A RAPID AND SENSITIVE LIQUID CHROMATOGRAPHY- MASS SPECTROMETRY/MASS SPECTROMETRY METHOD FOR ESTIMATION OF PIOGLITAZONE, KETO PIOGLITAZONE AND HYDROXY PIOGLITAZONE IN HUMAN PLASMA


Revathi Naga Lakshmi Ponnuri, Prahlad Pragallapati, Ravindra N, Venkata Basaveswara Rao Mandava

Abstract


 

 Objective: The main objective of the work was to develop a straightforward, fast and selective liquid chromatography/tandem mass spectrometry (LC-MS/MS) assay for determination of pioglitazone (PG), keto pioglitazone (KPG), and hydroxy pioglitazone (HPG) in human plasma and to validate as per recent guidelines.

Methods: Analyte and the internal standard (IS) were extracted from plasma through liquid-liquid extraction and chromatographed on a Xterra RP18, 100×4.6, 5 μ column using methanol: acetonitrile mixture and 10 mM Ammonium formate buffer (70:30, v/v) as the mobile phase at a flow rate of 0.7 mL/min. The API-3200 Q Trap LC-MS/MS instrument in multiple reaction monitoring mode was used for detection. Diphenhydramine was utilized as IS.

Results: The linearity was established in the concentration range of 20.15-1007.58 ng/mL for PG, 20.35-1017.58 ng/mL for KPG, and 19.68-491.22 ng/mL for HPG in human plasma. All the validation parameters were well within the acceptance limits.

Conclusion: A new simple LC-MS/MS method was developed for the determination of PG, KPG, and HPG in human plasma. This method can be easily applied for the estimation of pharmacokinetic parameters of PG, KPG, and HPG.


Keywords


Pioglitazone, Keto pioglitazone, Hydroxy pioglitazone, Human plasma, Method validation, Liquid chromatography/tandem mass spectrometry.

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References


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About this article

Title

A RAPID AND SENSITIVE LIQUID CHROMATOGRAPHY- MASS SPECTROMETRY/MASS SPECTROMETRY METHOD FOR ESTIMATION OF PIOGLITAZONE, KETO PIOGLITAZONE AND HYDROXY PIOGLITAZONE IN HUMAN PLASMA

Keywords

Pioglitazone, Keto pioglitazone, Hydroxy pioglitazone, Human plasma, Method validation, Liquid chromatography/tandem mass spectrometry.

DOI

10.22159/ajpcr.2017.v10i12.20284

Date

01-12-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 12 December 2017 Page: 120-128

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

18 Views | 43 Downloads

Authors & Affiliations

Revathi Naga Lakshmi Ponnuri
Department of Pharmacy, Faculty Pharmaceutical Sciences, Pharmaceutical Analysis, Krishna University, Machilipatnam - 521 002, Andhra Pradesh, India.
India

Prahlad Pragallapati
Department of Pharmaceutics, Acharya Nagarjuna University, Guntur - 522 510, Andhra Pradesh, India.
India

Ravindra N
Department of Pharmaceutical Chemistry, Chilkur Balaji College of Pharmacy, Hyderabad - 500 075, Telangana, India.
India

Venkata Basaveswara Rao Mandava
Department of Chemistry, Faculty of Sciences, Directorate of Admissions, Krishna University, Machilipatnam - 521 002, Andhra Pradesh, India.
India


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