IN VITRO ANTIOXIDANT AND ANTIHYPERGLYCEMIC ACTIVITIES OF HYDROETHANOLIC LEAF EXTRACT OF ACACIA CATECHU (L.F) WILLD

Ramesh B; Jayabharathi V

doi:10.22159/ajpcr.2017.v10i11.20299

Authors

Ramesh B Department of Biochemistry, PSG College of Arts & Science, Coimbatore, Tamil Nadu, India. Email
Jayabharathi V Department of Biochemistry, PSG College of Arts & Science, Coimbatore, India.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i11.20299

Keywords:

Acacia catechu (Lf) Willd, 1, 1-diphenyl-1-picryl hydrazyl, Hydroxyl radical, Nitric oxide radical, Superoxide oxide radical, Nil

Abstract

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Â Objective: The aim of this study was to analyze the antioxidant and antihyperglycemic activities of hydroethanolic leaf extract of Acacia catechu (L.f) Willd.

Methods: The A. catechu (L.f) Willd. leaf extract was subjected to antioxidant activities such as 1,1-diphenyl-1-picryl hydrazyl (DPPH), hydroxyl radical (OHâˆ’), nitric oxide radical (NOâˆ’), and superoxide radical (SO) scavenging assays. Further, the leaf extract was subjected to antihyperglycemic activity by Î±-amylase inhibition assay.

Results: The free radical scavenging activity of the plant extract was found to increase with increase in concentration (20-100 Î¼g/ml) and the maximum activity was obtained at 100 Î¼g/ml, which was compared with the standard ascorbic acid. The percentage inhibition of free radicals was found to be 62.94Â±0.91%, 63.53Â±1.08%, 71.04Â±0.48%, and 51.50Â±0.86% for DPPH, OHâˆ’, NOâˆ’, and SOâˆ’, respectively. The IC50 for DPPH, OHâˆ’, NOâˆ’, and SO were recorded as 73.81Â±0.95, 69.03Â±0.58, 60.74Â±0.37, and 83.10Â±1.00 Î¼g/ml. The IC50 value of Î±-amylase inhibitory activity was found to be 71.17 Î¼g/ml.

Conclusion: The present investigations suggest that the A. catechu (L.f) Willd may serve as a potential source of natural antioxidant and antihyperglycemic agent that could have great importance in pharmaceutical preparations.

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