ANTITUBERCULOSIS ACTIVITY OF EXTRACT AND FRACTIONS OF TINOSPORA CRISPA AGAINST MYCOBACTERIUM TUBERCULOSIS H37RV USING MYCOBACTERIA GROWTH INDICATOR TUBE AND AGAR PROPORTION METHOD

  • Retno Wahyuningrum Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Indonesia.
  • Ritmaleni Ritmaleni Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Indonesia.
  • Tatang Irianti Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Indonesia.
  • Subagus Wahyuono Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Indonesia.
  • Takushi Kaneko Tuberculosis Alliance, New York, United States.

Abstract

 Objective: The increasing incidence of multidrug-resistant tuberculosis (TB) has created a need to discover a new anti-TB drug candidates. The aim of this study was to screen extract and fractions of Tinospora crispa for activity against Mycobacterium tuberculosis H37Rv.

Methods: The dried and pulverized T. crispa stem was extracted by maceration method using ethanol (96%). The anti-TB activity was carried out using mycobacteria growth indicator tube (MGIT) system and agar proportion method with Lowenstein–Jensen (LJ) medium.

Result: The result of this study showed that ethanolic extract and fractions of T. Crispa did not exhibit anti-TB activity in the range of 100–1000 μg/ml with MGIT method, while with agar proportion method, there were M. tuberculosis colonies growth on the LJ containing 1000 μg/ml extract slants.

Conclusion: The tested extract and fractions of T. crispa have no anti-TB activity against M. tuberculosis until 1000 μg/ml.

Keywords: Mycobacterium tuberculosis, Tinospora crispa, Tuberculosis activity.

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How to Cite
Wahyuningrum, R., R. Ritmaleni, T. Irianti, S. Wahyuono, and T. Kaneko. “ANTITUBERCULOSIS ACTIVITY OF EXTRACT AND FRACTIONS OF TINOSPORA CRISPA AGAINST MYCOBACTERIUM TUBERCULOSIS H37RV USING MYCOBACTERIA GROWTH INDICATOR TUBE AND AGAR PROPORTION METHOD”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 3, Mar. 2018, pp. 132-5, doi:10.22159/ajpcr.2018.v11i3.22587.
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