A RAPID AND SENSITIVE LIQUID CHROMATOGRAPHY- MASS SPECTROMETRY/MASS SPECTROMETRY METHOD FOR ESTIMATION OF PIOGLITAZONE, KETO PIOGLITAZONE AND HYDROXY PIOGLITAZONE IN HUMAN PLASMA

Revathi Naga Lakshmi Ponnuri; Prahlad Pragallapati; Ravindra N; Venkata Basaveswara Rao Mandava

doi:10.22159/ajpcr.2017.v10i12.20284

Authors

Revathi Naga Lakshmi Ponnuri Department of Pharmacy, Faculty Pharmaceutical Sciences, Pharmaceutical Analysis, Krishna University, Machilipatnam - 521 002, Andhra Pradesh, India.
Prahlad Pragallapati Department of Pharmaceutics, Acharya Nagarjuna University, Guntur - 522 510, Andhra Pradesh, India.
Ravindra N Department of Pharmaceutical Chemistry, Chilkur Balaji College of Pharmacy, Hyderabad - 500 075, Telangana, India.
Venkata Basaveswara Rao Mandava Department of Chemistry, Faculty of Sciences, Directorate of Admissions, Krishna University, Machilipatnam - 521 002, Andhra Pradesh, India.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i12.20284

Keywords:

Pioglitazone, Keto pioglitazone, Hydroxy pioglitazone, Human plasma, Method validation, Liquid chromatographytandem mass spectrometry

Abstract

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Â Objective: The main objective of the work was to develop a straightforward, fast and selective liquid chromatography/tandem mass spectrometry (LC-MS/MS) assay for determination of pioglitazone (PG), keto pioglitazone (KPG), and hydroxy pioglitazone (HPG) in human plasma and to validate as per recent guidelines.

Methods: Analyte and the internal standard (IS) were extracted from plasma through liquid-liquid extraction and chromatographed on a Xterra RP18, 100Ã—4.6, 5 Î¼ column using methanol: acetonitrile mixture and 10 mM Ammonium formate buffer (70:30, v/v) as the mobile phase at a flow rate of 0.7 mL/min. The API-3200 Q Trap LC-MS/MS instrument in multiple reaction monitoring mode was used for detection. Diphenhydramine was utilized as IS.

Results: The linearity was established in the concentration range of 20.15-1007.58 ng/mL for PG, 20.35-1017.58 ng/mL for KPG, and 19.68-491.22 ng/mL for HPG in human plasma. All the validation parameters were well within the acceptance limits.

Conclusion: A new simple LC-MS/MS method was developed for the determination of PG, KPG, and HPG in human plasma. This method can be easily applied for the estimation of pharmacokinetic parameters of PG, KPG, and HPG.

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