IN VITRO ANTIOXIDANT AND ANTIHYPERGLYCEMIC ACTIVITIES OF HYDROETHANOLIC LEAF EXTRACT OF ACACIA CATECHU (L.F) WILLD

  • Ramesh Balu Department of Biochemistry, PSG College of Arts & Science, Coimbatore, Tamil Nadu, India. Email
  • Jayabharathi V Department of Biochemistry, PSG College of Arts & Science, Coimbatore, India.

Abstract

 

 Objective: The aim of this study was to analyze the antioxidant and antihyperglycemic activities of hydroethanolic leaf extract of Acacia catechu (L.f) Willd.

Methods: The A. catechu (L.f) Willd. leaf extract was subjected to antioxidant activities such as 1,1-diphenyl-1-picryl hydrazyl (DPPH), hydroxyl radical (OH−), nitric oxide radical (NO−), and superoxide radical (SO) scavenging assays. Further, the leaf extract was subjected to antihyperglycemic activity by α-amylase inhibition assay.

Results: The free radical scavenging activity of the plant extract was found to increase with increase in concentration (20-100 μg/ml) and the maximum activity was obtained at 100 μg/ml, which was compared with the standard ascorbic acid. The percentage inhibition of free radicals was found to be 62.94±0.91%, 63.53±1.08%, 71.04±0.48%, and 51.50±0.86% for DPPH, OH−, NO−, and SO−, respectively. The IC50 for DPPH, OH−, NO−, and SO were recorded as 73.81±0.95, 69.03±0.58, 60.74±0.37, and 83.10±1.00 μg/ml. The IC50 value of α-amylase inhibitory activity was found to be 71.17 μg/ml.

Conclusion: The present investigations suggest that the A. catechu (L.f) Willd may serve as a potential source of natural antioxidant and antihyperglycemic agent that could have great importance in pharmaceutical preparations.

 

Keywords: Acacia catechu (L.f) Willd, 1, 1-diphenyl-1-picryl hydrazyl, Hydroxyl radical, Nitric oxide radical, Superoxide oxide radical, α-amylase inhibition.

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How to Cite
Balu, R., and J. V. “IN VITRO ANTIOXIDANT AND ANTIHYPERGLYCEMIC ACTIVITIES OF HYDROETHANOLIC LEAF EXTRACT OF ACACIA CATECHU (L.F) WILLD”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 11, Nov. 2017, pp. 190-3, doi:10.22159/ajpcr.2017.v10i11.20299.
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