SCREENING OF TEN HERBAL EXTRACTS TO COMPARE AND INVESTIGATE EFFECT ON GLUCOSE LOWERING AND ANOREXIC ACTIVITY IN WISTAR RATS


Deepti Kaushalkumar Jani, Sunita Goswami

Abstract


Objective: Metabolic disorders are of growing concern with increasing quest for herbal medicines as treatment option. The current study is planned to screen ten herbal extracts, prepared from parts of chosen six herbal plants, to compare and investigate their antihyperglycemic and anorexic potential.

Methods: Qualitative phytochemical investigation was done as per standard procedures. Acute oral toxicity study performed as per OECD 420 guideline. Glucose Tolerance Test (method optimization) was performed to select appropriate glucose load to induce hyperglycemia. Antihyperglycemic activity was evaluated by Glucose Tolerance Test (single dose and multiple dose study) and anorexic potential was screened by food intake measurement using Wistar rats.

Results: All herbs were found safe in acute oral toxicity study. Glucose Tolerance Test method optimization study signaled use of 3 g/kg glucose load for induction of hyperglycemia in single and multiple dose studies. In single dose study, L. acutangula fruit extract and in multiple dose study, S. melongena, M. oleifera & R. communis extracts were found to produce significant glucose lowering effect as compared to glucose control group. Results of food intake measurement showed significant appetite suppression with extracts of W. somnifera, S. melongena, M. oleifera & R. communis.  

Conclusion: Present study throws light on the effectiveness of experimental herbal extracts as antihyperglycemic and anorexic agents. This also, helps in identifying potent indigenous herbs for further study. 


Keywords


Glucose tolerance test, Herbal medicine, Hyperglycemia.

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References


Pugliese A. The multiple origins of Type 1 diabetes. Diabet Med 2013;30(2):135-46.

Fotino N, Fotino C, Pileggi A. Re-engineering islet cell transplantation. Pharmacol Res 2015;98:76-85.

Stein SA, Lamos EM, Davis SN. A review of the efficacy and safety of oral antidiabetic drugs. Expert Opin Drug Saf 2013;12(2):153-75.

World Health Organization. Obesity and Overweight; 2016. Available from: http://www.who.int/mediacentre/factsheets/fs311/en.

Ruhm CJ. Understanding overeating and obesity. J Health Econ 2012;31(6):781-96.

Kang JG, Park CY. Anti-obesity drugs: A review about their effects and safety. Diabetes Metab J 2012;36:13-25.

Jerine PS, Evan PS. Global current trends in natural products for diabetes management: A review. Int J Pharm Pharm Sci 2016;8(4):20-8.

Beevi SS, Narasu ML, Gowda BB. Polyphenolics profile, antioxidant and radical scavenging activity of leaves and stem of Raphanus sativus L. Plant Foods Hum Nutr 2010;65(1):8-17.

Beevi SS, Mangamoori LN, Dhand V, Ramakrishna DS. Isothiocyanate profile and selective antibacterial activity of root, stem, and leaf extracts derived from Raphanus sativus L. Foodborne Pathog Dis 2009;6(1):129-36.

Pimple BP, Kadam PV, Patil MJ. Antidiabetic and antihyperlipidemic activity of Luffa acutangula fruit extracts in streptozotocin induced NIDDM rats. Asian J Pharm Clin Res 2011;4 Suppl 2:156-63.

Reddy BP, Mohan SV, Sarma PN. Antioxidant and antimicrobial activity of Luffa acutangula fruit extract based on total phenolic content as index. J Chem Pharm Sci 2010;3(4):218-23.

Nagarajaiah SB, Prakash J. Chemical composition and bioactive potential of dehydrated peels of Benincasa hispida, Luffa acutangula, and Sechium edule. J Herbs Spices Med Plants 2015;21:193-202.

Udayakumar R, Kasthurirengan S, Mariashibu TS, Rajesh M, Anbazhagan VR, Kim SC, et al. Hypoglycaemic and hypolipidaemic effects of Withania somnifera root and leaf extracts on alloxan-induced diabetic rats. Int J Mol Sci 2009;10(5):2367-82.

Alam N, Hossain M, Mottalib MA, Sulaiman SA, Gan SH, Khalil MI. Methanolic extracts of Withania somnifera leaves, fruits and roots possess antioxidant properties and antibacterial activities. BMC Complement Altern Med 2012;12:175.

Anwar F, Latif S, Ashraf M, Gilani AH. Moringa oleifera: A food plant with multiple medicinal uses. Phytother Res 2007;21(1):17-25.

Luqman S, Srivastava S, Kumar R, Maurya AK, Chanda D. Experimental assessment of Moringa oleifera Leaf and fruit for its antistress, antioxidant, and scavenging potential using in vitro and in vivo assays. Evid Based Complement Alternat Med 2012;2012:519084.

Guimarães PR, Galvão AM, Batista CM, Azevedo GS, Oliveira RD, Lamounier RP, et al. Eggplant (Solanum melongena) infusion has a modest and transitory effect on hypercholesterolemic subjects. Braz J Med Biol Res 2000;33(9):1027-36.

Satam NK, Parab LS, Bhoir SI. HPTLC finger print analysis and antioxidant activity of flavonoid fraction of Solanum melongena Linn fruit. Int J Pharm Pharm Sci 2013;5(3):734-40.

Ilavarasan R, Mallika M, Venkataraman S. Anti-inflammatory and free radical scavenging activity of Ricinus communis root extract. J Ethnopharmacol 2006;103(3):478-80.

Chattopadhyay RR. Possible mechanism of antihyperglycemic effect of Azadirachta indica leaf extract: Part V. J Ethnopharmacol 1999;67(3):373-6.

Trease GE, Evans WC. Pharmacognosy. 13th ed. London: Bailliere Tindall; 1989.

Harborne JB. Phytochemical Methods. London: Chapman and Hall Ltd.; 1973. p. 99-188.

Sofowara A. Medicinal Plants and Traditional Medicine in Africa. Ibadan, Nigeria: Spectrum Books Ltd.; 1993. p. 289.

OECD Test 420. Acute Oral Toxicity-Fixed dose Procedure. Vol. 4. OECD Guidelines for Testing of Chemicals; 2001. p. 1-4.

Elberry AA, Harraz FM, Ghareib SA, Gabr SA, Nagy AA, Abdel-Sattar E. Methanolic extract of Marrubium vulgare ameliorates hyperglycemia and dyslipidemia in streptozotocin-induced diabetic rats. Int J Diabetes Mellitus 2015;3(1):37-44.

Bjursell M, Egecioglu E, Gerdin AK, Svensson L, Oscarsson J, Morgan D, et al. Importance of melanin-concentrating hormone receptor for the acute effects of ghrelin. Biochem Biophys Res Commun 2005;326(4):759-65.

Khairunisa D, Pratiwi A, Setiawan AA, Dahlan K, Kartono A. Analysis of oral glucose tolerance test using modified oral minimal model to diagnose of Type 2 diabetes. Int J Pharm Pharm Sci 2015;7(8):313-6.

Reaven GM, Brand RJ, Chen YD, Mathur AK, Goldfine I. Insulin resistance and insulin secretion are determinants of oral glucose tolerance in normal individuals. Diabetes 1993;42(9):1324-32.

Song Y, Manson JE, Buring JE, Sesso HD, Liu S. Associations of dietary flavonoids with risk of Type 2 diabetes, and markers of insulin resistance and systemic inflammation in women: A prospective study and cross-sectional analysis. J Am Coll Nutr 2005;24(5):376-84.

Babu PV, Liu D, Gilbert ER. Recent advances in understanding the anti-diabetic actions of dietary flavonoids. J Nutr Biochem 2013;24(11):1777-89.

Agwaya MS, Vuzi PC, Nandutu AM. Hypoglycemic activity of aqueous root bark extract Zanthoxylum chalybeum in alloxan-induced diabetic rats. J Diabetes Res 2016;2016:8727590.

Vickers SP, Jackson HC, Cheetham SC. The utility of animal models to evaluate novel anti-obesity agents. Br J Pharmacol 2011;164(4):1248-62.




About this article

Title

SCREENING OF TEN HERBAL EXTRACTS TO COMPARE AND INVESTIGATE EFFECT ON GLUCOSE LOWERING AND ANOREXIC ACTIVITY IN WISTAR RATS

Topics

Pharmacy (Pharmacology)

Keywords

Glucose tolerance test, Herbal medicine, Hyperglycemia.

DOI

10.22159/ajpcr.2017.v10i12.20869

Date

01-12-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 12 December 2017 Page: 160-165

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

18 Views | 29 Downloads

Authors & Affiliations

Deepti Kaushalkumar Jani
1Department of Pharmacology, Babaria Institute of Pharmacy, Varnama, Vadodara, Gujarat, India. 2Department of Pharmacology, L.M. College of Pharmacy, Ahmadabad, Gujarat, India.
India

Sunita Goswami
1Department of Pharmacology, Babaria Institute of Pharmacy, Varnama, Vadodara, Gujarat, India. 2Department of Pharmacology, L.M. College of Pharmacy, Ahmadabad, Gujarat, India.
India


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