INVESTIGATION OF ANTIOXIDANT POTENTIAL OF QUERCETIN AND HESPERIDIN: AN IN VITRO APPROACH
Keywords:Quercetin, Hesperidin, 1, 1-diphenyl-2-picrylhydrazyl, Radical scavenging activity
Â Objective: The study was carried out to evaluate the antioxidant potential of quercetin and hesperidin.
Methods: The antioxidant potential of quercetin and hesperidin was estimated using free radicals in in vitro condition. Antioxidant activity of quercetin and hesperidin was estimated using 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide, nitric oxide, hydroxyl radical, hydrogen peroxide radical, and reducing power assay.
Results: Quercetin and hesperidin have high efficiency in scavenging free radicals. Both the compounds are more efficient in scavenging DPPH (IC50 value 36.15Â±0.30 and 41.55Â±0.49 mg/ml) and superoxide radicals (IC 50 value 19.3Â±0.26 and 28.08 Â±0.18 mg/ml) have high reducing capacity when compared to the standards ascorbic acid (IC50 value 31.45Â±1.31 and 23.2Â±0.20 mg/ml).
Conclusion: The present study suggests that quercetin and hesperidin have significant antioxidant activities and could be easily accessible to the immune system.
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