ANTIMICROBIAL ANALYSIS OF DIFFERENT PARTS EXTRACT IN DIFFERENT SOLVENT SYSTEM OF A WASTE WEED - CALOTROPIS PROCERA.


Dattatreya Kar, Pratap Keshari Pattanaik, Bibhudutta Pattnaik, Ananya Kuanar

Abstract


 Objective: In the current study, we have focused on the major secondary metabolite containing parts such as flower, leaf, and root for phytochemical extraction with three different solvent systems to make a comparative study against three virulent bacteria species which are capable of intestinal infection, pneumonia, skin infections, and food poisoning.

Methods: Antimicrobial activity of ethanol, methanol, and chloroform extracts from bark, leaves and roots of Calotropis procera, was examined against three virulent bacteria species: Escherichia coli, Staphylococcus aureus, and Bacillus subtilis using disc diffusion method.

Results: The ethanol extract of leaf showed significant activity against S. aureus with a zone of inhibition ranging from 14 to 20 mm for S. aureus. The ethanol extract of flower was effective against E. coli with maximum 18 mm. Ethanol extract of root showed significant activity against S. aureus. Methanol extract of leaves showed moderate activity against S. aureus with a zone of inhibition ranging from 14 to 20 mm. Methanol extract of root showed significant activity against S. aureus with a zone of inhibition ranging from 12 to 22 mm. Methanol extract of flowers showed activity against E. coli with a zone of inhibition ranging from 11 to 20 mm. The chloroform extract of leaves showed significant activity against S. aureus. Chloroform extract of flower showed activity with zone of inhibition ranging from 11 to 17 mm for S. aureus chloroform extract of root showed activity against E. coli with zone of inhibition ranging from 9 to 17 mm.

Conclusion: From the above study, it can be concluded that the activity of the plant extract may be due to the secondary metabolites or broad-spectrum antibiotic compounds present in it.


Keywords


Calotropis procera, Antimicrobial, Weeds.

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About this article

Title

ANTIMICROBIAL ANALYSIS OF DIFFERENT PARTS EXTRACT IN DIFFERENT SOLVENT SYSTEM OF A WASTE WEED - CALOTROPIS PROCERA.

Keywords

Calotropis procera, Antimicrobial, Weeds.

DOI

10.22159/ajpcr.2018.v11i2.21081

Date

01-02-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Issue 2 February 2018 Page: 227-230

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

46 Views | 35 Downloads

Authors & Affiliations

Dattatreya Kar
Centre of Biotechnology, Siksha O Anusandhan University, Kalinga Nagar, Ghatikia,Bhubaneswar - 751 003, Odisha, India.
India

Pratap Keshari Pattanaik
Department of Microbiology, College of Basic Science and Humanities, Orissa University of Agriculture and Technology, Surya Nagar, Bhubaneswar - 751 003, Odisha, India
India

Bibhudutta Pattnaik
Centre of Biotechnology, Siksha O Anusandhan University, Kalinga Nagar, Ghatikia,Bhubaneswar - 751 003, Odisha, India
India

Ananya Kuanar
Centre of Biotechnology, Siksha O Anusandhan University, Kalinga Nagar, Ghatikia,Bhubaneswar - 751 003, Odisha, India
India


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