PHYTOCHEMICAL SCREENING AND ANTIDIABETIC ACTIVITY OF DIFFERENT LEAF EXTRACTS FROM LOTUS (NELUMBO NUCIFERA GAERTN.) IN STREPTOZOTOCIN INDUCED MICE

Authors

  • Ruth Mayana Rumanti Department of Biological Pharmacy, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia.
  • Marline Nainggolan Department of Biological Pharmacy, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia.
  • Urip Harahap Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i12.20888

Keywords:

Lotus, Diabetes mellitus, Antidiabetic, Streptozotocin, Mice

Abstract

 

 Objective: The present study is to evaluate the antidiabetic activity of various extract of Lotus (Nelumbo nucifera Gaertn.) leaf using hexane, ethyl acetate, and ethanol organic solvent.

Methods: Standard phytochemical screening method and streptozotocin induced diabetic mice.

Result: The phytochemical screening showed only ethyl acetate of lotus leaf extract contains the flavonoids. The ethyl acetate lotus leaf extract (dose level 150 mg/kg) showed a significant reduction of the blood glucose.

Conclusion: The ethyl acetate extract of lotus leaf (N. nucifera Gaertn.) had the hypoglycemic effect on diabetic induced mice.

Downloads

Download data is not yet available.

Author Biographies

Ruth Mayana Rumanti, Department of Biological Pharmacy, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia.

Department of Biological Pharmacy

Marline Nainggolan, Department of Biological Pharmacy, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia.

Department of Biological Pharmacy

Urip Harahap, Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia.

Department of Pharmacology

References

King H, Aubert RE, Herman WH. Global burden of diabetes, 1995-2025: Prevalence, numerical estimates, and projections. Diabetes Care 1998;21(9):1414-31.

Zhang Y, Wu L, Ma Z, Cheng J, Liu J. Anti-diabetic, anti-oxidant and anti-hyperlipidemic activities of flavonoids from corn silk on STZ-induced diabetic mice. Molecules 2015;21:E7.

Parthasarathy R, Illavarasan R, Karrunakaran CM. Antidabetic activity of Thespesia populnea bark and leaf extrct against streptozotocin induced diabetic rats. Int J Pharmtech Res 2009;1(4):1069-72.

WHO. Definition and Diagnosis of Diabetes Mellitus and Intermediate Hyperglycemia. Geneva: WHO; 2006. p. 1-50.

Sikarwar MS, Patil MB, Kokate CK, Sharma S, Bhat V. Antidiabetic activity of Nerium indicum Leaf extractin alloxan-induced diabetic rats. J Young Pharm 2009;1(4):330-5.

Rumanti RM. Characterization of Simplicia and Chromatographic Analysis of Active Extract Lotus (Nelumbo nucifera Gaertn) which can Reduce Blood Glucose in mice. Thesis, Faculty of Pharmacy, University of Sumatera Utara No. 1-104; 2017.

Lal VK, Gupta PP, Awanish P. Hypoglycemic effect of Kyllinga trceps in STZ induced diabetic rats. J Diabetes Metab 2012;3(6):1-3.

Acosta-Patiño JL, Jiménez-Balderas E, Juárez-Oropeza MA, Díaz-Zagoya JC. Hypoglycemic action of Cucurbita ficifolia on Type 2 diabetic patients with moderately high blood glucose levels. J Ethnopharmacol 2001;77(1):99-101.

Moro CF, Yonekura M, Kouzuma Y, Agrawal GK, Rakwal R. Lotus-a source of food and medicine: Current status and future perspective in context of the seed proteomics. Int J Life Sci 2013;7(1):1-5.

Sheikh SA. Ethno-medicinal uses and pharmacological activities of lotus. J Med Plants Stud 2014;2(6):42-6.

Liu CM, Kao CL, Wu HM, Li WJ, Huang CT, Li HT, et al. Antioxidant and anticancer aporphine alkaloids from the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena. Molecules 2014;19(11):17829-38.

Xiaotian C, Changyin W, Jianxin C, Gbago O, Yuanda S. Antibacterial activity of lotus leaves (Nelumbo nucifera) against food-borne pathogens. Am J Biochem Biotechnol 2015;11(1):11-6.

Ditjen POM. Farmakope Indonesia. 4th ed. Jakarta: Depkes RI; 1995. p. 896-8.

Depkes RI. Materia Medika. 6th ed. Jakarta: Ditjen POM; 1995. p. 297-307.

Farnsworth NR. Biologycal and phytochemical screening of plants. J Pharm Sci 1996;55(3):225-76.

Harbone JB. Metode Fitokimia. Vol. 2. Bandung: ITB; 1987. p. 49.

Bharathi TR, Prakash HS. Comparative evaluation of antidiabetic and antioxidant potency of different extracts obtained from Memecylon species. Int J Pharm Pharm Sci 2017;9(2):187-91.

Priyanka P, Singh R. A systematic review on Indian floral biodiversity as eminent reserves for alternative treatment strategy of diabetes mellitus. Int J Pharm Pharm Sci 2016;8(4):10-9.

Kar MK, Swain TR, Mishra SK. Antidiabetic activity of Clerodendrum serratum moon leaves in streptozotocin-induced diabetic rats. Asian J Pharm Clin Res 2014;7(5):260-3.

Mujic A, Grdovic N, Mujic I, Mihailovic M, Zivkovic J, Poznanovic G, et al. Anti-oxidative effects of phenolic extracts from chestnut leaves, catkins and spiny burs in streptozotocin-treated rat pancreatic β-cells. Food Chem 2011;125:841-9.

Published

01-12-2017

How to Cite

Rumanti, R. M., M. Nainggolan, and U. Harahap. “PHYTOCHEMICAL SCREENING AND ANTIDIABETIC ACTIVITY OF DIFFERENT LEAF EXTRACTS FROM LOTUS (NELUMBO NUCIFERA GAERTN.) IN STREPTOZOTOCIN INDUCED MICE”. Asian Journal of Pharmaceutical and Clinical Research, vol. 10, no. 12, Dec. 2017, pp. 190-2, doi:10.22159/ajpcr.2017.v10i12.20888.

Issue

Section

Original Article(s)