IN VITRO FREE RADICAL SCAVENGING ACTIVITY AND IN VIVO ANTICATARACT POTENTIAL OF HEMIDESMUS INDICUS ROOTS IN STREPTOZOTOCIN INDUCED DIABETIC RATS


Prabhakar Tirumani, Rajashekhar Av

Abstract


Objective: Hemidesmus indicus R. Br. (HI) (Indian sarsaparilla) is traditionally used in Indian medicine for human therapy. The present study was aimed to evaluate the antioxidant activity of HI root extracts, and its effects on delaying (or) management of cataract progression in streptozoticin (STZ) induced diabetic rats.

Methods: The plant roots were extracted in different solvents, estimated total phenolic content, total flavonoid content and conducted free radical scavenging potential through 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2,2 azinobis (3-ethyl benzothiazoline-6-sulphonic acid diammonium salt (ABTS) NO, OH and reducing power assays. Further, the protective effect was evaluated in STZ induced diabetic rats through administered of ethyl acetate extract of HI (200 and 400 mg/kg body weight) orally for 8 weeks to study its effect on oxidative stress and diabetic cataract progression.

Results: In primary results demonstrated that the ethyl acetate extract has been showed considerable free radical scavenging activity and significantly gain body weight, gradual decreasing blood glucose levels and delay cataract progression has been observed in HI treated rats. Furthermore, we observed significantly decreased oxidative stress markers, and restoration of the antioxidant system in HI treated rats. Lens sections from experimental animal again evidenced to conform the ameliorative effect of HI on diabetic cataract.

Conclusion: This obtained result’s confirmed that the protective effect of HI is attributed to the antioxidant activities of plant roots and its active principles.


Keywords


Hemidesmus indicus, Free radicals, Antioxidant activity, Streptozotocin, Diabetic cataract

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References


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About this article

Title

IN VITRO FREE RADICAL SCAVENGING ACTIVITY AND IN VIVO ANTICATARACT POTENTIAL OF HEMIDESMUS INDICUS ROOTS IN STREPTOZOTOCIN INDUCED DIABETIC RATS

Topics

Dept. of Zoology, Osmania University

Keywords

Hemidesmus indicus, Free radicals, Antioxidant activity, Streptozotocin, Diabetic cataract

DOI

10.22159/ajpcr.2017.v10i12.21167

Date

01-12-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 12 December 2017 Page: 129-136

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

22 Views | 46 Downloads

Authors & Affiliations

Prabhakar Tirumani
Department of Zoology, Physiology Division, Osmania University, Hyderabad, Telangana, India.
India

Rajashekhar Av
Department of Zoology, Physiology Division, Osmania University, Hyderabad, Telangana, India.
India


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