VANILLIN EXTRACTED FROM PROSO MILLET AND BARNYARD MILLET INDUCE APOPTOSIS IN HT-29 AND MCF-7 CELL LINE THROUGH MITOCHONDRIA MEDIATED PATHWAY

Deepa Priya Ramadoss; Nageswaran Sivalingam

doi:10.22159/ajpcr.2017.v10i12.21088

Authors

Deepa Priya Ramadoss Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Kan
Nageswaran Sivalingam Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Kan http://orcid.org/0000-0001-8998-8622

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i12.21088

Keywords:

Proso millet, Barnyard millet, Vanillin, HT-29 cells, MCF-7 cells, Cytochrome c, Bax, Caspase-9

Abstract

Objective: The main aim of the study was to investigate the bioactive compound vanillin extracted from proso millet (compound 1), and barnyard millet (compound 2) induces apoptotic cell death and whether it is mediated through mitochondrial pathway in HT-29 and MCF-7 cell line.

Methods: The cells were treated with 250 Î¼g/ml and 1000 Î¼g/ml concentration of extracted vanillin for 48 hrs. Cytochrome c release and expression level of pro-apoptotic protein Bax and caspase-9 were detected by western blot analysis.

Results: The results reveal that extracted compounds increased the release of cytochrome c and upregulating the expression of Bax and caspase-9 as concentration increases in a dose-dependent manner.

Conclusion: The study suggests that the vanillin compound extracted from these millets induces apoptotic cell death through a mitochondria-dependent pathway.

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References

Jemal A, Siegel R, Xu J, Ward E. Cancer statistics, 2010. CA Cancer J Clin 2010;60(5):277-300.

El-Hafeez AA, Rakha OM. Paederus alfieri extract induces apoptosis in human myeloid leukemia K562 Cells. Asian J Pharm Clin Res 2017;10:70-3.

Sumalatha D, Nithya TG. In vitro anti-oxidant and anticancer activity of Murraya koenigii against human colon cancer HT-29 cell lines. Asian J Pharm Clin Res 2014;7:83-6.

Boyle P, Levin B. World cancer report 2008. Cancer Control 2008;199:12-497.

Moongkarndi P, Kosem N, Kaslungka S, Luanratana O, Pongpan N, Neungton N. Antiproliferation, antioxidation and induction of apoptosis by Garcinia mangostana (mangosteen) on SKBR3 human breast cancer cell line. J Ethnopharmacol 2004;90(1):161-6.

Cragg GM, Newman DJ. Plants as a source of anti-cancer agents. J Ethnopharmacol 2005;100(1-2):72-9.

Harvey AL. Natural products as a screening resource. Curr Opin Chem Biol 2007;11(5):480-4.

Harvey A. Strategies for discovering drugs from previously unexplored natural products. Drug Discov Today 2000;5(7):294-300.

Babak E, Yari KY, Ali AM, Rahmani S, Rahimi M, Amanlou M. Anti-cariogenic properties of malvidin-3,5-diglucoside isolated from Alcea longipedicellata against oral bacteria. Int J Pharmacol 2007;3:468-74.

Newman DJ, Cragg GM. Natural products as sources of new drugs over the last 25 years. J Nat Prod 2007;70(3):461-77.

Neerugatti DB, Battu GR, Bandla R. Cytotoxicity activity of some Indian medicinal plants. Int J Curr Pharm Res 2016;8:86-8.

Rajamanickam S, Agarwal R. Natural products and colon cancer: Current status and future prospects. Drug Dev Res 2008;69(7):460-71.

Ramasamy S, Abdul Wahab N, Zainal Abidin N, Manickam S, Zakaria Z. Growth inhibition of human gynecologic and colon cancer cells by Phyllanthus watsonii through apoptosis induction. PLoS One 2012;7(4):e34793.

Sepehr S, Sepehri H, Khodagholi F, Delphi L. Apple pectin (AP) induced apoptosis via nitric oxide (NO) in human prostate cancer cells du145. Int J Pharm Pharm Sci 2015;7:281-5.

Yang HL, Chen CS, Chang WH, Lu FJ, Lai YC, Chen CC, et al. Growth inhibition and induction of apoptosis in MCF-7 breast cancer cells by Antrodia camphorata. Cancer Lett 2006;231(2):215-27.

Regina AO, Asante IK, Safo DO, Tuffour I, Ofori-Attah E, Uto T, et al. Cytotoxic effects of Albizia zygia (DC) JF Macbr, a Ghanaian medicinal plant, against human T-lymphoblast-like leukemia, prostate and breast cancer cell lines. Int J Pharm Pharm Sci 2016;8:392-6.

Cheng WY, Hsiang CY, Bau DT, Chen JC, Shen WS, Li CC, et al. Microarray analysis of vanillin-regulated gene expression profile in human hepatocarcinoma cells. Pharmacol Res 2007;56(6):474-82.

Ho K, Yazan LS, Ismail N, Ismail M. Apoptosis and cell cycle arrest of human colorectal cancer cell line HT-29 induced by vanillin. Cancer Epidemiol 2009;33(2):155-60.

Bezerra DP, Soares AK, de Sousa DP. Overview of the role of vanillin on redox status and cancer development. Oxid Med Cell Longev 2016;2016:9734816.

Pedroso LS, FÃ¡vero GM, de Camargo LE, Mainardes RM, Khalil NM. Effect of the o-methyl catechols apocynin, curcumin and vanillin on the cytotoxicity activity of tamoxifen. J Enzyme Inhib Med Chem 2013;28(4):734-40.

Tai A, Sawano T, Yazama F, Ito H. Evaluation of antioxidant activity of vanillin by using multiple antioxidant assays. Biochim Biophys Acta 2011;1810(2):170-7.

Lirdprapamongkol K, Sakurai H, Kawasaki N, Choo MK, Saitoh Y, Aozuka Y, et al. Vanillin suppresses in vitro invasion and in vivo metastasis of mouse breast cancer cells. Eur J Pharm Sci 2005;25(1):57-65.

Harlow ED, Lane D. Using Antibodies: Cold Spring Harbor. New York: Cold Spring Harbor Laboratory Press; 1999.

Moss DW, Henderson AR. Determination of lactate dehydrogenase activity by measurement of NADH consumption. In: Carl AB, Edward RA, editors. Tietz Text Book of Clinical Chemistry. 2nd ed. Philadelphia, PA: W.B. Saunders Company; 1994. p. 816-8.

Wang JB, Qi LL, Zheng SD, Wu TX. Curcumin induces apoptosis through the mitochondria-mediated apoptotic pathway in HT-29 cells. J Zhejiang Univ Sci B 2009;10(2):93-102.

Lay MM, Karsani SA, Malek SN. 1-(2,6-dihydroxy-4-methoxyphenyl)-2-(4-hydroxyphenyl) ethanone-induced cell cycle arrest in G1/G0 in HT-29 cells human colon adenocarcinoma cells. Int J Mol Sci 2014;15(1):468-83.

Reed JC, Pellecchia M. Apoptosis-based therapies for hematologic malignancies. Blood 2005;106(2):408-18.

Hongme Z. Extrinsic and intrinsic apoptosis signal pathway review scientist. Apoptosis and medicine. Intech Open 2012;2:3-22.

Deckwerth TL, Elliott JL, Knudson CM, Johnson EM Jr, Snider WD, Korsmeyer SJ. BAX is required for neuronal death after trophic factor deprivation and during development. Neuron 1996;17(3):401-11.

Martinou I, Desagher S, Eskes R, Antonsson B, AndrÃ© E, Fakan S, et al. The release of cytochrome c from mitochondria during apoptosis of NGF-deprived sympathetic neurons is a reversible event. J Cell Biol 1999;144(5):883-9.

Finucane DM, Bossy-Wetzel E, Waterhouse NJ, Cotter TG, Green DR. Bax-induced caspase activation and apoptosis via cytochrome c release from mitochondria is inhibitable by Bcl-xL. J Biol Chem 1999;274(4):2225-33.

Daâ€™i M, Anti S, Edi M, Umar AJ, Masashi K. Apoptosis induction effect of curcumin and its analogs pentagamavunon-0 and pentagamavunon-1 on cancer cell lines. Asian J Pharm Clin Res 2017;10:5-8.

Opdyke DL. Monographs on fragrance raw materials: Vanillin. Food Cosmet Toxicol 1977;15:633-8.