IN VITRO ANTIOXIDANT AND ANTIHYPERGLYCEMIC ACTIVITIES OF HYDROETHANOLIC LEAF EXTRACT OF ACACIA CATECHU (L.F) WILLD
Â Objective: The aim of this study was to analyze the antioxidant and antihyperglycemic activities of hydroethanolic leaf extract of Acacia catechu (L.f) Willd.
Methods: The A. catechu (L.f) Willd. leaf extract was subjected to antioxidant activities such as 1,1-diphenyl-1-picryl hydrazyl (DPPH), hydroxyl radical (OHâˆ’), nitric oxide radical (NOâˆ’), and superoxide radical (SO) scavenging assays. Further, the leaf extract was subjected to antihyperglycemic activity by Î±-amylase inhibition assay.
Results: The free radical scavenging activity of the plant extract was found to increase with increase in concentration (20-100 Î¼g/ml) and the maximum activity was obtained at 100 Î¼g/ml, which was compared with the standard ascorbic acid. The percentage inhibition of free radicals was found to be 62.94Â±0.91%, 63.53Â±1.08%, 71.04Â±0.48%, and 51.50Â±0.86% for DPPH, OHâˆ’, NOâˆ’, and SOâˆ’, respectively. The IC50 for DPPH, OHâˆ’, NOâˆ’, and SO were recorded as 73.81Â±0.95, 69.03Â±0.58, 60.74Â±0.37, and 83.10Â±1.00 Î¼g/ml. The IC50 value of Î±-amylase inhibitory activity was found to be 71.17 Î¼g/ml.
Conclusion: The present investigations suggest that the A. catechu (L.f) Willd may serve as a potential source of natural antioxidant and antihyperglycemic agent that could have great importance in pharmaceutical preparations.
2. Young IS, Woodside JV. Antioxidants in health and disease. J Clin Pathol 2001;54(3):176-86.
3. Santhoshkumar M, Anusuya N, Bhuvaneswari P. Antiulcerogenic effect of resin from shorea robusta Gaertn F on experimentally induced ulcer models. Int J Pharm Pharm Sci 2012;5(1):269-72.
4. Prasad DN, Rao BG, Sambasivarao E, Mallikarjunarao T, Praneeth D. Quantification of constituents and In-vitro antioxidant activity of Ficus semicordata leaves extracts. Int J Pharm Pharm Sci 2012;4(2):619-22.
5. Sudhanshu, Nidhi R, Sandhya M, Vishal, Ekta M. Antioxidant agents alternative source for malaria disease. Int J Appl Pharm 2012;4(2):14-6.
6. Betteridge DJ. What is oxidative stress? Metabolism 2000;49 2 Suppl 1:3-8.
7. Lonkar P, Dedon PC. Reactive species and DNA damage in chronic inflammation: Reconciling chemical mechanisms and biological fates. Int J Cancer 2011;128(9):1999-2009.
8. Subramanian R, Asmawi MZ, Sadikun A. In vitro alpha-glucosidase and alpha-amylase enzyme inhibitory effects of Andrographis paniculata extract and andrographolide. Acta Biochim Pol 2008;55(2):391-8.
9. Vaishali VA, Sangeeta SM, Mandar A, Kishore MP. Antioxidant and trace element potential of chyavanprash and some ayurvedic preparations. Indian J Tradit Knowl 2003;2:215-23.
10. Stohs SJ, Bagchi D. Antioxidant, anti-inflammatory, and chemoprotective properties of acacia catechu heartwood extracts. Phytother Res 2015;29(6):818-24.
11. Brand-Williams W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. Lebensm Wiss Technol 1995;28(1):25-30.
12. Halliwell B, Gutteridge JM, Aruoma OI. The deoxyribose method: A simple â€˜test-tubeâ€™ assay for determination of rate constants for reactions of hydroxyl radicals. Anal Biochem 1987;165(1):215-9.
13. Sreejayan N, Rao MN. Nitric oxide scavenging by curcuminoids. J Pharm Pharmacol 1997;49(1):105-7.
14. Winterbourn CC, Hawkins RE, Brian M, Carrell RW. The estimation of red cell superoxide dismutase activity. J Lab Clin Med 1975;85(2):337-41.
15. Narkhede MB, Ajimire PV, Waugh E, Manoj M, Shivashanmugam AT.
In-vitro antidiabetic activity of Caesalpinia digyna (R.) methanol root extract. Asian J Plant Sci Res 2011;1(2):101-6.
16. Liu F, Ooi VE, Chang ST. Free radical scavenging activity of mushroom polysaccharide extracts. Life Sci 1997;60(10):763-71.
17. Gutteridge JM. Reactivity of hydroxyl and hydroxyl-like radicals discriminated by release of thiobarbituric acid-reactive material from deoxy sugars, nucleosides and benzoate. Biochem J 1984;224(3):761-7.
18. Spencer JP, Jenner A, Aruoma OI, Evans PJ, Kaur H, Dexter DT, et al. Intense oxidative DNA damage promoted by L-dopa and its metabolites. Implications for neurodegenerative disease. FEBS Lett 1994;353(3):246-50.
19. Wang H, Gao X, Zhou G, Cai L, Yao WB. In-vitro and in-vivo antioxidant activity of aqueous extract from Choerospondias axillaris fruit. Food Chem 2008;106(3):888-95.
20. Reddy BS, Reddy RK, Reddy BP, Ramakrishna S, Diwan PV. Potential in-vitro antioxidant and protective effects of Soymida febrifuga on ethanol induced oxidative damage in HepG2 cells. Food Chem Toxicol 2008;46(11):3429-42.
21. Chaulya NC, Haldar PK, Mukherjee A. In-vitro free radical scavenging activity of methanol extract of rhizome of Cyperus tegetum Roxb (Cyperaceae). Int J Curr Pharm Res 2010;2(3):39-43.
22. Halliwell B, Gutteridge JM. Free Radicals in Biology and Medicine. 4th ed. Oxford, UK: Oxford University Press; 2007.
23. Gally HF, Howdle PD, Walker BE, Webster NR. The effects of intravenous antioxidants in patients with septic shock. Free Radic Biol Med 1997;23(5):768-74.
24. Matsui T, Ueda T, Oki T, Sugita K, Terahara N, Matsumoto K. Alpha-glucosidase inhibitory action of natural acylated anthocyanins 1. Survey of natural pigments with potent inhibitory activity. J Agric Food Chem 2001;49(4):1948-51.
25. Fariba S, Gholamreza DN, Mansour M. Major flavonoids with antioxidant activity from Teucrium polium L. Food Chem 2009;112(4):885-8.
26. Prabhakar VK, Jaidka A, Singh R. In-vitro study on Î±-amylase inhibitory activity and phytochemical screening of few Indian medicinal plant having anti-diabetic properties. Int J Sci Res Publ 2013;3(8):1-6.
27. Tarling CA, Woods K, Zhang R, Brastianos HC, Brayer GD, Andersen RJ, et al. The search for novel human pancreatic alpha-amylase inhibitors: High-throughput screening of terrestrial and marine natural product extracts. Chembiochem 2008;9(3):433-8.
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