IN VITRO ANTIOXIDANT POTENTIAL AND HEPATOPROTECTIVE ACTIVITY OF TAXUS WALLICHIANA
Objectives: Taxus wallichiana, the member of family Taxaceae is used for the cure of various ailments by a large ethnic population native to the forests in Kashmir valley. The aim of this study is to evaluate antioxidant potential and hepatoprotective action of T. wallichiana as claimed by the ethnic population segment.
Methods: Antioxidant potential of different T. wallichiana extracts was evaluated by estimating the total phenolics, 1,1-diphenyl -2-picrylhydrazyl (DPPH), reducing power, microsomal lipid peroxidation (LPO), and hydroxyl radical scavenging activity through standard protocols. Hepatoprotective activity of T. wallichiana was determined by evaluation of liver marker enzyme (aspartate transaminase, alanine transaminase, and alkaline phosphatase) levels using standard protocols and by histological analysis of rat liver tissues of different animal groups against CCl4 induced liver damage.
Results: Phenolic content expressed as gallic acid equivalents (GAE/g) was found maximum in case of ethanolic extract (153.34 mg GAE/g), followed by extracts of methanol (149.24 mg GAE/g), hexane (134 mg GAE/g), ethyl acetate (128.9 mg GAE/g), chloroform (121.37 mg GAE/g), and aqueous (113.00 mg GAE/g). At concentration of 700 Î¼g/ml, DPPH radical scavenging activity of methanol extract was 88.29% with IC50 (212.00 Î¼g/ml), aqueous (82.47%) with IC50 (258.29 Î¼g/ml), and ethyl acetate (79.57%) with IC50 (301.80%Î¼g/ml). The reducing power of the extracts increased in a concentration dependent manner. At concentration of 700 Î¼g/ml, 79%, 68%, and 56% inhibition was observed with extracts of methanol, aqueous, and ethyl acetate on microsomal LPO with IC50 values of 126.09, 168.83, and151.96 Î¼g/ml, respectively. Superoxide radical scavenging activity of T. wallichiana extracts increased in a dose-dependent manner with IC50 values 170.30 Î¼g/ml (methanol), 257.00 Î¼g/ml (aqueous), and 297.55 Î¼g/ml ethyl acetate extract. T. wallichiana extracts exhibited antioxidant effects on calf thymus DNA damage. The results further depict the hepatoprotective action of T. wallichiana extract at doses of 100 and 300 mg/kg and were comparable with that of standard treatment comprising 50 mg/kg-day of Vitamin C, a known hepatoprotective agent. The data were substantiated with histopathological studies of rat liver sections.
Conclusion: These results comprehensively depict that T. wallichiana extracts are endowed with the free radical sequestering potential and be employed as potential antioxidant and hepatoprotective arsenal against many oxidative stress linked diseases.Â
2. Nisar M, Khan I, Simjee SU, Gilani AH, Perveen H. Anticonvulsant, analgesic and antipyretic activities of Taxus wallichiana Zucc. J Ethnopharmacol 2008;116:490-4.
3. Purohit A, Maikhuri RK, Rao KS, Nautiyal S. Impact of bark removal on survival of Taxus baccata L. (Himalayan yew) in Nanda Devi biosphere reserve, Garwhal Himalaya, India. Curr Sci 2001;81:586-90.
4. Khan I, Nisar M, Shah MR, Shah H, Gilani SN, Gul F, et al. Anti-inflammatory activities of Taxus abietane A isolated from Taxus wallichiana Zucc. Fitoterapia 2011;82:1003-7.
5. Qayum M, Nisar M, Shah MR, Adhikari A, Kaleem WA, Khan I, et al. Analgesic and antiinflammatory activities of taxoids from Taxus wallichiana Zucc. Phytother Res 2012;26:552-6.
6. Nisar M, Khan I, Ahmad B, Ali I, Ahmad W, Choudhary MI. Antifungal and antibacterial activities of Taxus wallichiana Zucc. J Enzyme Inhib Med Chem 2008;23:256-60.
7. Chattopadhyay SK, Pal A, Maulik PR, Kaur T, Garg A, Khanuja SP. Taxoid from the needles of the himalayan yew Taxus wallichiana with cytotoxic and immunomodulatory activities. Bioorg Med Chem Lett 2006;16:2446-49.
8. Gonzalez J. Medicinal plants in Colombia. J Ethnopharmacol 1980;2:43-7.
9. Sies H. Role of reactive oxygen species in biological processes. Wiener Klin Wochenschr 1991;69:965-8.
10. Halliwell B. Role of free radicals in the neurodegenerative diseases: Therapeutic implications for antioxidant treatment. Drugs Aging 2001;18:685-716.
11. Aviram M. Review of human studies on oxidative damage and antioxidant protection related to cardiovascular diseases. Free Radic Res 2000;33:S85-97.
12. Klaunig JE, Kamendulis LM. The role of oxidative stress in carcinogenesis. Annu Rev Pharmacol Toxicol 2004;44:239-67.
13. Stocker R, Keaney JF. Role of oxidative modifications in atherosclerosis. Physiol Rev 2004;84:1381-478.
14. Chandan BK, Saxena AK, Shukla S, Sharma N, Gupta DK, Singh K. Hepatoprotective activity of wood Fordia fruticosa Kurz flowers against carbon tetrachloride induced hepatotoxicity. J Ethnopharmacol 2008;119:218-24.
15. Feijoo M, TÃºnez I, Ruiz A, Tasset I, Munoz E, Collantes E. Oxidative stress biomarkers as indicator of chronic inflammatory joint diseases stage. Reumatol Clin 2010;6:91-4.
16. Basu S. Carbon Tetrachloride-Induced Hepatotoxicity: A Classic Model of Lipid Peroxidation and Oxidative Stress. In: Basu S, Wiklund L, editors. Studies on Experimental Models. Totowa: Humana Press; 2011.
17. Robins V, Kumar SL, Mitchell F. The Liver, Gallbladder, and Biliary Tract: Basic Pathology. 8th ed. Philadelphia, PA: W.B. Saunder Co.; 2007.
18. Masuda Y. Learning toxicology from carbon tetrachloride induced hepatotoxicity. Yakugaku Zasshi 2006;126:885-99.
19. Taniguchi M, Takeuchi T, Nakatsuka R, Watanabe T, Sato K. Molecular process in acute liver injury and regeneration induced by carbon tetrachloride. Life Sci 2004;75:1539-49.
20. McDonald S, Prenzler PD, Antolovich M, Robards K. Phenolic content and antioxidant activity of olive extracts. Food Chem 2001;73:73-84.
21. Kim DO, Lee LW, Lee HJ, Lee CY. Vitamin C equivalent antioxidant capacity (VCEAC) of phenolics phytochemicals. J Agric Food Chem 2002;50:3713-7.
22. Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Japan J Nutr 1986;44:307-315.
23. Halliwell B, Gutteridge JM, Aruoma OI. The deoxyribose method: a simple test-tubeâ€ assay for determination of rate constants for reactions of hydroxyl radicals. Anal Biochem 1987;165:215-9.
24. McCord JM, Fridovich I. Superoxide dismutase: An enzymic function for erythrocuprein (hemocuprein). J Biol Chem 1969;244:6049-55.
25. Chang ST, WU JH, Wang SY, Kang PL, Yang NS, Shyur LF. Antioxidant activities of extracts from Acacia confuse bark and heartwood. J Agric Food Chem 2001;49:3420-24.
26. Patrick-Iwuanyanwu KC, Amadi U, Charles IA, Ayalogu EO. Evaluation of acute and subchronic oral toxicity study of baker cleansers bitters-a polyherbal drug on experimental rats. EXCLI 2012;11:632-40.
27. David LW, Vincent M. Biochemistry in Clinical Practice. New York: Worth Publishers; Test Indicating Hepatocellular Damage; 1985.
28. Gajalakshmi S, Vijayalakshmi S, Rajeswari DV. Phytochemical and pharmacological properties of Annona muricata: A review. Int J Pharm Pharm Sci 2012;4:3-6.
29. Umamaheswari M, Chatterjee TK. In vitro antioxidant activities of the fractions of Coccinia grandis L leaf extract. Afr J Trad Compl Alt Med 2008;5:61-73.
30. Macwan CP, Patel MA. Antioxidant potential of dried root powder of Capparis zeylanica linn. Int J Pharm Pharm Sci 2010;2:58-60.
31. Beknal AK, Korwar PG, Halkai MA, Kulkarni U, Patil BS, Soodam SR. Phytochemical investigation and antioxidant activity study of Drynaria quercifolia linn rhizome. Int J Curr Pharm Res 2010;2:36-9.
32. Rice-Evans CA, Miller NJ, Bollwell PG, Bramley PM, Pridham JB. The relative antioxidant activities of plant derived polyphenolic flavonoids. Free Radic Res 1995;22:375-83.
33. Siddhuraju P, Mohan PS, Becker K. Studies on the antioxidant activity of Indian Laburnum (Cassia fistula L.): A preliminary assessment of crude extracts from stem bark, leaves, flowers and fruit pulp. J Agric Food Chem 2002;79:61-7.
34. Jayanthi P, Lalitha P. Reducing power of the solvent extracts of Eichhornia crassipes (mart.) solms. Int J Pharm Pharm Sci 2011;3:126 8.
35. Ferreira IC, Baptista P, Vilas-Boas M, Barros L. Free radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: Individual cap and stripe activity. Food Chem 2007;100:1511 6.
36. Irshad MD, Zafaryab MD, Rizvi MM. Comparative analysis of the antioxidant activity of Cassia fistula extracts. Int J Med Chem 2012;2:1 6.
37. Pulido R, Bravo L, Saura-Calixto F. Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. J Agric Food Chem 2000;48:3396-402.
38. Gul MZ, Ahmad F, Kondapi AK, Qureshi IA, Ghazi IA. Antioxidant and antiproliferative activities of Abrus precatorius leaf extracts-an in vitro study. BMC Compl Alt Med 2013;13:1-12.
39. Rathee JS, Hassarajani SA, Chattopadhyay A. Antioxidant activity of Mammea longifolia bud extracts. Food Chem 2006;99:436-43.
40. Becana M, Klucas RV. Transition metals in legume root nodules; Iron-dependent free radical production increases during nodule senescence. PNAS 1992;89:8958-62.
41. Sundararajan R, Koduru R. In vitro antioxidant activity on roots of Limnophila heterophylla. Free Radic Antioxid 2016;6:178-85.
42. Saiaha H, Allemb R, zohra-el-kebira F. Antioxidant and antibacterial activities of six Algerian medicinal plants. Int J Pharm Pharm Sci 2016;8:367-74.
43. Singh P, Singh RL, Kakkar P. Antioxidant, DNA damage protective and hepatoprotective activities of Amorphophallus campanulatus. Int J Pharm Pharm Sci 2016;8:330-8.
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