EVALUATION OF THE ANTIMALARIAL POTENTIAL OF STREPTOMYCES SP.

Authors

  • Sweetline C Department of Microbiology, Karpagam Academy of Higher Education, Coimbatore - 641 021, Tamil Nadu, India.
  • Usha R Department of Microbiology, Karpagam Academy of Higher Education, Coimbatore - 641 021, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i2.21230

Keywords:

Actinomyces, Sequence, Antiplasmodial, Parasitemia, Malaria

Abstract

Objective: Isolate, screen and identify Streptomyces sp. from mangrove soil from pichavaram, Tamil Nadu, India, and study the molecular identification of selected Streptomyces sp. and check the antimalarial activity for the purified compound.

Methods: The16SrRNA secondary structure and the restriction sites of KMA08 were predicted using Genbank online software, respectively. Antiplasmodial activity of the 80% Ethyl acetate extract of Streptomyces sp. against chloroquine-sensitive Plasmodium berghei in mice using the 4 days suppression test was conducted. A total of 30 mice assigned to 5 groups of 6 animals each were infected with chloroquine-sensitive (P. berghei) intraperitoneally. The Ethyl acetate extract (10, 20, and 30 ml/kg), standard drug (chloroquine, 10 mg/kg) and distilled water were administered orally daily for the treatment period. Percent Parasitemia was determined on the 5th day from Giemsa stained smears obtained from tail vein and percent parasitemia suppression was calculated. Daily measurement of rectal temperature was also taken while body weight and packed cell volume were recorded on day 0 and 5.

Results: Results showed the extract produced a dose-dependent reduction in parasite density compared to the control group. Percept parasitemia calculation revealed 21.3, 65.3, and 80.5% inhibition at 10, 20, and 30 ml/kg of the extract, respectively.

Conclusion: The study revealed the present work indicated that Streptomyces sp. has as promising antiplasmodial activity against chloroquine sensitive P. berghei in a dose-dependent. As part of the drug discovery process, these promising finding may contribute to the medicinal and pharmaceutical field for malarial treatment.

References

Guadalupe E, Garcia L, Rodriguez JB. Current status and progress made in malaria chemotherapy. Curr Med Chem 2007;14:289-14.

Sahu NK, Sahu S, Kohli DV. Novel molecular target for antimalarial drug development. Chem Biol Drug Design 2008;71:287-97.

Basco LK, Mitaku S, Skaltsounis AL, Ravelomantosa N, Tillequin F, Koch M, et al. In vitro activities of furoquinoline and acridone alkaloids against P. falciparum. Antimicrob Agents Chemother 1994;38:1169-71.

Ibezim EC, Odo U. Current trends in malaria chemotherapy. Afr J Biotechnol 2008;7:349-56.

Ridley RG. Medical need, scientific opportunity and the drive for antimalarial drugs. Nature 2002;415:686-93.

World Economic Forum. Guidelines for Employer-Based Malaria Control Programmes. Massachusetts, USA: Global Health Initiatives and Harvard School of Public Health; 2006.

Sweetline C, Usha R, Dhanabalan R, Palaniswamy M. In vivo antimalarial activity of Streptomyces sp against Plasmodium berghei in mice model. Int J Curr Res 2014;6:7617-20.

Min Chung, Seo S, Kang E, Park O, Moon H. Anti-malarial activity of 6-(8’Z-pentadecenyl)-salicylic acid from Viola websteri in mice. Malar J 2007;8:151.

Waksman SA, Woodruff HB. Bacteriostatic and bacteriocidal substances produced by soil actinomycetes. Proc Soc Exp Biol Med 1940;45:609-14.

Pavani M, Girijasankar G, Mallika K, Sagar GV. Purification and molecular weight determination of Keratinase isolated from Streptomyces malaysiensis. Int J Pharm Pharm Sci 2017;9:154-8.

Cooks AE, Meyers PR. Rapid identification of filamentous actinomycetes to the genus level using genusspecific 16S rRNA gene restriction fragment patterns. Int J Syst Evol Microbiol 2003;53:1907-15.

Coombs JT, Franco CM. Isolation and identification of actinobacteria from surface sterilized wheat roots. Appl Environ Microbiol 2003;69:5603-8.

Eltayeb IM, Hami AS. Phytochemical screening and antimicrobial activity of Thuja occidentalis seeds extracts against the isolated common skin infecting microorganisms. Int J Pharm Pharm Sci 2017;9:20-4.

Peters W, Robinson B. The chemotherapy of rodent malarial. Studies on puronaridine and other manich base antimalarials. Ann Trop Med Parasitol 1992;86:455-65.

WHO. Control and Surveillance of African Trypanosomosis. Switzerland, Geneva: World Health Organization Technical Report Series No. 881; 1998.

David A, Fidock M, Philip J, Rosenthal L, Croft L, Reto B, et al. Antimalarial drug discovery: Efficacy models for compound screening. Protocols for antimalarial efficacy testing in vivo. Nat Rev 2004;3:28.

Peters W, Robinson B. Experimental models in antimicrobial chemotherapy. In Handbook of Animal Models of Infection. London: Elsevier Ltd.; 1999. p. 757-73.

Waako PJ, Gumede B, Smith P, Folb PI. The in vitro and in vivo antimalarial activity of Cardiospermum halicacabum L. and Momordica foetida schumch. Et thonn. J Ethnopharmacol 2005;99:137-43.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.

Lambros C, Vanderberg JP. Synchronization of Plasmodium falciparum erythrocytic stages in culture. J Parasitol 1979;65:418-20.

Abebe D, Debella A, Urga K. Medicinal plants and other useful plants of Ethiopia. ENHRI. Nairobi: Camerapix Publishers International; 2003. p. 299-301.

Geyid A, Abebe D, Debella A, Makonnen Z, Aberra F, Teka F, et al. Screening of some medicinal plants of Ethiopia for their antimicrobial properties and chemicals profiles. J Ethnopharmacol 2005;97:421-7.

Yineger H, Kelbessa E, Bekele T, Lulekal E. Ethnoveterinary medicinal plants at bale mountains national park, Ethiopia. J Ethnopharmacol 2007;112:55-70.

Vasant RA, Narasimhacharya AV. Antihyperglycemic and anti-hyperlipidemic effects of Mangifera indica L. In fluoride induced toxicity. Phamacol Online 2011;3:265-74.

Dikasso D, Makonnen E, Debella A, Abebe D, Urga K, Makonnen W, et al. In vivo antimalarial activity of hydroalcoholic extracts from Asparagus africanus Lam. in mice infected with Plasmodium berghei. Ethiop J Health Dev 2006;20:112-8.

Saxena S, Pant N, Jain DC, Bhakuni RS. Antimalarial agents from plant sources. Curr Sci 2003;85:1314-29.

Ramazani A, Zakeri S, Sardari S, Khodakarim N, Djadid N. In vitro and in vivo antimalarial activity of Boerhavia elegans and Solanum surattense. Malar J 2010;9:124.

Perlmann P, Troye-Blomberg M. Malaria Immunology. Stockholm, Sweden: Karger Publisher; 2007.

Taylor PJ, Hurd H. The influence of host haematocrit on the blood feeding success of Anopheles stephensi: Implications for enhanced malaria transmission. Parasitology 2001;122:491-6.

Chinchilla M, Guerrero OM, Abarca G, Barrios M, Castro O. An in vivo model to study the anti-malaric capacity of plant extracts. Rev Biol Trop 1998;46:35-9.

Jigam A, Akanya H, Bukar E, Obadoyi E. Antiplasmodial, analgesic and anti-inflammatory effects of Crude Guiera senegalensis Gmel Combretaceae Leaf extracts in mice infected with Plasmodium berghei. J Pharm Phytother 2011;3:150-4.

Hansen B, Pappas P. Effect of P. Berghei on the metabolic rate of mice. Ohio J Sci 1977;77:189-91.

Handayani S , Chiu D. T, Tjitra E . High deformability of Plasmodium vivax-infected red blood cells under microfluidic conditions. Journal of Infectious Diseases 2009 ; vol. 199, no. 3, pp. 445–450.

Rivo Y.B, Alkarimah A, Ramadhani N.N, Cahyono A.W, Laksmi D.A, Winarsih S, Fitri L.E. Metabolite extract of Streptomyces hygroscopicus Hygroscopicus inhibit the growth of Plasmodium berghei through inhibition of ubiquitin – proteasome system. Trop Biomed 2013; 30(2): 91–300.

Kondrashin AV , Rooney W. Overview: epidemiology of malaria and its control in countries of the WHO South-East Asia region. Out heast Asian J Trop Med & Public Health 1992; 23: 13-22.

Published

2018-02-01

How to Cite

C, S., and U. R. “EVALUATION OF THE ANTIMALARIAL POTENTIAL OF STREPTOMYCES SP.”. Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 2, Feb. 2018, pp. 354-8, doi:10.22159/ajpcr.2018.v11i2.21230.

Issue

Section

Original Article(s)