative diseases. Int J Pharm Pharm Sci 2014;10(6):1-2.



How to Cite

Priyanga K, S., and V. K. “INVESTIGATION OF ANTIOXIDANT POTENTIAL OF QUERCETIN AND HESPERIDIN: AN IN VITRO APPROACH”. Asian Journal of Pharmaceutical and Clinical Research, vol. 10, no. 11, Nov. 2017, pp. 83-86, doi:10.22159/ajpcr.2017.v10i11.20260.



Original Article(s)