Yassir Haider Almawlah, Hadeel Alaa, Sanaa O Aljelawi


Objective: The increasing of antibiotic resistance of Pseudomonas aeruginosa causes serious worldwide infection problems. Hence, the objective of the study was to investigate the antibacterial activity of some plants against multidrug resistance P. aeruginosa isolated from burn-wound infections.

Methods: About 30 burn-wound swab samples taken from hospitalized patients in Al-Hillah Teaching Hospital, Babylon Province. P. aeruginosa isolates were isolated and identified depending on cultural, microbial, and biochemical characteristics. Then, the drug susceptibility was detected using different available antibiotics (8) to select multidrug resistance P. aeruginosa strains for using as test organisms. Three types of plants, including onion bulbs (Allium cepa), leaves of mint (Mentha asiatica), and outer peel of pomegranate (Punica granatum), were extracted by three types of solvent. The plant extracts were tested as antimicrobial substances.

Results: About 9 samples were found positive by causing bacterial infection who presented invasive burn-wound infection from both sex and average age of 9 to 45 years. P. aeruginosa was found to be the most common isolates, 10 samples out of 30 samples. The most of multidrug resistance P. aeruginosa was used as test organisms to investigate the antimicrobial activity of three types of plant extracts. The plant extract of P. granatum showed the highest antibacterial activity, followed by A. cepa, and finally, M. asiatica.

Conclusion: From the study, all three studied plants had antibacterial activity against multidrug-resistant P. aeruginosa isolated from burn wound. It is a recommendation that natural products can use as therapeutic agents will probably not elicit resistance in bacteria. More research must continue to isolate and purify the active components and applied in experimental animal models.



Multidrug resistance Pseudomonas aeruginosa, Allium cepa, Mentha asiatica, Punica granatum.

| PDF |


Armour AD, Shankowsky HA, Swanson T, Lee J, Tredget EE. He impact of nosocomially-acquired resistant Pseudomonas aeruginosa infection in a burn unit. J Trauma 2007;63(1):164-71.

Geyik MF, Aldemir M, Hosoglu S, Tacyildiz HI. Epidemiology of burn unit infections in children. Am J Infect Control 2003;31:342-6.

Kianbakht S, Jahaniani F. Evaluation of antibacterial activity of Tribulus terrestris L. growing in Iran. Iran J Pharm Ther 2003;2:22-4.

Eloff JN. Which extractant should be used for the screening and isolation of antimicrobial components from plants? J Ethnopharmacol 1998;60(1):1-8.

El-Taweil M. Assessment of antimicrobial activity of onion extract (Alliumcepa) on Staphylococcus aureus; In vitro study. Int Conf Chem Agric Med Sci 2014;29(30):16-7.

Hayyan I, Al-Taweil M. Antimicrobial effect of mint essential oils on some pathogenic bacteria. Int J Life Sci Res 2014;2(4):90-3.

Al-Zoreky NS. Antimicrobial activity of pomegranate (Punica granatum L.) fruit peels. Int J Food Microbiol 2009;134(3):244-8.

Forbes BA, Sahm DF, Weissfeld AS. Bailley and Scott Diagnostic Microbiology. 10th ed. London: Mosby; 1998.

Raja NS, Singh NN. Antimicrobial susceptibility pattern of clinical isolates of Pseudomonas aeruginosa in a tertiary care hospital. J Microbiol Immunol Infect 2007;40(1):45-9.

Okigbo RN, Mmeka EC. Antimicrobial effects of three tropical plant extracts on Staphylococcus aureus, Escherichia coli and Candida albicans. Afr J Tradit Complement Altern Med 2008;5:226-9.

Sama Fonkeng L, Mouokeu RS, Tume C, Njateng GS, Kamcthueng MO, Ndonkou NJ, et al. Anti - Staphylococcus aureus activity of methanol extracts of 12 plants used in Cameroonian folk medicine. BMC Res Notes 2015;8:710.

Irobi ON, Moo-Young M, Anderson WA, Daramola SO. Antimicrobial activity of bark extracts of Bridelia ferruginea (Euphorbiaceae). J Ethnopharmacol 1994;43:185-90.

Omran R. Production of antimicrobial and anticancer from featherkeratinolytic Nocardiopsis sp. 28ROR as a novel strain using feather meal medium. Int J Pharm Pharm Sci 2017;9(3):175-9.

Magaldi S, Mata-Essayag S, Hartung de Capriles C, Perez C, Colella MT, Olaizola C, et al. Well diffusion for antifungal susceptibility testing. Int J Infect Dis 2004;8:39-45.

Smânia EF, Valgas C, De Souza SM. Screening methods todetermine antibacterial activity of natural products. Braz J Microbiol 2007;38(2):369-80.

Mooney DP, Gamelli RL. Sepsis following thermal injury. Compr Ther 1989;15:22-9.

Radi RO, Rahman FM. Study of effects ethidium bromide, SDS and elevated temperature on stability of multiple antibiotic resistance plasmids of Pseudomonas aeruginosa. Iraqi J Biotech 2010;9(4):797-11 .

Omran R. The virulence factors of Pseudomonas aeruginosa isolated from eye infections. J Karbala Univ 2005;5:112-1.

Kehinde AO, Ademola SA, Okesola AO, Oluwatosin OM. Pattern of bacterial pathogens in burn wound infections in Ibadan, Nigeria. Ann Burns Fire Dis 2004;17:348-55.

Brown PD, Izundu A. Antibiotic resistance in clinical isolates of Pseudomonas aeruginosa in Jamica. Rev Panam Salud Publica 2004;16(2):125-30.

Negi PS, Jayaprakasha GK, Jena BS. Antioxidant and antimutagenic activities of pomegranate peel extracts. Food Chem 2003;80:393-7.

Hayrapetyan H, Hazeleger WC, Beumer RR. Inhibition of Listeria monocytogenes by pomegranate (Punica granatum) peel extract in meat pat´e at different temperatures. Food Control 2012;23:66-72.

Li Y, Guo C, Yang J, Wei J, Xu J, Cheng S. Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chem 2006;96(2):254-60.

Ahmad I, Beg AZ. Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. J Ethnopharmacol 2001;74(2):113-23.

Oskay M, Oskay D, Kalyoncu F. Activity of some plant extracts against multi-drug resistant human pathogens. Iran J Pharm Res 2009;8(4):293-300.

Nuamsetti T, Dechayuenyong P, Tantipaibulvut S. Antibacterial activity of pomegranate fruit peels and arils. Sci Asia 2012;38:319-22.

Voravuthikunchai S, Lortheeranuwat A, Jeeju W, Sririrak T, Phongpaichit S, Supawita T. Effective medicinal plants against enterohaemorrhagic Escherichia coli O157:H7. J Ethnopharmacol


Grover A, Bhandari B, Nishant R. Antimicrobial activity of medicinal plants - Azadirachta indica A. Juss, Allium cepa L. and Aloe vera L. Int J Pharm Tech Res 2011;3(2):1059-65.

Hendrich A. Flavonoid-membrane interactions: Possible consequences for biological effects of some polyphenolic compounds. Acta Pharmacol Sin 2006;27(1):27-40.

Ani V, Varadaj M, Naidu K. Antioxidant and antibacterial activities of polyphenolic compounds from bitter cumin (Cuminum rigrum L.). Eur Food Res Technol 2006;224(1):109-15.

Perez C, Pauli M, Bazerque P. An antibiotic assay by the agar-well diffusion method. Acta Biol Med Exp 1990;15:113-5.

Al-Marzook FA, Omran R. Cytotoxic activity of alkaloid extracts of different plants against breast cancer cell line. Asian J Pharm Clin Res 2017;10(7):168-71.

Omran R, Al-Taee ZM, Hashim HO, AL-Jassani MJ. Extraction of phenolic compounds as antioxidant from some plants and their cytotoxic activity against breast cancer cell line. Asian J Pharm Clin Res 2017;10(7):168-71.

Al-Marzook FA, Omran R. Cytotoxic activity of alkaloid extracts from three Iraqi plants against breast cancer cell line. Asian J Pharm Clin Res 2017;10(9):78-81.

Adebayo OL, Edwin BA. Solvent extract of different plants of Phyllanthus niruri show potent growth inhibition against six human pathogenic bacteria in vitro. Int J Pharm Pharm Sci 2014;6(7):272-7.

Bupesh C, Amutha S, Nandagopal A, Ganeshkumar P, Sureshkumar K, Murali S. Antibacterial activity of Mentha piperita L. (Peppermint) from leaf extracts-a medicinal plant. Acta Agric Slov 2007;89:73-9.

Sartoratto A, Machado AL, Delarmelina C, Figueira GM, Duarte MC, Rehder VL. Composition and antimicrobial activity of essential oils from aromatic plants used in Brazil. Braz J Microbiol 2004;34(4):1517-20.

About this article




Multidrug resistance Pseudomonas aeruginosa, Allium cepa, Mentha asiatica, Punica granatum.





Additional Links

Manuscript Submission


Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 12 December 2017 Page: 193-197

Print ISSN


Online ISSN


Authors & Affiliations

Yassir Haider Almawlah
DNA Research Center, University of Babylon, Babel, Iraq.

Hadeel Alaa
DNA Research Center, University of Babylon, Babel, Iraq.

Sanaa O Aljelawi
DNA Research Center, University of Babylon, Babel, Iraq.

Article Tools

Email this article (Login required)
Email the author (Login required)


  • There are currently no refbacks.